breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
JC 102,158 +TGGTAATAT 5.1% coding (1394/1476 nt) murC → UDP‑N‑acetylmuramate:L‑alanine ligase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 526,792 A→C 6.6% S1178R (AGC→CGC)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,796 C→G 6.7% A1179G (GCG→GGG)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 872,805 C→T 7.6% intergenic (+4/‑174) gsiD → / → yliE glutathione ABC transporter permease/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 1,529,175 T→C 8.5% pseudogene (1274/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,180 T→C 8.3% pseudogene (1279/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,185 C→T 8.6% pseudogene (1284/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,203 C→A 12.7% pseudogene (1302/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,218 C→T 16.9% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,708,758 G→C 9.7% E614Q (GAA→CAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,054,976 A→G 5.5% intergenic (+17/‑85) shiA → / → amn shikimate transporter/AMP nucleosidase
RA 2,054,977 T→G 5.3% intergenic (+18/‑84) shiA → / → amn shikimate transporter/AMP nucleosidase
RA 2,054,978 C→A 5.2% intergenic (+19/‑83) shiA → / → amn shikimate transporter/AMP nucleosidase
RA 2,054,979 C→T 5.2% intergenic (+20/‑82) shiA → / → amn shikimate transporter/AMP nucleosidase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,505,145 T→C 5.7% R292R (AGA→AGG yhfS ← FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,809 Δ1 bp 6.7% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,815,822 T→G 6.7% intergenic (‑54/+12) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,815,937 C→A 7.3% pseudogene (613/669 nt) rph ← ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 4,184,809 G→T 64.7% G1189C (GGT→TGT)  rpoB → RNA polymerase, beta subunit
RA 4,296,190 A→G 20.6% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 23.6% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 10.4% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,566,266 T→C 5.3% R327R (CGA→CGG yjiN ← zinc‑type alcohol dehydrogenase‑like protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =84 (0.740)40 (0.380) 31/462 NT 34.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 72 (0.680)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780584 (0.740)55 (0.520) 39/462 NT 42.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 72 (0.680)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)38 (0.340) 34/486 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2054966 =113 (1.000)6 (0.060) 5/474 NT 5.5% intergenic (+7/‑95) shiA/amn shikimate transporter/AMP nucleosidase
?NC_000913 2054990 = 99 (0.910)intergenic (+31/‑71) shiA/amn shikimate transporter/AMP nucleosidase
* ? NC_000913 = 2515616NA (NA)7 (0.060) 7/488 NT 5.4% noncoding (1344/1345 nt) IS186 repeat region
?NC_000913 4543608 = 122 (1.090)coding (494/549 nt) fimA major type 1 subunit fimbrin (pilin)