Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 41.5% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)10 (0.520) 10/276 NT 44.7% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 12 (0.640)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)7 (0.360) 7/276 NT 36.2% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 12 (0.640)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

CAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  CP009273/2282250‑2282536
                                                                                                                                                    |                                                                                                                                          
cAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGAATTATTGGCGGTCCCATAAACCAGCTGGATATCGTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATCTaag                                                                                                                                            >  1:280853/1‑149 (MQ=255)
  aaTTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCACATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaagga                                                                                                                                          >  1:112972/1‑149 (MQ=255)
  aaTTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCACATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaagga                                                                                                                                          >  1:268058/1‑149 (MQ=255)
       ttttATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTg                                                                                                                                     >  1:182855/1‑149 (MQ=255)
                 tGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGAACAACACCATCAACTGTCTCCCCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCTAGTAAGCg                                                                                                                           >  1:66368/1‑149 (MQ=255)
                        cGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                                    >  2:241819/1‑149 (MQ=255)
                                          tattatTGGCGGTCCCATAAACCCGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTAAACAGTTGATGCCAATGCTATGGGTGAATGTAGGGAAGGGGCGGATAAGCGGGGAAATTCTTCTCGGCTGACTCAg                                                                                                  >  1:256508/1‑149 (MQ=38)
                                                                cAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTg                                                                            <  1:266422/149‑1 (MQ=255)
                                                                  gCTGGATATCTTTTTCCAGGACAACACCATCAAGTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAg                                                                          >  1:208245/1‑149 (MQ=255)
                                                                          tCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGGTTGAGCCGAtttt                                                                  >  2:167109/1‑149 (MQ=255)
                                                                           cTTTTTCCAGGACAACACCATCAACCGTATCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGATATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                                                                 >  1:181099/1‑149 (MQ=255)
                                                                            tttttCCAGGACAACACCATCAACTGTCTCACCTTACTCAACAGTTGATGCCAATGCTATGGGGGAAAATAAGGAAGGTGCGAATCAGCGGGGAAATTGTTCTCGGCTGGCTCAGTCATTGCATTTCTTCCTGTTTGAGCCGAtttttt                                                                >  1:119805/1‑149 (MQ=255)
                                                                                     gACAACACCATCAACTGTCTCACCGTCCTCAACAGTTGATGACAATGATATGAGTGAATATAAGGAAAGTGCGAATAAGCGGGGAAATTCTTCTCGACTGACTGAGTCATTGCATTACTTCATGTTTGAGCCGATTTTTTATCCCTTaa                                                       >  1:21843/1‑149 (MQ=18)
                                                                                                    tGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                        >  2:193831/1‑149 (MQ=255)
                                                                                                      tctcACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGATGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATTCCTTGAATCAGCCt                                      >  1:266643/1‑149 (MQ=255)
                                                                                                                               cAAGGCGATGGTTAATTATAAGGAAGTTTCGAATAAGCCGGTAACTTCTTCTCGGCTGACGCAGTCATTTCCTTTCTTCATCTTTTCTCCGTTTTTGTCTCCCGTCAATGCCTTGAATCAGCCTACTTAGTCCGTTTCTTCGCCATTTa             <  2:66368/149‑1 (MQ=2)
                                                                                                                                aaTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTTAGCCGATTTTTTCTCCCGTAAATGTCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTaa            <  1:307294/149‑1 (MQ=255)
                                                                                                                                aaTGCTATGGGTGAATATAAGGAAGGTGCCAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCGTCGCCATTTaa            >  2:181967/1‑149 (MQ=255)
                                                                                                                                  tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGTGGAAATTCTTCTCGGCTGACTCAGTCATTTCTTTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAgg          <  2:12009/149‑1 (MQ=255)
                                                                                                                                          gTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc  >  1:173824/1‑149 (MQ=35)
                                                                                                                                                    |                                                                                                                                          
CAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  CP009273/2282250‑2282536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.