New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1543030 =45 (1.080)6 (0.150) 5/244 4.2 13.2% coding (1031/1389 nt) narU nitrate/nitrite transporter
?NC_000913 1543053 = 36 (0.910)coding (1008/1389 nt) narU nitrate/nitrite transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCGATTTTCAGTGCCCTGCTGTTCCTTACCTTACCGGGCACAGGCTCCGGTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1543083‑1543030
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccggtaaGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACC  >  NC_000913/1543053‑1543142
                                                                                                                                                
GGCGATTTTCAGTGCCCTGCTGTTCCTTACCTTACCGGGCACAGGCTCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACC  <  7:160929/144‑1
GGCGATTTTCAGTGCCCTGCTGTTCCTTACCTTACCGGGCACAGGCTCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACC  >  8:160929/1‑144
                             CCTTACCGGGCACAGGCTCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAA         >  1:173733/1‑108
                             CCTTACCGGGCACAGGCTCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAA         <  2:173733/108‑1
                             CCTTACCGGGCACAGGCTCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAAC                                        >  5:193367/1‑77
                             CCTTACCGGGCACAGGCTCCGGTAAGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAAC                                        <  6:193367/77‑1
                                                                                                                                                
GGCGATTTTCAGTGCCCTGCTGTTCCTTACCTTACCGGGCACAGGCTCCGGTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1543083‑1543030
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccggtaaGGTAAGGAACAGCAGGGCACTGAAAATCGCCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACC  >  NC_000913/1543053‑1543142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.