breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 129,798 | A→T | 9.1% | I488N (ATC→AAC) | yacH ← | DUF3300 family protein |
RA | 238,472 | C→G | 7.1% | A89P (GCC→CCC) | ykfM ← | lethality reduction protein, putative inner membrane protein |
MC JC | 257,908 | Δ776 bp | 100% | [crl] | [crl] | |
RA | 349,859 | C→G | 5.0% | intergenic (+287/‑153) | prpB → / → prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
RA | 1,466,210 | G→A | 6.1% | pseudogene (819/2513 nt) | ydbA → | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
RA | 1,529,175 | T→C | 8.5% | pseudogene (1274/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,180 | T→C | 8.2% | pseudogene (1279/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,185 | C→T | 8.1% | pseudogene (1284/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,203 | C→A | 10.8% | pseudogene (1302/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,218 | C→T | 21.5% | pseudogene (1317/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,616,197 | C→A | 5.8% | V145V (GTC→GTA) | yneK → | uncharacterized protein |
MC JC | 1,978,503 | Δ776 bp | 100% | insB1–insA | insB1, insA | |
RA | 2,173,361 | Δ2 bp | 100% | pseudogene (1‑2/442 nt) | gatC ← | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 3,103,900 | T→C | 5.7% | H296H (CAT→CAC) | mutY → | adenine DNA glycosylase |
RA | 3,253,930 | C→A | 5.6% | W95C (TGG→TGT) | yhaJ ← | LysR family putative transcriptional regulator |
RA | 3,560,455:1 | +G | 93.8% | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,688,287 | A→C | 15.0% | V296G (GTG→GGG) | bcsC ← | cellulose synthase subunit |
RA | 3,815,809 | Δ1 bp | 6.1% | intergenic (‑41/+25) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
RA | 3,815,822 | T→G | 9.4% | intergenic (‑54/+12) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
JC | 3,815,859 | Δ82 bp | 10.9% | [rph]–[rph] | [rph], [rph] | |
RA | 4,184,809 | G→T | 41.4% | G1189C (GGT→TGT) | rpoB → | RNA polymerase, beta subunit |
RA | 4,296,060 | C→T | 23.2% | intergenic (+266/+376) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,296,190 | A→G | 29.2% | intergenic (+396/+246) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,296,191 | A→C | 25.5% | intergenic (+397/+245) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,296,380:1 | +CG | 100% | intergenic (+586/+56) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 82 (0.720) | 32 (0.300) | 27/458 | NT | 30.0% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 73 (0.690) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 82 (0.720) | 52 (0.490) | 39/458 | NT | 41.0% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 73 (0.690) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 68 (0.610) | 58/482 | NT | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | = 4542690 | 19 (0.170) | 3 (0.030) | 3/472 | NT | 6.2% | intergenic (+57/‑425) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |
? | NC_000913 | = 4542986 | 73 (0.670) | intergenic (+353/‑129) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |