mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-3-BOP-27-PFKA-END-4_S64_L001_R1_001.good.fq | 442,583 | 62,752,648 | 100.0% | 141.8 bases | 149 bases | 97.9% |
| errors | Plate-3-BOP-27-PFKA-END-4_S64_L001_R2_001.good.fq | 442,583 | 62,560,812 | 100.0% | 141.4 bases | 149 bases | 98.0% |
| errors | Plate-3-BOP-27-PFKA-END-4_S64_L002_R1_001.good.fq | 408,796 | 58,004,113 | 100.0% | 141.9 bases | 149 bases | 97.9% |
| errors | Plate-3-BOP-27-PFKA-END-4_S64_L002_R2_001.good.fq | 408,796 | 57,843,846 | 100.0% | 141.5 bases | 149 bases | 97.8% |
| errors | Plate-3-BOP-27-PFKA-END-4_S64_L003_R1_001.good.fq | 431,067 | 61,118,767 | 100.0% | 141.8 bases | 149 bases | 97.7% |
| errors | Plate-3-BOP-27-PFKA-END-4_S64_L003_R2_001.good.fq | 431,067 | 60,934,919 | 100.0% | 141.4 bases | 149 bases | 97.9% |
| errors | Plate-3-BOP-27-PFKA-END-4_S64_L004_R1_001.good.fq | 440,593 | 62,452,958 | 100.0% | 141.7 bases | 149 bases | 97.9% |
| errors | Plate-3-BOP-27-PFKA-END-4_S64_L004_R2_001.good.fq | 440,593 | 62,284,100 | 100.0% | 141.4 bases | 149 bases | 97.9% |
| total | 3,446,078 | 487,952,163 | 100.0% | 141.6 bases | 149 bases | 97.9% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 101.6 | 4.1 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 25155 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 61 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.004 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.72892 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 20:26:52 11 Sep 2020 | 20:27:44 11 Sep 2020 | 52 seconds |
| Read alignment to reference genome | 20:27:45 11 Sep 2020 | 20:34:13 11 Sep 2020 | 6 minutes 28 seconds |
| Preprocessing alignments for candidate junction identification | 20:34:13 11 Sep 2020 | 20:35:05 11 Sep 2020 | 52 seconds |
| Preliminary analysis of coverage distribution | 20:35:05 11 Sep 2020 | 20:37:03 11 Sep 2020 | 1 minute 58 seconds |
| Identifying junction candidates | 20:37:03 11 Sep 2020 | 20:37:37 11 Sep 2020 | 34 seconds |
| Re-alignment to junction candidates | 20:37:37 11 Sep 2020 | 20:39:33 11 Sep 2020 | 1 minute 56 seconds |
| Resolving best read alignments | 20:39:33 11 Sep 2020 | 20:40:56 11 Sep 2020 | 1 minute 23 seconds |
| Creating BAM files | 20:40:56 11 Sep 2020 | 20:42:44 11 Sep 2020 | 1 minute 48 seconds |
| Tabulating error counts | 20:42:44 11 Sep 2020 | 20:43:22 11 Sep 2020 | 38 seconds |
| Re-calibrating base error rates | 20:43:22 11 Sep 2020 | 20:43:24 11 Sep 2020 | 2 seconds |
| Examining read alignment evidence | 20:43:24 11 Sep 2020 | 21:13:19 11 Sep 2020 | 29 minutes 55 seconds |
| Polymorphism statistics | 21:13:19 11 Sep 2020 | 21:13:20 11 Sep 2020 | 1 second |
| Output | 21:13:20 11 Sep 2020 | 21:13:48 11 Sep 2020 | 28 seconds |
| Total | 46 minutes 55 seconds | ||
