breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 444,779 C→T W440* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 1,138,538 T→G E429A (GAA→GCA)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
RA 1,191,676 C→T H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,688 C→T T370T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,563,861 G→T L296M (CTG→ATG)  gadC ← glutamate:gamma‑aminobutyric acid antiporter
RA 2,305,991 G→T Q80K (CAG→AAG)  ompC ← outer membrane porin protein C
JC 3,528,829 (GTTTTCAGCCAG)1→2 coding (399/1353 nt) envZ ← sensory histidine kinase in two‑component regulatory system with OmpR
JC 3,676,833 Δ3 bp intergenic (‑26/+155) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
RA 3,919,680 C→T A46T (GCC→ACC)  mioC ← FMN‑binding protein MioC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 1116965 1118123 1159 64 [62] [61] 63 pyrC pyrC
* * ÷ CP009273 1191701 1206927 15227 65 [60] [62] 63 [icd]–[icdC] 25 genes
[icd], ymfD, ymfE, lit, intE, xisE, ymfI, ymfJ, cohE, croE, ymfL, ymfM, oweE, aaaE, ymfR, beeE, jayE, ymfQ, stfP, tfaP, tfaE, stfE, pinE, mcrA, [icdC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)218 (1.850) 135/292 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1045234 =135 (1.150)99 (0.840) 75/292 0.6 58.2% intergenic (‑34/‑55) gfcA/insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
?CP009273 = 1973123 7 (0.060)intergenic (‑669/‑111) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 = 1046001114 (0.970)134 (1.140) 92/292 0.1 69.1% intergenic (+15/+418) insB1/cspH IS1 transposase B/stress protein, member of the CspA‑family
?CP009273 1973115 = 6 (0.050)intergenic (‑661/‑119) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 = 3180785NA (NA)151 (1.280) 99/292 0.0 93.2% pseudogene (6/2445 nt) yqiG pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
?CP009273 = 4334511 11 (0.090)coding (1326/1422 nt) melB melibiose:sodium symporter
* ? CP009273 4334507 =4 (0.030)147 (1.250) 100/292 0.0 97.4% coding (1322/1422 nt) melB melibiose:sodium symporter
?CP009273 4487998 = NA (NA)intergenic (+241/‑91) intB/insC1 pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase/IS2 repressor TnpA