breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 129,798 A→T 9.1% I488N (ATC→AAC)  yacH ← DUF3300 family protein
RA 238,472 C→G 7.1% A89P (GCC→CCC)  ykfM ← lethality reduction protein, putative inner membrane protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 349,859 C→G 5.0% intergenic (+287/‑153) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 1,466,210 G→A 6.1% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,529,175 T→C 8.5% pseudogene (1274/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,180 T→C 8.2% pseudogene (1279/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,185 C→T 8.1% pseudogene (1284/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,203 C→A 10.8% pseudogene (1302/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,218 C→T 21.5% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,616,197 C→A 5.8% V145V (GTC→GTA yneK → uncharacterized protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,103,900 T→C 5.7% H296H (CAT→CAC mutY → adenine DNA glycosylase
RA 3,253,930 C→A 5.6% W95C (TGG→TGT yhaJ ← LysR family putative transcriptional regulator
RA 3,560,455:1 +G 93.8% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,688,287 A→C 15.0% V296G (GTG→GGG)  bcsC ← cellulose synthase subunit
RA 3,815,809 Δ1 bp 6.1% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,815,822 T→G 9.4% intergenic (‑54/+12) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
JC 3,815,859 Δ82 bp 10.9% [rph][rph] [rph], [rph]
RA 4,184,809 G→T 41.4% G1189C (GGT→TGT)  rpoB → RNA polymerase, beta subunit
RA 4,296,060 C→T 23.2% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→G 29.2% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 25.5% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =82 (0.720)32 (0.300) 27/458 NT 30.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 73 (0.690)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780582 (0.720)52 (0.490) 39/458 NT 41.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 73 (0.690)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)68 (0.610) 58/482 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 454269019 (0.170)3 (0.030) 3/472 NT 6.2% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 73 (0.670)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)