breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 56,908 C→A V68L (GTG→TTG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component
RA 982,565 T→A intergenic (‑127/+476) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
JC JC 2,400,257 IS5 (+) +4 bp intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
RA 2,420,353 A→G intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
RA 2,420,394 G→T intergenic (‑127/+94) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
RA 2,528,938 T→A L505H (CTT→CAT)  ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)
JC JC 3,178,254 IS5 (+) +4 bp coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein
MC JC 3,360,110 Δ10,684 bp IS5‑mediated [yhcE]sspA [yhcE], yhcF, yhcG, yhcH, nanK, nanE, nanT, nanA, nanR, dcuD, sspB, sspA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 2084559 2085880 1322 14 [13] [13] 15 [hisG]–[hisC] [hisG], [hisD], [hisC]
* * ÷ CP009273 3612754–3614441 3615544–3614445 5–2791 14 [13] [13] 15 rhsB Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)85 (2.080) 57/290 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 1391495NA (NA)32 (0.780) 24/290 0.9 94.1% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 2083419 2 (0.050)intergenic (‑225/‑58) yefM/hisL antitoxin of the YoeB‑YefM toxin‑antitoxin system/his operon leader peptide
* ? CP009273 2083416 =3 (0.070)52 (1.270) 47/290 0.0 94.5% intergenic (‑222/‑61) yefM/hisL antitoxin of the YoeB‑YefM toxin‑antitoxin system/his operon leader peptide
?CP009273 = 2096424 NA (NA)noncoding (1/1195 nt) IS5 repeat region