breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR2168732_R1.good.fq1,332,87688,614,511100.0%66.5 bases68 bases95.3%
errorsSRR2168732_R2.good.fq1,332,87588,614,443100.0%66.5 bases68 bases94.4%
total2,665,751177,228,954100.0%66.5 bases68 bases94.9%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65236.33.6100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000002008
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500054
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.002

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.83706

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input16:17:27 03 Mar 202016:18:01 03 Mar 202034 seconds
Read alignment to reference genome16:18:02 03 Mar 202016:22:59 03 Mar 20204 minutes 57 seconds
Preprocessing alignments for candidate junction identification16:22:59 03 Mar 202016:23:37 03 Mar 202038 seconds
Preliminary analysis of coverage distribution16:23:37 03 Mar 202016:24:35 03 Mar 202058 seconds
Identifying junction candidates16:24:35 03 Mar 202016:24:36 03 Mar 20201 second
Re-alignment to junction candidates16:24:36 03 Mar 202016:25:22 03 Mar 202046 seconds
Resolving best read alignments16:25:22 03 Mar 202016:26:16 03 Mar 202054 seconds
Creating BAM files16:26:16 03 Mar 202016:27:18 03 Mar 20201 minute 2 seconds
Tabulating error counts16:27:18 03 Mar 202016:27:33 03 Mar 202015 seconds
Re-calibrating base error rates16:27:33 03 Mar 202016:27:34 03 Mar 20201 second
Examining read alignment evidence16:27:34 03 Mar 202016:30:37 03 Mar 20203 minutes 3 seconds
Polymorphism statistics16:30:37 03 Mar 202016:30:38 03 Mar 20201 second
Output16:30:38 03 Mar 202016:30:54 03 Mar 202016 seconds
Total 13 minutes 26 seconds