breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 62,682 +G coding (583/2907 nt) rapA ← RNA polymerase‑associated protein RapA
RA 277,827 C→G G67A (GGC→GCC)  Y75_RS01350 ← ferric ABC transporter ATP‑binding protein
MC JC 490,544 Δ6 bp intergenic (+61/‑87) Y75_RS02410 → / → apt DUF454 family protein/adenine phosphoribosyltransferase
RA 547,694 A→G E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,835 +G coding (255/261 nt) Y75_RS02655 → hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
RA 573,671 T→A intergenic (+109/+289) quuD → / ← Y75_RS02845 antitermination protein QuuD/IS5‑like element IS5 family transposase
MC JC 654,214 Δ1,199 bp Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS porin OmpF/asparagine‑‑tRNA ligase
RA 1,093,686 T→C V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,197,797 C→T H366H (CAC→CAT icd → NADP‑dependent isocitrate dehydrogenase
MC JC 1,301,041 Δ1,336 bp Y75_RS06530Y75_RS06530 Y75_RS06530, Y75_RS06530
RA 1,519,222 G→T V40V (GTG→GTT Y75_RS07640 → orphan toxin OrtT
MC JC 1,618,943 Δ6,115 bp [ydeA][dgcZ] [ydeA], marC, marR, marA, marB, eamA, ydeE, mgtS, [dgcZ]
RA 1,669,601 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,979,957 G→T intergenic (‑46/+398) flhD ← / ← Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← H48 family flagellin FliC
RA 2,093,073 G→A E249K (GAA→AAA)  hisG → ATP phosphoribosyltransferase
MC JC 2,176,983 Δ1,199 bp Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,245,455 A→G S283P (TCG→CCG)  Y75_RS11290 ← DUF418 family protein
MC JC 2,320,795 Δ1,336 bp Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,866,111 A→G pseudogene (97/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 3,241,721 G→T L84M (CTG→ATG)  Y75_RS16095 ← altronate dehydratase
MC JC 3,261,134 Δ1,199 bp Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  Y75_RS16240 → YhaC family protein
MC JC 3,742,936 Δ1,336 bp Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) Y75_RS19595 → C4‑dicarboxylate transporter DctC
RA 4,297,865 +T intergenic (+136/‑206) nrfG → / → gltP heme lyase NrfEFG subunit NrfG/glutamate/aspartate:proton symporter GltP
MC JC 4,310,363 Δ1,199 bp Y75_RS21310[alsK] Y75_RS21310, [alsK]
RA 4,371,274 (A)5→3 pseudogene (179‑180/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
RA 4,547,650 Δ1 bp intergenic (+337/‑145) fimE → / → fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1197804 1213113 15310 92 [90] [91] 92 [icd]–[Y75_RS23470] [icd],Y75_RS05945,Y75_RS05950,lit,Y75_RS05960,Y75_RS05965,Y75_RS24820,Y75_RS23460,Y75_RS05970,Y75_RS05975,Y75_RS05980,ymfL,Y75_RS23465,Y75_RS05995,Y75_RS06000,Y75_RS06005,Y75_RS06010,Y75_RS06015,Y75_RS06020,Y75_RS06025,Y75_RS06030,Y75_RS06035,pinE,Y75_RS06045,[Y75_RS23470]
* * ÷ NC_007779 3987267–3988384 3988834 451–1568 92 [91] [1] 130 [Y75_RS19725]–[Y75_RS19730] [Y75_RS19725],[Y75_RS19730]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 89166 =3 (0.020)151 (0.980) 116/496 0.6 98.0% intergenic (+134/‑468) cra/mraZ catabolite repressor/activator/division/cell wall cluster transcriptional repressor MraZ
?NC_007779 = 274373 NA (NA)intergenic (‑32/‑176) Y75_RS01335/mmuP IS5‑like element IS5 family transposase/S‑methylmethionine permease
* ? NC_007779 = 891691 (0.010)153 (1.000) 120/496 0.4 99.4% intergenic (+137/‑465) cra/mraZ catabolite repressor/activator/division/cell wall cluster transcriptional repressor MraZ
?NC_007779 273179 = NA (NA)intergenic (+2/+146) Y75_RS01330/Y75_RS01335 hypothetical protein/IS5‑like element IS5 family transposase
