breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 33 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077791,097,5800CT33.3% 68.7 / 53.0 60intergenic (+329/+45)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077794,609,0240CT30.7% 182.5 / 38.6 101S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NC_0077793,205,0910CT28.4% 155.2 / 37.5 88intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_007779753,5470TA27.9% 184.9 / 25.1 79intergenic (‑330/+60)Y75_RS03720/gltAtype 1 fimbrial protein/citrate (Si)‑synthase
*NC_0077793,205,1020CA26.7% 150.5 / 22.6 91intergenic (‑190/‑17)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_0077794,291,3090TA26.6% 252.4 / 43.5 109intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_0077794,585,3140CT26.3% 323.9 / 43.9 137E277K (GAA→AAA) hsdStype I restriction‑modification system specificity subunit
*NC_0077792,004,0550AC26.0% 240.5 / 10.4 101intergenic (‑129/+192)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NC_0077791,437,4410GA25.4% 256.6 / 54.8 114intergenic (‑108/+33)uspF/ompNuniversal stress protein UspF/porin OmpN
*NC_0077794,585,3220GT25.0% 340.1 / 33.0 125S274* (TCA→TAA) hsdStype I restriction‑modification system specificity subunit
*NC_0077792,488,7140TA24.4% 282.5 / 32.6 123L24F (TTA→TTTemrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_0077792,627,2810AC24.2% 222.0 / 10.6 96V105G (GTC→GGC) pdeFcyclic‑guanylate‑specific phosphodiesterase PdeF
*NC_0077791,097,5400CA23.8% 149.6 / 29.5 64intergenic (+289/+85)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077793,974,7130GT23.4% 356.4 / 33.9 142A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NC_0077793,157,5490TC22.9% 272.9 / 22.5 105intergenic (+9/+34)yqhH/Y75_RS15695lipoprotein YqhH/YgiQ family radical SAM protein
*NC_0077794,585,3200CT22.8% 339.5 / 16.1 127E275K (GAA→AAA) hsdStype I restriction‑modification system specificity subunit
*NC_0077791,826,7750GA22.7% 221.3 / 18.0 88intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone
*NC_0077793,004,6340CA22.0% 335.9 / 24.6 118intergenic (‑192/‑287)Y75_RS14955/ygeWsigma‑54‑dependent transcriptional regulator/knotted carbamoyltransferase YgeW
*NC_0077793,004,7060GA22.0% 285.4 / 39.2 118intergenic (‑264/‑215)Y75_RS14955/ygeWsigma‑54‑dependent transcriptional regulator/knotted carbamoyltransferase YgeW
*NC_0077791,246,8110GT21.9% 320.9 / 19.9 114L25F (TTG→TTTY75_RS06255hypothetical protein

Marginal new junction evidence (lowest skew 10 of 11 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 4310363 =NA (NA)105 (0.710) 71/496 3.4 99.1% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 = 4540919 1 (0.010)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 381401 =NA (NA)77 (0.530) 63/492 4.4 NA coding (512/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381812 NA (NA)pseudogene (174/174 nt) Y75_RS23175 acetyltransferase
* ? NC_007779 = 4547370134 (0.910)18 (0.130) 18/478 15.4 11.7% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 = 4547666 144 (1.010)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 4547362 =135 (0.920)18 (0.130) 18/478 15.4 11.6% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 4547676 = 144 (1.010)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 = 294612343 (0.290)8 (0.080) 8/330 15.8 16.4% intergenic (+4/‑30) Y75_RS14670/Y75_RS14675 tRNA‑Met/tRNA‑Met
?NC_007779 2946344 = 53 (0.540)intergenic (+5/+69) Y75_RS14680/Y75_RS14685 tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase AmiC
* ? NC_007779 = 69716397 (0.660)7 (0.070) 7/340 16.9 8.7% intergenic (‑1/+15) Y75_RS03450/Y75_RS03455 tRNA‑Met/tRNA‑Gln
?NC_007779 697557 = 81 (0.800)intergenic (‑2/+378) Y75_RS03470/asnB tRNA‑Met/asparagine synthase B
* ? NC_007779 = 2069490173 (1.170)76 (0.520) 15/496 17.4 46.6% intergenic (‑32/+1) Y75_RS10470/Y75_RS24935 IS5‑like element IS5 family transposase/hypothetical protein
?NC_007779 = 4540919 1 (0.010)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 1413613 =157 (1.060)5 (0.040) 5/444 22.2 3.2% coding (50/936 nt) ttcA tRNA 2‑thiocytidine(32) synthetase TtcA
?NC_007779 = 1436672 160 (1.210)intergenic (+39/+227) Y75_RS24865/uspF hypothetical protein/universal stress protein UspF
* ? NC_007779 3406257 =161 (1.090)3 (0.020) 3/472 24.9 1.9% coding (486/1350 nt) accC acetyl‑CoA carboxylase biotin carboxylase subunit
?NC_007779 = 3406253 153 (1.090)coding (482/1350 nt) accC acetyl‑CoA carboxylase biotin carboxylase subunit
* ? NC_007779 = 2072408NA (NA)6 (0.040) 3/492 25.5 NA intergenic (‑46/‑3) Y75_RS10480/Y75_RS10490 IS3‑like element IS2 family transposase/TonB‑dependent receptor plug domain‑containing protein
?NC_007779 = 2105081 NA (NA)intergenic (‑33/+2) Y75_RS10660/Y75_RS10665 IS5‑like element IS5 family transposase/glycosyltransferase