mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-1-BOP-1000-ACNB-END-3_S10_L001_R1_001.good.fq | 425,710 | 60,444,676 | 100.0% | 142.0 bases | 149 bases | 98.2% |
| errors | Plate-1-BOP-1000-ACNB-END-3_S10_L001_R2_001.good.fq | 425,710 | 60,280,584 | 100.0% | 141.6 bases | 149 bases | 98.0% |
| errors | Plate-1-BOP-1000-ACNB-END-3_S10_L002_R1_001.good.fq | 392,758 | 55,815,852 | 100.0% | 142.1 bases | 149 bases | 98.3% |
| errors | Plate-1-BOP-1000-ACNB-END-3_S10_L002_R2_001.good.fq | 392,758 | 55,682,246 | 100.0% | 141.8 bases | 149 bases | 97.6% |
| errors | Plate-1-BOP-1000-ACNB-END-3_S10_L003_R1_001.good.fq | 209,585 | 29,759,125 | 100.0% | 142.0 bases | 149 bases | 98.1% |
| errors | Plate-1-BOP-1000-ACNB-END-3_S10_L003_R2_001.good.fq | 209,585 | 29,673,853 | 100.0% | 141.6 bases | 149 bases | 98.1% |
| errors | Plate-1-BOP-1000-ACNB-END-3_S10_L004_R1_001.good.fq | 419,096 | 59,453,809 | 100.0% | 141.9 bases | 149 bases | 98.3% |
| errors | Plate-1-BOP-1000-ACNB-END-3_S10_L004_R2_001.good.fq | 419,096 | 59,315,327 | 100.0% | 141.5 bases | 149 bases | 97.8% |
| total | 2,894,298 | 410,425,472 | 100.0% | 141.8 bases | 149 bases | 98.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 86.5 | 4.5 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 14935 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1439 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.093 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.76459 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 09:24:44 11 Sep 2020 | 09:25:28 11 Sep 2020 | 44 seconds |
| Read alignment to reference genome | 09:25:29 11 Sep 2020 | 09:30:59 11 Sep 2020 | 5 minutes 30 seconds |
| Preprocessing alignments for candidate junction identification | 09:30:59 11 Sep 2020 | 09:31:42 11 Sep 2020 | 43 seconds |
| Preliminary analysis of coverage distribution | 09:31:42 11 Sep 2020 | 09:33:24 11 Sep 2020 | 1 minute 42 seconds |
| Identifying junction candidates | 09:33:24 11 Sep 2020 | 09:33:41 11 Sep 2020 | 17 seconds |
| Re-alignment to junction candidates | 09:33:41 11 Sep 2020 | 09:35:53 11 Sep 2020 | 2 minutes 12 seconds |
| Resolving best read alignments | 09:35:53 11 Sep 2020 | 09:37:04 11 Sep 2020 | 1 minute 11 seconds |
| Creating BAM files | 09:37:04 11 Sep 2020 | 09:38:38 11 Sep 2020 | 1 minute 34 seconds |
| Tabulating error counts | 09:38:38 11 Sep 2020 | 09:39:09 11 Sep 2020 | 31 seconds |
| Re-calibrating base error rates | 09:39:09 11 Sep 2020 | 09:39:12 11 Sep 2020 | 3 seconds |
| Examining read alignment evidence | 09:39:12 11 Sep 2020 | 09:45:44 11 Sep 2020 | 6 minutes 32 seconds |
| Polymorphism statistics | 09:45:44 11 Sep 2020 | 09:45:45 11 Sep 2020 | 1 second |
| Output | 09:45:45 11 Sep 2020 | 09:46:05 11 Sep 2020 | 20 seconds |
| Total | 21 minutes 20 seconds | ||
