breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 50 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077793,004,6680CT37.5% 111.7 / 35.8 72intergenic (‑226/‑253)Y75_RS14955/ygeWsigma‑54‑dependent transcriptional regulator/knotted carbamoyltransferase YgeW
*NC_0077791,097,5800CT36.1% 73.7 / 73.6 72intergenic (+329/+45)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077794,291,3090TA32.4% 127.5 / 37.4 68intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_007779627,8750TG31.2% 131.4 / 10.8 64G34G (GGT→GGGdhbA2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
*NC_0077794,609,0240CT31.2% 145.4 / 28.1 80S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NC_0077792,488,7000GA30.7% 207.9 / 30.0 101S29L (TCA→TTA) emrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_007779753,5470TA29.8% 177.4 / 31.2 84intergenic (‑330/+60)Y75_RS03720/gltAtype 1 fimbrial protein/citrate (Si)‑synthase
*NC_0077792,331,1200TG29.5% 114.9 / 20.9 62G114G (GGT→GGGY75_RS11690acetyl‑CoA acetyltransferase
*NC_0077791,826,7750GA28.8% 157.6 / 27.4 73intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone
*NC_0077793,176,4040CA28.2% 179.9 / 32.1 85E55* (GAG→TAG) nudFADP‑ribose diphosphatase
*NC_0077791,097,5400CA26.9% 116.0 / 22.3 52intergenic (+289/+85)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077793,205,0910CT26.7% 166.3 / 40.7 90intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_0077793,974,7130GT26.7% 201.8 / 16.7 76A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NC_0077791,294,0350TA25.8% 236.3 / 30.9 97intergenic (+93/+51)galU/hnsUTP‑‑glucose‑1‑phosphate uridylyltransferase GalU/DNA‑binding transcriptional regulator H‑NS
*NC_007779127,2090TG25.6% 192.6 / 14.9 86G505G (GGT→GGGaceFpyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
*NC_0077793,189,7590CT25.5% 222.7 / 23.2 95P158S (CCA→TCA) Y75_RS15860fimbrial protein
*NC_0077791,581,4710CA25.2% 265.6 / 29.3 103C345F (TGC→TTC) Y75_RS07900anaerobic sulfatase maturase
*NC_00777929,4960TA24.7% 195.4 / 24.8 77intergenic (+301/‑155)dapB/Y75_RS001654‑hydroxy‑tetrahydrodipicolinate reductase/glutamine‑hydrolyzing carbamoyl‑phosphate synthase small subunit
*NC_0077792,488,7070CA24.5% 262.3 / 14.1 102A27S (GCG→TCG) emrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_0077792,902,3480CA24.3% 184.8 / 31.8 70intergenic (+318/+1055)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 4310363 =NA (NA)60 (0.560) 50/498 3.4 96.8% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 = 4540919 2 (0.020)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 381401 =NA (NA)48 (0.450) 42/494 4.6 NA coding (512/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381812 NA (NA)pseudogene (174/174 nt) Y75_RS23175 acetyltransferase
* ? NC_007779 = 4547370113 (1.050)21 (0.200) 21/480 9.6 17.7% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 = 4547666 86 (0.830)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 4547362 =106 (0.980)16 (0.150) 16/480 11.5 14.5% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 4547676 = 87 (0.840)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 = 2069490107 (0.990)57 (0.530) 11/498 14.3 51.1% intergenic (‑32/+1) Y75_RS10470/Y75_RS24935 IS5‑like element IS5 family transposase/hypothetical protein
?NC_007779 = 4540919 2 (0.020)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase