Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	486,122	IS1 (–) +8 bp	coding (362‑369/648 nt)	acrR →	transcriptional repressor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 258675	NA (NA)	38 (1.690)	17/132	0.2	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	486122 =	0 (0.000)					coding (362/648 nt)	acrR	transcriptional repressor
*	?	NC_000913	279163 =	NA (NA)	26 (1.180)	16/130	0.2	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 486129	0 (0.000)					coding (369/648 nt)	acrR	transcriptional repressor

GCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/279229‑279163 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGTTCTTCTGTC  <  NC_000913/486129‑486063                                                                                                                                        GCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCC                                                                    >  2:239426/1‑68         ACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCC                                                            >  1:311566/1‑68           AAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGA                                                          >  1:455847/1‑68                     GGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCA                                                >  2:816887/1‑68                     GGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCA                                                >  1:577580/1‑68                       CATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATT                                              >  1:193504/1‑68                          TATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGT                                           <  2:749387/68‑1                          TATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGT                                           <  2:139818/68‑1                            TCTGGACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGG                                         >  1:287882/1‑68                               GAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAAT                                      <  1:327684/68‑1                               GAACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAAT                                      <  2:65674/68‑1                               GAACATAAAACACTATCAATAAGTCGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAAT                                      <  1:333337/68‑1                                AACATAAAACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATA                                     <  2:239484/68‑1                                        ACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCC                             <  1:353687/68‑1                                        ACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCC                             >  2:610781/1‑68                                        ACACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCC                             >  1:282335/1‑68                                         CACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCA                            >  2:209111/1‑68                                         CACTATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCA                            >  1:763756/1‑68                                             ATCAATAAGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAA                        >  1:66681/1‑68                                                    AGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGA                 >  2:414668/1‑68                                                    AGTTGGAGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGA                 >  1:168174/1‑68                                                          AGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGT           <  2:282336/68‑1                                                          AGTCATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGT           <  2:455848/68‑1                                                              ATTACCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGTTCTT       <  1:414667/68‑1                                                                  CCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGTTCTTCTGT   >  2:534664/1‑68                                                                  CCCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGTTCTTCTGT   >  1:467511/1‑68                                                                   CCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGTTCTTCTGTC  >  2:374628/1‑68                                                                   CCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGTTCTTCTGTC  >  2:378638/1‑68                                                                                                                                        GCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/279229‑279163 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCATTTCTCCGACAAATTCGCATTTGTGGAATATAATCTCCATCAATAATCGACGCCGTTCTTCTGTC  <  NC_000913/486129‑486063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.