breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,811,215 Δ1 bp coding (446/531 nt) mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 3,413,571 T→C intergenic (‑106/‑293) acrS ← / → acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,579,269 Δ4,159 bp IS1‑mediated [yhhW]yrhA [yhhW], yhhX, ryhB, yhhY, yhhZ, yrhA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 285199 285279 81 6 [4] [4] 6 yagF/yagG CP4‑6 prophage; dehydratase family protein/CP4‑6 prophage; putative sugar transporter
* * ÷ NC_000913 583487 583574 88 6 [4] [2] 6 tfaX/appY pseudogene, DLP12 prophage;Phage or Prophage Related/global transcriptional activator; DLP12 prophage
* * ÷ NC_000913 584451 584473 23 10 [5] [2] 7 appY/ompT global transcriptional activator; DLP12 prophage/DLP12 prophage; outer membrane protease VII; outer membrane protein 3b
* * ÷ NC_000913 1212099 1212163 65 6 [5] [3] 7 iraM/ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
* * ÷ NC_000913 1530046 1530118 73 6 [4] [5] 8 ydcD putative immunity protein for RhsE
* * ÷ NC_000913 1754713 1754833 121 6 [5] [4] 6 ydhY/ydhZ putative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex/uncharacterized protein
* * ÷ NC_000913 2057582 2057693 112 6 [5] [5] 6 yeeN/asnW UPF0082 family protein/tRNA‑Asn
* * ÷ NC_000913 2774967 2775054 88 7 [5] [5] 6 [yfjW] [yfjW]
* * ÷ NC_000913 2995169 2995285 117 6 [5] [4] 6 ygeK/ygeN pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator/pseudogene, orgB family, part of T3SS PAI ETT2 remnant
* * ÷ NC_000913 3423720–3424234 3424589–3424238 5–870 7 [5] [2] 7 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3455440 3455478 39 6 [5] [5] 7 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic
* * ÷ NC_000913 3799171 3799329 159 8 [5] [5] 6 [waaU] [waaU]
* * ÷ NC_000913 3804359 3804514 156 8 [2] [5] 6 waaS lipopolysaccharide rhamnose:KdoIII transferase; lipopolysaccharide core biosynthesis protein
* * ÷ NC_000913 3805042 3805128 87 6 [5] [4] 6 [waaS] [waaS]
* * ÷ NC_000913 4304528 4304579 52 8 [0] [5] 6 ytcA/yjcS putative inner membrane efflux pump‑associated DUF1656 family protein/metallo‑beta‑lactamase superfamily protein
* * ÷ NC_000913 4326975 4327062 88 6 [5] [5] 6 yjdM/crfC zinc‑ribbon family protein/clamp‑binding sister replication fork colocalization protein, dynamin‑related
* * ÷ NC_000913 4340599 4340676 78 6 [5] [4] 6 adiA/melR arginine decarboxylase/melibiose operon transcriptional regulator; autoregulator

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 5565 =NA (NA)3 (0.090)
+ATTG
3/122 2.8 NA noncoding (1/105 nt) RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 = 4008432 NA (NA)intergenic (+55/+7) rhtC/rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
* ? NC_000913 = 120780543 (1.210)12 (0.450) 9/98 0.9 33.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 16 (0.600)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 405413714 (0.390)4 (0.130) 4/112 2.3 22.2% coding (1142/1410 nt) glnG fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
?NC_000913 = 4054157 16 (0.520)coding (1122/1410 nt) glnG fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein