breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 44 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077791,097,5800CT32.6% 42.6 / 32.8 43intergenic (+329/+45)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077792,488,7000GA31.7% 233.2 / 40.0 124S29L (TCA→TTA) emrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_0077792,004,0550AC31.6% 193.7 / 25.3 98intergenic (‑129/+192)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NC_0077793,205,0910CT29.6% 173.7 / 42.0 98intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_0077792,004,0480GA27.6% 196.4 / 44.4 98intergenic (‑122/+199)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NC_0077793,475,5330CT27.3% 270.0 / 45.8 132E10K (GAA→AAA) fabRHTH‑type transcriptional repressor FabR
*NC_0077791,826,7750GA27.2% 212.6 / 24.7 92intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone
*NC_0077794,609,0240CT26.6% 217.8 / 36.4 109S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NC_0077793,895,3730TG26.6% 112.9 / 14.8 65D80A (GAC→GCC) yiaN2,3‑diketo‑L‑gulonate transporter large permease YiaN
*NC_0077791,826,7700GA25.6% 196.6 / 26.2 82intergenic (‑119/+84)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone
*NC_0077791,097,5400CA25.5% 117.3 / 21.4 48intergenic (+289/+85)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077793,205,1510GA25.5% 213.2 / 18.9 106V11V (GTG→GTAttdAL(+)‑tartrate dehydratase subunit alpha
*NC_007779753,5470TA24.8% 254.7 / 31.1 101intergenic (‑330/+60)Y75_RS03720/gltAtype 1 fimbrial protein/citrate (Si)‑synthase
*NC_0077793,401,3140TC24.6% 277.6 / 29.7 115D625G (GAT→GGT) csrDRNase E specificity factor CsrD
*NC_0077792,827,1620TG24.3% 182.5 / 27.0 108V257G (GTG→GGG) srlDsorbitol‑6‑phosphate dehydrogenase
*NC_0077792,488,7140TA24.0% 264.4 / 30.5 121L24F (TTA→TTTemrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_0077792,902,3420GT24.0% 268.3 / 14.7 97intergenic (+312/+1061)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077792,902,3480CA24.0% 257.4 / 35.9 96intergenic (+318/+1055)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077794,108,9760TG23.6% 321.6 / 18.9 127Y334D (TAT→GAT) Y75_RS20260DUF4153 domain‑containing protein
*NC_007779634,2890CA23.3% 235.6 / 14.6 87G104V (GGT→GTT) Y75_RS03120ParB‑like nuclease domain‑containing protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 381401 =NA (NA)80 (0.530) 68/492 4.3 NA coding (512/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381812 NA (NA)pseudogene (174/174 nt) Y75_RS23175 acetyltransferase
* ? NC_007779 4310363 =NA (NA)78 (0.510) 68/496 4.4 96.3% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 = 4540919 3 (0.020)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 = 2069490167 (1.090)72 (0.470) 16/496 18.3 45.9% intergenic (‑32/+1) Y75_RS10470/Y75_RS24935 IS5‑like element IS5 family transposase/hypothetical protein
?NC_007779 = 4540919 3 (0.020)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 1413613 =174 (1.130)10 (0.070) 9/444 20.7 6.8% coding (50/936 nt) ttcA tRNA 2‑thiocytidine(32) synthetase TtcA
?NC_007779 = 1436672 120 (0.870)intergenic (+39/+227) Y75_RS24865/uspF hypothetical protein/universal stress protein UspF
* ? NC_007779 4547362 =159 (1.040)4 (0.030) 4/478 25.8 2.6% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 4547676 = 146 (0.990)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 = 461075695 (0.620)4 (0.030) 4/496 26.4 4.2% intergenic (+36/+3) Y75_RS22830/Y75_RS22835 DUF1435 domain‑containing protein/tRNA‑Leu
?NC_007779 4610993 = 87 (0.570)intergenic (‑27/+2) Y75_RS22840/Y75_RS22845 tRNA‑Leu/tRNA‑Leu
* ? NC_007779 1724330 =160 (1.040)3 (0.020) 3/474 26.7 1.9% coding (496/2013 nt) Y75_RS08665 FUSC family protein
?NC_007779 = 1724326 152 (1.040)coding (492/2013 nt) Y75_RS08665 FUSC family protein
* ? NC_007779 = 4547370164 (1.070)3 (0.020) 3/478 26.8 1.9% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 = 4547666 145 (0.980)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA