breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR_1240949_R1.good.fq1,757,703388,943,852100.0%221.3 bases250 bases95.6%
errorsSRR_1240949_R2.good.fq1,757,703388,943,852100.0%221.3 bases250 bases92.5%
total3,515,406777,887,704100.0%221.3 bases250 bases94.0%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_007779_cfb4,646,332165.34.5100.0%Escherichia coli str. K-12 substr. W3110, complete genome.
total4,646,332100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000046749
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500035
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.004

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_007779_cfb0.71652

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input07:08:50 01 Apr 202007:09:51 01 Apr 20201 minute 1 second
Read alignment to reference genome07:09:52 01 Apr 202007:24:07 01 Apr 202014 minutes 15 seconds
Preprocessing alignments for candidate junction identification07:24:07 01 Apr 202007:25:15 01 Apr 20201 minute 8 seconds
Preliminary analysis of coverage distribution07:25:15 01 Apr 202007:28:43 01 Apr 20203 minutes 28 seconds
Identifying junction candidates07:28:43 01 Apr 202007:29:50 01 Apr 20201 minute 7 seconds
Re-alignment to junction candidates07:29:50 01 Apr 202007:33:39 01 Apr 20203 minutes 49 seconds
Resolving best read alignments07:33:39 01 Apr 202007:35:29 01 Apr 20201 minute 50 seconds
Creating BAM files07:35:29 01 Apr 202007:38:23 01 Apr 20202 minutes 54 seconds
Tabulating error counts07:38:23 01 Apr 202007:39:32 01 Apr 20201 minute 9 seconds
Re-calibrating base error rates07:39:32 01 Apr 202007:39:33 01 Apr 20201 second
Examining read alignment evidence07:39:33 01 Apr 202007:51:32 01 Apr 202011 minutes 59 seconds
Polymorphism statistics07:51:32 01 Apr 202007:51:33 01 Apr 20201 second
Output07:51:33 01 Apr 202007:52:04 01 Apr 202031 seconds
Total 43 minutes 13 seconds