breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 49 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077791,097,5800CT32.8% 63.6 / 63.2 67intergenic (+329/+45)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077794,291,3090TA29.3% 189.8 / 43.1 92intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_0077793,974,7130GT28.9% 314.5 / 44.9 135A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NC_0077794,609,0240CT27.2% 160.6 / 28.6 81S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NC_0077793,004,6680CT26.2% 284.5 / 34.9 126intergenic (‑226/‑253)Y75_RS14955/ygeWsigma‑54‑dependent transcriptional regulator/knotted carbamoyltransferase YgeW
*NC_0077792,902,3480CA26.0% 348.1 / 53.6 123intergenic (+318/+1055)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_007779753,5470TA25.9% 268.9 / 31.8 108intergenic (‑330/+60)Y75_RS03720/gltAtype 1 fimbrial protein/citrate (Si)‑synthase
*NC_0077792,488,7140TA25.3% 365.9 / 43.4 158L24F (TTA→TTTemrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_00777929,4960TA25.2% 299.8 / 45.1 119intergenic (+301/‑155)dapB/Y75_RS001654‑hydroxy‑tetrahydrodipicolinate reductase/glutamine‑hydrolyzing carbamoyl‑phosphate synthase small subunit
*NC_0077794,261,2880TG25.0% 180.3 / 11.4 81V195G (GTT→GGT) lexArepressor LexA
*NC_007779658,0980AC24.7% 220.0 / 19.2 97V88G (GTG→GGG) crcBfluoride efflux transporter CrcB
*NC_0077791,437,4410GA23.7% 286.3 / 38.3 114intergenic (‑108/+33)uspF/ompNuniversal stress protein UspF/porin OmpN
*NC_0077792,188,5330TG23.5% 240.7 / 23.3 102T35P (ACC→CCC) thiMhydroxyethylthiazole kinase
*NC_0077793,475,5330CT23.5% 261.8 / 33.3 115E10K (GAA→AAA) fabRHTH‑type transcriptional repressor FabR
*NC_0077792,983,3790AG23.3% 171.4 / 13.2 73I290I (ATT→ATCY75_RS14830acetyl‑CoA C‑acetyltransferase
*NC_0077792,488,7000GA23.3% 388.0 / 35.6 159S29L (TCA→TTA) emrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_007779558,4040GA23.2% 329.7 / 20.6 142E70K (GAA→AAA) fimCtype 1 fimbria chaperone FimC
*NC_0077791,015,9380GT22.8% 301.1 / 22.8 114intergenic (+57/‑199)pqiC/rmfmembrane integrity‑associated transporter subunit PqiC/ribosome modulation factor
*NC_00777929,4860CA22.7% 322.6 / 37.0 110intergenic (+291/‑165)dapB/Y75_RS001654‑hydroxy‑tetrahydrodipicolinate reductase/glutamine‑hydrolyzing carbamoyl‑phosphate synthase small subunit
*NC_0077792,004,0550AC22.6% 212.2 / 14.7 84intergenic (‑129/+192)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 4310363 =NA (NA)63 (0.460) 56/496 4.7 100% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 = 4540919 0 (0.000)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 1413613 =131 (0.960)15 (0.120) 15/444 14.6 10.7% coding (50/936 nt) ttcA tRNA 2‑thiocytidine(32) synthetase TtcA
?NC_007779 = 1436672 134 (1.100)intergenic (+39/+227) Y75_RS24865/uspF hypothetical protein/universal stress protein UspF
* ? NC_007779 = 2069490150 (1.100)59 (0.430) 14/496 16.6 44.0% intergenic (‑32/+1) Y75_RS10470/Y75_RS24935 IS5‑like element IS5 family transposase/hypothetical protein
?NC_007779 = 4540919 0 (0.000)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase