Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 3,178,254 IS5 (+) +4 bp 48.8% coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)11 (0.570) 10/274 NT 49.3% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 3178254 = 11 (0.590)coding (56/291 nt) yqiC BMFP family putative fusogenic protein
* ? CP009273 = 2283592NA (NA)10 (0.520) 10/276 NT 46.9% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 3178257 11 (0.590)coding (59/291 nt) yqiC BMFP family putative fusogenic protein

GCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCT  >  CP009273/3178106‑3178401
                                                                                                                                                    |                                                                                                                                                    
aaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccc                                                                                                                                                      >  1:310124‑M2/149‑149 (MQ=255)
    atgaggcaggacgctttaaagggcggagaaacggtctaaataggctgagtcaaggcatttacgtgagaaaacatcggctcaaacatgaagaaatgcactgacttagtccgccgagaagaatttccccgcttattcgcaccttccCTaaa                                                                                                                                                  <  2:174905‑M2/5‑1 (MQ=255)
     ctggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAg                                                                                                                                                 <  2:247034‑M2/6‑1 (MQ=255)
            taacgccttaaatggcgaagaaacggtctaaatagccacattcaagacatatacaagagaaaaaataagctaaaacatgaagaaatgaaatgactgagtcaaccgagaagaatttccccgcttattcgcaccttccCTAAAGGTATCAg                                                                                                                                         >  1:13941‑M2/137‑149 (MQ=255)
               cgccttaaatggcgaagaaacgcgctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgatatgactgtgtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGa                                                                                                                                      <  1:86955‑M2/16‑1 (MQ=255)
                      aaTTCTGGTTGATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGGACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCACGTCCCCGAATCAATGCCTAAAGGAATCAGGGAGTTCggg                                                                                                                               <  2:234374/149‑1 (MQ=255)
                       atggcgaagaaacggtttaaataggctgattcaaggcatttacgtgagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCgggg                                                                                                                              <  2:9374‑M2/24‑1 (MQ=255)
                           tGGTTTATGACTTACCCTTCTCGCACTACACTGGCACTCAACACCTATCAGTACAGAAAAAATACAATGATTAACCCGAAAAAAATTGAGCCAATCGCTCGCCAGGTTCACGAATCAATGCCTAAggaaggtgcgaacaagtcccggat                                                                                                                           <  1:104481‑M2/149‑25 (MQ=255)
                               ttATGACTTACCCTTATCGCCCTACAATGGCACTCACCACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCTAGGTTCACGAATCAATGCCTAAggaaggtgcgaacaagtccctgatatga                                                                                                                       >  2:253599‑M2/1‑121 (MQ=255)
                                      ttACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAggaaggtgcgaacaagtccctgatatgaaatcatg                                                                                                                <  2:119741‑M2/149‑36 (MQ=255)
                                        aCCCTTATCGCACTACAATGACACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACACGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGaaaaaa                                                                                                             >  1:287387/1‑149 (MQ=255)
                                         cccTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACGATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAggaaggtgcgaacaagtgcctgctatgagatcatgttt                                                                                                             <  2:112783‑M2/149‑39 (MQ=255)
                                         cccTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCATGAATCAATGCCTAAggaaggtgcgaacaaggccctgatatgagatcatgttt                                                                                                             >  1:24693‑M2/1‑111 (MQ=255)
                                         aaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGaaaaaaa                                                                                                            <  1:267424‑M2/42‑1 (MQ=255)
                                          ccTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttg                                                                                                            >  2:55941‑M2/1‑110 (MQ=255)
                                          ccTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGAACCGCACAAACTTGAGCAAATCGCTCGCCAGGTGCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAAt                                                                                                           >  2:157925/1‑149 (MQ=255)
                                             aggctgaatcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCg                                                                                                        <  2:13684‑M2/46‑1 (MQ=255)
                                                           ggCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTAAGCAAATCGCTCGCCAGGTTCACGAATAAATGTTACTGTCAAACAGGGAGTTCCGGGAACATGTGGAGAAAAAAATCCGCCAAACCCTACAAg                                                                                          >  1:153857/1‑149 (MQ=255)
                                                              ttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcacctttcTTATTGGATTCAGTGAGTTTGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAgcgc                                                                                       <  1:170569‑M2/63‑1 (MQ=255)
                                                                 cgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGc                                                                                    <  2:117174‑M2/66‑1 (MQ=255)
                                                                         aaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACgcgc                                                                            <  2:231624‑M2/74‑1 (MQ=255)
                                                                           cAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATAAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCt                                                                          >  2:120518/1‑149 (MQ=255)
                                                                                 aGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCt                                                                    >  1:188161/1‑149 (MQ=255)
                                                                                     aatCTGCATTGTTTGTCCCGAATAAAATTGAGCAAATCGCTCGCCAGGTTCACGGATCAATGCCTTAggaaggtgcgtacaagttcctgatatgagttcttgtttgtcatctggagccatagaacagggttcatcatgagtcatcaact                                                                 >  2:239851‑M2/1‑67 (MQ=255)
                                                                                            aaTGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaagttaccttc                                                          <  2:161595‑M2/149‑90 (MQ=255)
                                                                                                        gAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagtcatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaa                                              >  1:115072‑M2/1‑48 (MQ=255)
                                                                                                              aaTTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCaaa                                       <  2:188161/149‑1 (MQ=255)
                                                                                                                    gagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGGGTTCGACGTCCAAACGCAAg                                 >  2:47791‑M2/33‑149 (MQ=255)
                                                                                                                     cAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGt                                >  2:25996/1‑149 (MQ=255)
                                                                                                                              cgcCAGGTTCACGAATCAATGCCTAAggaggcttctaacaagtccatgctatgagatcatgtttgtcatctggagtcatagaacagggttcatcatgagttattaacttaccttcgccgacagtgaattcagcagtaagcgccgtcaga                        >  1:119741‑M2/1‑26 (MQ=255)
                                                                                                                                     ttCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGCCGTCCAACCGCAAGTCCTGTTACGGACTCGt                >  2:156664/1‑149 (MQ=255)
                                                                                                                                     ttCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGt                >  1:32568/1‑149 (MQ=255)
                                                                                                                                       attcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGa              <  2:269107‑M2/136‑1 (MQ=255)
                                                                                                                                         cGAAGCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTTCAAGCGCAGCTGTCGCGCCTCGACCTTTTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGGCCTGTTACGGACTCGTGaaa            <  1:50522/149‑1 (MQ=255)
                                                                                                                                                   ccTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagacattttctggg   <  1:132120‑M2/149‑145 (MQ=255)
                                                                                                                                                    cTAAggaaggtgcgaacaagtccctgatatgacatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagtgccgtcagaccagaaaagagattttcttgtc  >  2:147948‑M2/1‑4 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCT  >  CP009273/3178106‑3178401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.