Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 41.5% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)10 (0.520) 10/276 NT 44.7% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 12 (0.640)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)7 (0.360) 7/276 NT 36.2% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 12 (0.640)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

TATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAG  >  CP009273/2400125‑2400395
                                                                                                                                       |                                                                                                                                       
tatCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTcac                                                                                                                            >  2:38257/1‑149 (MQ=255)
    taaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTg                                                                                                                        >  1:145062‑M2/129‑149 (MQ=255)
     aaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacataaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCAcattat                                                                                                                       >  1:230528‑M2/128‑147 (MQ=255)
               gaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATaa                                                                                                             <  1:44150‑M2/32‑1 (MQ=255)
                aaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAAt                                                                                                            >  2:201655‑M2/117‑149 (MQ=255)
                       ctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGaa                                                                                                     <  2:145062‑M2/40‑1 (MQ=255)
                               ttAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTATTCCGTTATAAGGCCTAAAATAGATcc                                                                                             >  2:108695/1‑148 (MQ=255)
                                       aaggcatttatgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattctctccttccCTAAAAAAGCGACTTACATTGTTCCGGTATAATGCCTGAAGTAGATCACAGGatat                                                                                     <  1:228534‑M2/56‑1 (MQ=255)
                                            cacacaAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCtt                                                                                <  2:154526/149‑1 (MQ=255)
                                                  gggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGa                                                                          >  2:258157‑M2/83‑149 (MQ=255)
                                                      gaaaaaatcggctcaaacatgaagaaatgaaaagactgagtcagccgagaagaatttccccgcttattcgcaccttcaCTAAAAAAGCGACTCACAGTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTTAGGGATCGc                                                                      >  2:138267‑M2/79‑149 (MQ=255)
                                                           ttCCGTTTGTAATGTGCCTCCCGGGCAGTTCCATTCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTACggaaggatcgaacaagtctcttatatgtgatcatgtttttcatctggagccatagaacagggttcatcatga                                                                 >  2:20285‑M2/1‑77 (MQ=255)
                                                             ccATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATAACAGACTAAAACTTCAGGGATCGCATATCTa                                                               >  2:321311/1‑149 (MQ=255)
                                                              cATTTGTAATGTCCCTCCCTGGCAGTTTCATCGGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTAt                                                              <  1:147261/149‑1 (MQ=255)
                                                                       tgtgCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaactta                                                     >  1:143517‑M2/1‑65 (MQ=255)
                                                                        gtgtCTCCCTGGCAGCGTCATCCGGAACCCGGACGAAAATAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgacgcatcaacttac                                                    >  2:289154‑M2/1‑64 (MQ=255)
                                                                            cTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTAGTAAGTTactcactc                                                <  1:38257/149‑1 (MQ=255)
                                                                                atgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATAACAGAATATGTATTCAGGGATTCCAACTCTTTTGAGTTACTCACTGtttt                                            >  1:311636‑M2/53‑149 (MQ=255)
                                                                                  ggCAGTTTCATCCGGAACCAGGAAGAACGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatcttgagccatagaacagggttcatcatgagtcatcaacttaccttcgccgac                                          >  1:184758‑M2/1‑54 (MQ=255)
                                                                                       tttCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagagcatgtttgtcatctggagccatagaaaagggttcatcatgagtcatcaacttaccttcgccgacagtga                                     <  2:10342‑M2/149‑101 (MQ=255)
                                                                                         tCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATg                                   >  2:206792/1‑149 (MQ=255)
                                                                                           aTCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTAAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGAc                                 >  1:39103/1‑149 (MQ=255)
                                                                                            tCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGAct                                >  2:284735/1‑148 (MQ=255)
                                                                                            tCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTACGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACa                                >  1:289478/1‑149 (MQ=255)
                                                                                                     cGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcagcaacttatcttcgccgacagtgaattcagcagtaagc                       >  1:212279‑M2/1‑35 (MQ=255)
                                                                                                                    aTGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTTtaaatgtaaa        <  2:39103/149‑1 (MQ=255)
                                                                                                                        ttatgAGTTGCACTAAggaaggtgctaacaagtccctgatatgagatcatgtttgtcatctggagccatagaatagggttcatcatgagtcatcaacttaccttagcccacagtgaattcagcagtaagcgccctcagaccagaaaaga    >  1:244214‑M2/2‑16 (MQ=255)
                                                                                                                        atatGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCCGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCCCGTGTTTGTATAAATGTAAAtgtg    >  2:115484/1‑149 (MQ=255)
                                                                                                                          cgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAg  >  2:309512‑M2/11‑149 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAG  >  CP009273/2400125‑2400395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.