Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 3,178,254 IS5 (+) +4 bp 48.8% coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)11 (0.570) 10/274 NT 49.3% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 3178254 = 11 (0.590)coding (56/291 nt) yqiC BMFP family putative fusogenic protein
* ? CP009273 = 2283592NA (NA)10 (0.520) 10/276 NT 46.9% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 3178257 11 (0.590)coding (59/291 nt) yqiC BMFP family putative fusogenic protein

AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCA  >  CP009273/2283452‑2283738
                                                                                                                                            |                                                                                                                                                  
aGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCt                                                                                                                                            <  1:273304/149‑1 (MQ=35)
   aactctcTTTTCTGGTCTGACGGCGCTTACTGCTGTATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACATACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACACTGTa                                                                                                                                         <  1:180184/146‑1 (MQ=255)
   aaaTCTCTTTGCTGGTCTGACGGCGCTTACGGCTGAAGTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                         <  2:18565/149‑1 (MQ=255)
                 gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCAGTAGCCACCAGAACAGa                                                                                                                           >  2:211392/1‑149 (MQ=255)
                                  gctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                          <  1:144622/149‑1 (MQ=255)
                                  gctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGAAGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCACCCATGACAGAGATTGCGGAACGACaac                                                                                                          >  1:20422/1‑148 (MQ=17)
                                   ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACAACCAag                                                                                                         >  2:62893/1‑149 (MQ=255)
                                    tgAATTCACGGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCATGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCATTTTTCATAACGATGTAGCCACCAGAACAGAGATTGCGGAACGACAAaga                                                                                                        <  1:266914/149‑1 (MQ=18)
                                             tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGaa                                                                                               <  1:289431/149‑1 (MQ=255)
                                                                       atgatgACCCCTGTTCTATGTCTCCAGATGACAAACATGACCGCATAGCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATc                                                                     <  2:43750/149‑1 (MQ=37)
                                                                         gattaACCCTTTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGTGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCt                                                                   <  2:168881/149‑1 (MQ=255)
                                                                                          ggttCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGt                                                  <  2:266133/146‑1 (MQ=255)
                                                                                                         aCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTa                                   <  2:320986/149‑1 (MQ=255)
                                                                                                                      tCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcaca                      >  2:62307/1‑149 (MQ=255)
                                                                                                                            aCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGccc                >  2:263975/1‑147 (MQ=255)
                                                                                                                                 ttagCACCTTCCATAACGCTGTAGCCCCCAGAACAGATATTGCGGAACGACAACAAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTCCTGTTAAAAGCATTGGGTACAGAAAATAACCATAGCTCCAtca           >  2:88159/4‑147 (MQ=255)
                                                                                                                                      aCCTTCCATACCGCCGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAACCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa      <  2:67301/149‑1 (MQ=255)
                                                                                                                                          tCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCa  <  1:62307/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCA  >  CP009273/2283452‑2283738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.