breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 547,694 A→G E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,835 +G coding (255/261 nt) Y75_RS02655 → hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
MC JC 654,214 Δ1,199 bp Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS porin OmpF/asparagine‑‑tRNA ligase
RA 1,093,686 T→C V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,197,797 C→T H366H (CAC→CAT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,809 C→T T370T (ACC→ACT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,822 T→C L375M (TTA→CTG)  icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,824 A→G L375M (TTA→CTG icd → NADP‑dependent isocitrate dehydrogenase
MC JC 1,301,041 Δ1,336 bp Y75_RS06530Y75_RS06530 Y75_RS06530, Y75_RS06530
RA 1,519,222 G→T V40V (GTG→GTT Y75_RS07640 → orphan toxin OrtT
RA 1,669,601 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,979,957 G→T intergenic (‑46/+398) flhD ← / ← Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← H48 family flagellin FliC
MC JC 2,176,983 Δ1,199 bp Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,245,455 A→G S283P (TCG→CCG)  Y75_RS11290 ← DUF418 family protein
MC JC 2,320,795 Δ1,336 bp Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,866,111 A→G pseudogene (97/993 nt) rpoS ← RNA polymerase sigma factor RpoS
MC JC 3,261,134 Δ1,199 bp Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  Y75_RS16240 → YhaC family protein
MC JC 3,742,936 Δ1,336 bp Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,750,591 Δ1,199 bp Y75_RS18600 Y75_RS18600
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) Y75_RS19595 → C4‑dicarboxylate transporter DctC
MC JC 4,310,363 Δ1,199 bp Y75_RS21310[alsK] Y75_RS21310, [alsK]
RA 4,371,274 (A)5→3 pseudogene (179‑180/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1197843 1213102 15260 87 [85] [85] 86 [icd]–[Y75_RS23470] [icd],Y75_RS05945,Y75_RS05950,lit,Y75_RS05960,Y75_RS05965,Y75_RS24820,Y75_RS23460,Y75_RS05970,Y75_RS05975,Y75_RS05980,ymfL,Y75_RS23465,Y75_RS05995,Y75_RS06000,Y75_RS06005,Y75_RS06010,Y75_RS06015,Y75_RS06020,Y75_RS06025,Y75_RS06030,Y75_RS06035,pinE,Y75_RS06045,[Y75_RS23470]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 380484 =NA (NA)170 (1.150) 142/496 0.0 NA coding (28/444 nt) Y75_RS01845 transferase
?NC_007779 = 381407 NA (NA)coding (518/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 = 3186081NA (NA)156 (1.060) 120/494 0.3 98.7% coding (26/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 3512753 = 2 (0.010)coding (529/1026 nt) cytR DNA‑binding transcriptional regulator CytR
* ? NC_007779 = 35127597 (0.050)144 (0.980) 118/494 0.4 95.4% coding (535/1026 nt) cytR DNA‑binding transcriptional regulator CytR
?NC_007779 = 3744262 NA (NA)intergenic (‑45/+31) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
* ? NC_007779 = 3744263NA (NA)183 (1.240) 148/496 0.0 100% intergenic (‑46/+30) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
?NC_007779 3903274 = 0 (0.000)coding (37/825 nt) Y75_RS19350 protein bax
* ? NC_007779 = 39032791 (0.010)130 (0.880) 116/494 0.4 99.2% coding (42/825 nt) Y75_RS19350 protein bax
?NC_007779 = 4504190 NA (NA)intergenic (+10/+167) Y75_RS22280/Y75_RS22285 IS3‑like element IS2 family transposase/phosphoethanolamine transferase
* ? NC_007779 = 4311556NA (NA)157 (1.070) 118/494 0.4 96.9% intergenic (+145/+1) Y75_RS21310/alsK IS5‑like element IS5 family transposase/allose kinase
?NC_007779 4540915 = 5 (0.030)intergenic (+204/+379) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase