Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,606,148 IS2 (+) +5 bp coding (49‑53/876 nt) lsrF → putative autoinducer‑2 (AI‑2) aldolase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1467910 =NA (NA)90 (0.880) 53/470 0.6 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 1606148 = 0 (0.000)coding (49/876 nt) lsrF putative autoinducer‑2 (AI‑2) aldolase
* ? NC_000913 = 1469240NA (NA)76 (0.740) 47/470 0.8 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 1606152 0 (0.000)coding (53/876 nt) lsrF putative autoinducer‑2 (AI‑2) aldolase

TTGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACT  >  NC_000913/1468994‑1469489
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
ttGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAAcc                                                                                                                                                                                                                                                       <  1:1734/251‑1 (MQ=11)
  gCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGAACGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGCCTAGTCTCAAAACTAGTATTAAGACTAACACTTATTTAAGTAATATTGGTTGCCTGGAGATTCAGGGGGCCAGTTTAAAcccc                                                                                                                                                                                                                                                     >  2:695983/1‑251 (MQ=9)
     tACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGa                                                                                                                                                                                                                                                  >  1:50161/1‑251 (MQ=32)
                aaTAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATg                                                                                                                                                                                                                                       >  1:858940/1‑251 (MQ=37)
                  tAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCt                                                                                                                                                                                                                                     >  1:1094962/1‑251 (MQ=38)
                          tggctgCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTACGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCgg                                                                                                                                                                                                                             >  2:1072499/1‑251 (MQ=255)
                                  acacCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGaaaa                                                                                                                                                                                                                     <  2:50202/251‑1 (MQ=255)
                                     ccATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTg                                                                                                                                                                                                                  >  2:985934/1‑251 (MQ=255)
                                      cATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTCTCCTGTGTGGTACGCCGTATCCGTTTCTCCGGCCCTAAGAAATCAATCATCTGTTCCACAATGATTAGTACAAACACTAGTATTAAGACTATTACTTATTTAAGTGATATTGGTTGTCTGGCAATTCAGGGGGCAAGTCTAAACCACAGAACTTACTGAGTCCGATTCCGGCGCGAAAATTGt                                                                                                                                                                                                                 >  2:512507/1‑251 (MQ=14)
                                            ggcGGGCAACTAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCGATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTAATTAAGAGATATTGGTTGTCTGGAGATGCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTtaataa                                                                                                                                                                                                           <  2:738183/249‑1 (MQ=255)
                                                             aCCGTCATCCCCGGTCCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCa                                                                                                                                                                                          <  1:1072337/251‑1 (MQ=255)
                                                              ccGTCATCCCCAGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCaa                                                                                                                                                                                         <  1:893923/251‑1 (MQ=255)
                                                                                 gCTCTGCTGCACAATTGGGTTATTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTATGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCCAGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAAATGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTa                                                                                                                                                                      <  2:601684/251‑1 (MQ=255)
                                                                                        tgAACGATTGCTATCTTTTCCTGTGTGGTACGCGGTCTGCGTTACTCCTGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGCATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGaaa                                                                                                                                                               <  1:333459/251‑1 (MQ=255)
                                                                                        tgAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGaaa                                                                                                                                                               <  1:985772/251‑1 (MQ=255)
                                                                                                  cGATCTTTCCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAAcccc                                                                                                                                                                                                                                                     >  2:864426/1‑155 (MQ=14)
                                                                                                              gtgtgGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACCAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGtt                                                                                                                                                                                                                <  2:213179/180‑1 (MQ=255)
                                                                                                               tgtgGTACGCCTTCTTCGTTTCTCCGGCCCTAAGACATCCATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGAAGATTCAGGGGGCCTGTCTAATCCCCAGAACTTACTTATGCGGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGtttttt                                                                                                                                        <  1:755648/251‑1 (MQ=255)
                                                                                                                    tACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATg                                                                                                                                   <  2:1095129/251‑1 (MQ=255)
                                                                                                                              gCGTTTCTCCGGCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTGTTGAATGAGACGAACAtt                                                                                                                        >  1:1058842/1‑251 (MQ=255)
                                                                                                                               cGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAACGACTAGTCTAAAAACTAGTATTAAGACTATCACTTAATTAAGTGATATTGGTTGTCTGGGGATTCAAGGTGCCTGTCTAAACCCCAGAACTTACTGATGCTGATTCCAGTGCGAAAATTGTTAATAAAGGTACTTGTAGAGTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACAtt                                                                                                                        <  1:695858/251‑1 (MQ=37)
                                                                                                                                      ccGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACCAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAAt                                                                                                                                                                                        >  1:213129/1‑180 (MQ=255)
                                                                                                                                       cagCCCTGAGACTTCGATCATCAGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATAATGGTTGTCTTGAGATTCAGAGGGACAGTCTAAACCCCAGAACTTACTTATTCTGAGTCCGGTGCGAAAATTGTGTAAAAAGGTACTTGTTTGTTCAATTGGTCAACGCAAGAGTTATGTGAATACATGTGGTTGCGGATGTTTTTTGACTGACACGAAGATTTAcagcag                                                                                                                <  2:1073569/249‑1 (MQ=21)
                                                                                                                                       cGGCCCTAAGACATCAATCATCTGTTCTCCAATTACTAGTCTAAAAACTAGTATTAAGACTATCTCTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTTTGCTGATTCCGGTGCGAAAAGTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAGCATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagcag                                                                                                                <  1:663573/251‑1 (MQ=255)
                                                                                                                                                 agctccattctcTGTTCTTCAATGGACAGTCTTAAAGCTAGTATTAATACTATCACTTATTTAAGTGATATTTGTTGTCTTGAGGTTCAGGGGGCCAGTCTAAACCCCATAACGTTCTTATGCTGATTCCGGTGCGAATATTGTTAATAAAGGTACTTGTTGTTTCAATTTGTCAACGCAACAGTTATGTGAAAACATGGGATTTCGGAGGTTTTTTGAATGAGACGAACATTTACATCAGAGGAAAAAGc                                                                                                      <  2:668683/241‑1 (MQ=25)
                                                                                                                                                                tctcCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACCTCTCAAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCCAAACCCCAGAACTTACTTATGCTGATACCGGTGCGAAAATTTTAAATAAAGGTATATGTAGTTTCAATTGGTCAACGCATCAGTTATGTGAAAACATGGGGTTGCGGAGGTCTACTGAATGAGTCGATCACTTACAGAAGAGGAAAAAGACTCTGTTTTTGAACt                                                                                       >  1:340918/1‑251 (MQ=25)
                                                                                                                                                                            aGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTTCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCATAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACgg                                                                           >  1:1050461/1‑251 (MQ=255)
                                                                                                                                                                                      cTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTc                                                                 >  1:589784/1‑251 (MQ=255)
                                                                                                                                                                                      cTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTc                                                                 >  1:1036119/1‑251 (MQ=255)
                                                                                                                                                                                         gTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGtttttt                                                                                                                                        <  1:831375/177‑1 (MQ=255)
                                                                                                                                                                                              aaggatATCATTTATTTAAGTGATATTGGTTGTCTGGAGATTCATGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGTTATTTGTAGATTCAATTGGTTAACGCAACAGTTATATGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAAt                                                         <  2:1059004/246‑1 (MQ=255)
                                                                                                                                                                                                gACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGAtt                                                                                                                                                                                            <  1:925029/118‑1 (MQ=255)
                                                                                                                                                                                                gACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAg                                                       >  2:683298/1‑251 (MQ=255)
                                                                                                                                                                                                     tCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAat                                                  >  1:824860/1‑251 (MQ=255)
                                                                                                                                                                                                        cTTATTTCAGAGATATTGGTTGTCTGGGGATTCATGAGGCCAGTCTAAAACCCAGAACTTACTTATTGTGGATCCGGGACGGAAATTGTGAGTAAAGGGACTTGTAGATTCAATTGGTCAACGCAACAGATATGTGAGAACGTGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTa                                                                                      <  2:120259/212‑1 (MQ=18)
                                                                                                                                                                                                           aTTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTg                                            <  2:124118/251‑1 (MQ=255)
                                                                                                                                                                                                           aTTTAAGTGATAATGGTTGTATGGAGATTCAGGGCACCAGTCTAAAACCCAGGAATTACATATGCTGATTGCGGTGCGAAAATTGTTGATAAGGGTACTTGTAGATACAATTGGTCAACGCAACAGTTATATGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGa                                                                                                                             <  1:1027078/170‑1 (MQ=25)
                                                                                                                                                                                                             ttAAGTGATATTGGTTGTCTGGAGATTCAGGAGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAAGTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTggg                                          <  2:1036282/251‑1 (MQ=255)
                                                                                                                                                                                                               aaGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGtt                                        <  1:683172/251‑1 (MQ=255)
                                                                                                                                                                                                                 gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagcag                                                                                                                >  2:831521/1‑177 (MQ=255)
                                                                                                                                                                                                                   gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCaaa                                    >  1:124075/1‑251 (MQ=255)