* ? NC_007779 380484 =NA (NA)126 (0.820) 105/496 1.0 NA coding (28/444 nt) Y75_RS01845 transferase
?NC_007779 = 381407 NA (NA)coding (518/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 1397758 =NA (NA)147 (0.960) 125/496 0.3 99.3% intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
?NC_007779 1549542 = 1 (0.010)coding (428/3048 nt) fdnG formate dehydrogenase‑N subunit alpha
* ? NC_007779 = 1398952NA (NA)170 (1.110) 125/496 0.3 97.7% intergenic (+146/‑127) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
?NC_007779 = 1549545 4 (0.030)coding (431/3048 nt) fdnG formate dehydrogenase‑N subunit alpha
* ? NC_007779 1822768 =5 (0.030)179 (1.170) 128/494 0.2 97.3% coding (161/1359 nt) chbC PTS N,N'‑diacetylchitobiose transporter subunit IIC
?NC_007779 2176984 = NA (NA)intergenic (‑4/‑31) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 = 18227724 (0.030)164 (1.070) 121/496 0.4 97.6% coding (157/1359 nt) chbC PTS N,N'‑diacetylchitobiose transporter subunit IIC
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 = 3186081NA (NA)175 (1.140) 131/494 0.2 97.2% coding (26/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 3512753 = 5 (0.030)coding (529/1026 nt) cytR DNA‑binding transcriptional regulator CytR
* ? NC_007779 3277113 =10 (0.070)166 (1.100)
+TTGAA
140/486 0.1 94.4% coding (257/336 nt) prlF type II toxin‑antitoxin system antitoxin PrlF
?NC_007779 = 3277128 NA (NA)coding (272/336 nt) prlF type II toxin‑antitoxin system antitoxin PrlF
* ? NC_007779 = 35127591 (0.010)143 (0.940) 115/494 0.6 99.3% coding (535/1026 nt) cytR DNA‑binding transcriptional regulator CytR
?NC_007779 = 3744262 NA (NA)intergenic (‑45/+31) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
* ? NC_007779 = 3744263NA (NA)158 (1.030) 134/496 0.2 98.1% intergenic (‑46/+30) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
?NC_007779 3903274 = 3 (0.020)coding (37/825 nt) Y75_RS19350 protein bax
* ? NC_007779 3746912 =NA (NA)158 (1.030) 124/494 0.3 97.5% intergenic (‑4/‑31) Y75_RS18580/Y75_RS18585 tryptophan permease/IS5‑like element IS5 family transposase
?NC_007779 = 3975234 4 (0.030)coding (630/825 nt) gadX acid resistance transcriptional activator GadX
* ? NC_007779 = 37505900 (0.000)127 (0.840) 110/488 0.7 100% intergenic (‑104/‑33) tnaA/Y75_RS18600 tryptophanase/IS5‑like element IS5 family transposase
?NC_007779 3751790 = 0 (0.000)intergenic (+151/+116) Y75_RS18600/tnaC IS5‑like element IS5 family transposase/tryptophanase leader peptide
* ? NC_007779 = 39032793 (0.020)150 (0.980) 125/494 0.3 98.0% coding (42/825 nt) Y75_RS19350 protein bax
?NC_007779 = 4504190 NA (NA)intergenic (+10/+167) Y75_RS22280/Y75_RS22285 IS3‑like element IS2 family transposase/phosphoethanolamine transferase
* ? NC_007779 3975230 =2 (0.010)179 (1.170) 132/496 0.2 98.9% coding (626/825 nt) gadX acid resistance transcriptional activator GadX
?NC_007779 = 3988379 NA (NA)coding (746/783 nt) Y75_RS19730 hypothetical protein
* ? NC_007779 = 3988379NA (NA)129 (0.840) 108/496 0.9 99.2% coding (746/783 nt) Y75_RS19730 hypothetical protein
?NC_007779 3988835 = 1 (0.010)coding (290/783 nt) Y75_RS19730 hypothetical protein
* ? NC_007779 = 4311556NA (NA)127 (0.830) 109/494 0.8 97.0% intergenic (+145/+1) Y75_RS21310/alsK IS5‑like element IS5 family transposase/allose kinase
?NC_007779 4540915 = 4 (0.030)intergenic (+204/+379) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase