Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,215,619 IS2 (+) +5 bp 33.3% intergenic (‑151/‑41) bglX ← / → dld beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2062422 =NA (NA)6 (0.260) 6/268 NT 39.1% noncoding (1331/1331 nt) IS2 repeat region
?CP009273 2215619 = 9 (0.400)intergenic (‑151/‑45) bglX/dld beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent
* ? CP009273 = 2063752NA (NA)3 (0.130) 3/268 NT 24.3% noncoding (1/1331 nt) IS2 repeat region
?CP009273 = 2215623 9 (0.400)intergenic (‑155/‑41) bglX/dld beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent

GTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATT  >  CP009273/2063618‑2063893
                                                                                                                                      |                                                                                                                                             
gtgGTACGGCGTCTGCGTTTCTCCGGCCCTAAGGCATCAATCACCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGc                                                                                                                                     <  2:414666/145‑1 (MQ=255)
    tACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCAtcc                                                                                                                                 >  1:219737/1‑145 (MQ=39)
     aCGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCAtcct                                                                                                                                <  1:186831/145‑1 (MQ=40)
     aCGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTCGCGAAGCAtcct                                                                                                                                >  1:284046/1‑145 (MQ=38)
           tCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGGCTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGAGACAGTCTTGTGGCGCAGCCTCGACCCgtg                                                                                                                          >  1:243075/1‑145 (MQ=11)
               cGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATGGGCGAAGCATCCTCCCGTGtttt                                                                                                                      >  1:286980/1‑145 (MQ=255)
               cGTTTCTCCGGCCCTAAGACATCAAGCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGtttt                                                                                                                      <  2:368073/145‑1 (MQ=255)
                    ctcCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATtc                                                                                                                 >  1:380079/1‑145 (MQ=255)
                                    tcaattaTCTGTTCTCCAATGACTAGTCCGACTATTAGTATTAAGACTATCCCTTATTTAAGTGATATTGGTTTTCTGGAGATTCACGGGGCCAGTCTAGTGTCGACGCATCCTCCCTGGTTTTAATTCTCCTTGGTGGCCagga                                                                                                 >  1:197030/1‑145 (MQ=17)
                                                tctcCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCa                                                                                     <  1:62074/145‑1 (MQ=255)
                                                    caatcagcaGTCTAAAAACTAGAATTAAGACTATCACTTATTTAAGCGATATTGGTTGTCTGGAGATTCAGGAAGCCAGACTAGTGACGAAGCAGCCTCACGAGTCTTAATTATCATAGATGGTCAGGAGATAACTTATCAgcgc                                                                                 >  1:368411/9‑145 (MQ=18)
                                                              tCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATa                                                                       >  1:355732/1‑145 (MQ=255)
                                                                tAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATCCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAAt                                                                     <  2:60877/145‑1 (MQ=255)
                                                                          tATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGAGGGCCAGTCTAGTGGCGAAGCAGCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAAATTATCAGCGCGCCGGAGATAAATaagagatgg                                                           >  2:362070/1‑137 (MQ=25)
                                                                                   tATCACTTAGTTAATGGATATTGGTTCTGTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCTTCCTCCCTTGTTTTAATTCTCAGTGATGGTCAGGAGGTTACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGa                                                  <  1:203637/145‑1 (MQ=38)
                                                                                     tCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATa                                                <  1:383716/145‑1 (MQ=255)
                                                                                         ttATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGGTAATTATGGTGTGGGACTGTTGATAGATg                                            >  2:192709/1‑145 (MQ=255)
                                                                                           aTTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAg                                          >  1:302210/1‑145 (MQ=255)
                                                                                                       ttGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGCTAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAg                              >  1:31604/1‑145 (MQ=255)
                                                                                                                tGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCGTTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAg                     <  2:219737/145‑1 (MQ=255)
                                                                                                                         aGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGaaa            >  1:391774/1‑145 (MQ=255)
                                                                                                                            gggCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGt         >  2:174970/1‑145 (MQ=255)
                                                                                                                                   tCTAGTGGCGAAGCATCCTCCCGAGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGGCCATAtt  >  2:234786/1‑145 (MQ=255)
                                                                                                                                      |                                                                                                                                             
GTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATT  >  CP009273/2063618‑2063893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.