                                                                                                                                                                                                                   gATATTGGTTGTCTGGAGATTCAGGGCGCCAGTCTACACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAACTGTTAATACAGGTACTTGTATATCCACTTGTTCAACGCAACAGTTTTGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGACACCAACGTTTACAACCGAGGAAAACGCCTCTGTTTTTGACCGATGGAAGAACGGAACAGGCTTAACTCACATCACGAATATCCTAGGTACaca                                    >  2:1062457/1‑249 (MQ=21)
                                                                                                                                                                                                                    atatTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGaaa                                                                                                                                                               <  2:705901/127‑1 (MQ=255)
                                                                                                                                                                                                                      atTGGTTGTCTTGAGATTCAGGGGGCCAGTCGAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTAATTGTAGATTCAATTAGTCAACGCAACAGTTATGTGAAAACGTGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAAcc                                 <  1:37985/251‑1 (MQ=255)
                                                                                                                                                                                                                        tGGTTGTCTGGGGATTCGGGGGGCCAGTCTAAACCCCTGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTAAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCg                               <  1:576747/251‑1 (MQ=255)
                                                                                                                                                                                                                        tGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTTAACGCAACAGTTAtg                                                                                                                                                                    >  2:925181/1‑118 (MQ=255)
                                                                                                                                                                                                                        tGGTTGTATGGAGCTGCAGGGTGCCAGTCTAACCCCCAGAACTTACTTATTCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGAATCAATTGTTTAACGCAACAGTTATGCGAAAACATCGGTTTGCGGATGTTTTTCAAATGAGACAAACATTTACAGAATAGGTAAAAGCCTCTGTCTTTTTAATACAGAAAAACGGAACAGCGATAAGTGAAATATCGAATATCCTGTGCTAaaaaactg                               >  2:762194/1‑247 (MQ=255)
                                                                                                                                                                                                                            tGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAAc                           >  1:1011069/1‑251 (MQ=255)
                                                                                                                                                                                                                              tCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcag                                                                                                                   <  2:413214/161‑1 (MQ=255)
                                                                                                                                                                                                                                tGGAGATTCAGGGGGCTAGTCTAAACCCCAGTACTTACTTATGCTGATTCCGGTGCGAAACTTGTTAATAAAGGTACTTGTAGATTTAATTGGTCAACTCAACAGTTATGTGAAAACATGGGGTAGCGGAGGTTTTTTGAAAGAGACGAACATTTACAGCAGAGGTAAATGCTGCTGTTTGTGAACTAGGGAATAATGGAACAGGCCTTAGTGAAATAGCAAATATCCTGGGTTCAAAAACCGGAACAATc                       >  2:38026/1‑251 (MQ=255)
                                                                                                                                                                                                                                tGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAAGGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGTTTCAAAACCCGGAACGAta                       >  2:871243/1‑250 (MQ=255)
                                                                                                                                                                                                                                  tggtttaaGGGGGCCAGTCTAATCCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTTTTAGTAGAGGTACTTTTAGATTCAATTGGTCAACGGCAAAATTATGTTAGAACATAGGATTGCGTAGGTTTTTTGAATGAGACGAACAGTTACATCCGAAGAAAAAACCTCTGTTTTTGAACTATGGAAGAACTGAACAGGCTTCAGTGAAATAGCGAATATCCTAGGTTCAAAACCCGGAACGATCtt                     <  2:528382/244‑1 (MQ=17)
                                                                                                                                                                                                                                         aGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACACGGAACGATCTTCACTATg              >  1:454975/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gggggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCATAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCCCTATGt             <  1:512394/251‑1 (MQ=255)
                                                                                                                                                                                                                                           ggggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTCGAAGTATGGAAGAACGGAACAGGCTTCAGTGAAAAAGCGAATATCCTGGGCTCaaaatctgtctcttaaaccaatctccg            >  1:546117/1‑228 (MQ=18)
                                                                                                                                                                                                                                           ggggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTGGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGATTCAGTGAAATAGAGAATATCCTGGGTTCAAAACACGGAACGATTTTCACTATGtt            >  2:576863/1‑251 (MQ=255)
                                                                                                                                                                                                                                            gggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTg                                                                                                                                       >  1:705772/1‑127 (MQ=255)
                                                                                                                                                                                                                                            gggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTa           >  1:386599/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cAGTCTAAACCCCAGGACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAAAATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAggg       <  1:871094/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  gTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATgggg                                                                                                                                                       <  2:395916/105‑1 (MQ=255)
                                                                                                                                                                                                                                                  gTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCaaa                                    <  1:945643/220‑1 (MQ=255)
                                                                                                                                                                                                                                                     tAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTATAGCAGAGGAAAACGCCTATGTTTTTGAACTATGGATGAACGGAACAGGCCTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGTACGATCTTCACTTCGTTAAGGGATACt  >  1:528269/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      aaaCCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTg                                                                                           >  1:413123/1‑161 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
TTGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACT  >  NC_000913/1468994‑1469489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.