breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 62,127 +G coding (1138/2907 nt) rapA ← RNA polymerase‑associated helicase protein
RA 547,694 A→G intergenic (+123/‑1156) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 547,835 +G intergenic (+264/‑1015) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolatedehydrogenase/5,10‑methylene ‑tetrahydrofolatecyclohydrolase
MC JC 654,214 Δ1,199 bp insH_12 insH_12
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,093,686 T→C V130A (GTA→GCA)  ycdT → diguanylate cyclase
RA 1,197,797 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,809 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,822 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,824 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,854 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,857 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,860 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase,‑specific for NADP+
MC JC 1,301,041 Δ1,336 bp insC_8insD_10 insC_8, insD_10
RA 1,519,222 G→T V40V (GTG→GTT ydcX → inner membrane protein
RA 1,669,601 Δ1 bp coding (678/1221 nt) dgsA ← DNA‑binding transcriptional repressor
RA 1,979,957 G→T intergenic (‑37/+398) flhD ← / ← insH_10 DNA‑binding transcriptional dual regulator/IS5 element protein
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
MC JC 2,176,983 Δ1,199 bp insH_11 insH_11
RA 2,245,455 A→G S283P (TCG→CCG)  yeiB ← inner membrane protein
MC JC 2,320,795 Δ1,336 bp insD_9insC_5 insD_9, insC_5
RA 2,382,028 C→A D79Y (GAC→TAC)  menH ← peptidase
RA 2,866,111 A→G intergenic (‑21/+159) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/outer membrane lipoprotein
MC JC 3,261,134 Δ1,199 bp insH_16[tdcD] insH_16, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  yhaC → hypothetical protein
RA 3,449,813 C→A V507L (GTA→TTA)  rpoC ← RNA polymerase, beta prime subunit
RA 3,622,620 G→T R337S (CGT→AGT)  metE ← 5‑methyltetrahydropteroyltriglutamate‑homocystein e S‑methyltransferase
MC JC 3,742,936 Δ1,336 bp insD_8insC insD_8, insC
MC JC 3,746,911 Δ1,199 bp insH_13 insH_13
MC JC 3,750,591 Δ1,199 bp insH_14 insH_14
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) dctA → C4‑dicarboxylic acid, orotate and citratetransporter
RA 4,038,630 C→T A101V (GCG→GTG)  ftsX → transporter subunit
RA 4,150,184 A→G intergenic (+34/‑52) pabA → / → argD aminodeoxychorismate synthase, subunit II/bifunctional acetylornithineaminotransferase/succinyldiaminopimelate aminotransferase
MC JC 4,310,363 Δ1,199 bp insH_15[alsK] insH_15, [alsK]
RA 4,342,252 C→G R119P (CGC→CCC)  adiY ← DNA‑binding transcriptional activator
RA 4,371,274 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,371,467 G→T intergenic (‑202/+104) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779_cfb 1197869 1213099 15231 54 [49] [52] 53 [icd]–mcrA [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,cohE,croE,ymfL,ymfM,ymfN,ymfR,ymfO,ymfP,ymfQ,stfP,tfaP,tfaE,pinE,mcrA
* * ÷ NC_007779_cfb 4362440 4366466 4027 142 [0] [2] 144 [cadA]–[cadC] [cadA],cadB,[cadC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779_cfb 380484 =NA (NA)105 (0.880) 90/438 0.5 NA intergenic (‑418/‑46) yaiO/insC_2 hypothetical protein/IS2 insertion element repressor InsA
?NC_007779_cfb = 381407 NA (NA)coding (510/906 nt) insD IS2 insertion element transposase InsAB'
* ? NC_007779_cfb 381401 =NA (NA)134 (1.140) 109/434 0.1 NA coding (504/906 nt) insD IS2 insertion element transposase InsAB'
?NC_007779_cfb = 381812 NA (NA)coding (303/387 nt) yaiF hypothetical protein
* ? NC_007779_cfb = 3186081NA (NA)116 (0.980) 94/436 0.4 98.3% coding (26/2466 nt) yqiG outer membrane usher protein
?NC_007779_cfb 3512753 = 2 (0.020)coding (529/1026 nt) cytR DNA‑binding transcriptional dual regulator
* ? NC_007779_cfb = 35127592 (0.020)93 (0.790) 79/436 0.9 97.9% coding (535/1026 nt) cytR DNA‑binding transcriptional dual regulator
?NC_007779_cfb = 3744262 NA (NA)intergenic (‑90/+31) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
* ? NC_007779_cfb = 3744263NA (NA)129 (1.090) 107/438 0.2 97.0% intergenic (‑91/+30) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
?NC_007779_cfb 3903274 = 4 (0.030)coding (37/825 nt) bax hypothetical protein
* ? NC_007779_cfb = 39032791 (0.010)99 (0.840) 84/436 0.7 99.0% coding (42/825 nt) bax hypothetical protein
?NC_007779_cfb = 4504190 NA (NA)intergenic (+10/‑89) insD_3/yjgW IS2 insertion element transposase InsAB'/hypothetical protein
* ? NC_007779_cfb 3987185 =NA (NA)141 (1.190) 118/438 0.1 98.6% intergenic (‑731/‑32) slp/insH outer membrane lipoprotein/IS5 transposase and trans‑activator
?NC_007779_cfb 4366467 = 2 (0.020)coding (146/1539 nt) cadC DNA‑binding transcriptional activator
* ? NC_007779_cfb = 4311556NA (NA)106 (0.900) 85/436 0.6 99.1% coding (922/1029 nt) alsK D‑allose kinase
?NC_007779_cfb 4540915 = 1 (0.010)intergenic (+204/+379) yjhR/nanS frameshift suppressor/hypothetical protein
* ? NC_007779_cfb = 4311557NA (NA)140 (1.180) 104/438 0.2 100% coding (921/1029 nt) alsK D‑allose kinase
?NC_007779_cfb = 4362439 0 (0.000)coding (857/2148 nt) cadA lysine decarboxylase 1