Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	757,282	Δ69 bp	10.9%	coding (1376‑1444/1767 nt)	sdhA →	succinate dehydrogenase, flavoprotein subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 757281	60 (0.590)	6 (0.060)	6/244	NT	10.9%	coding (1375/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit
	?	NC_000913	757351 =	41 (0.430)					coding (1445/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit

GTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/757131‑757281 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggcgaAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTC  >  NC_000913/757351‑757492                                                                                                                                                                                                                                                                                                       GTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTG                                                                                                                                                    <  5:430071/147‑1      CTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGCGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGA                                                                                                                                                >  1:397108/1‑146                                                              TGCGCGATGGCAGCTAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGT                                                                                              <  5:142896/140‑1                                                              TGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGAA                                                                                                                                               <  7:263544/91‑1                                                                     TGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCC                                                                               <  7:184531/148‑1                                                                         atCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCG                                                                           <  1:276541/146‑1                                                                                CTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGCTCCGGTGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAc                                                                    >  5:297206/1‑147                                                                                                    GATCGCCTGAACCGCTGTAACAATCATCGTAACGGTGAAGATCCGGTGGCGAAAGGGCGTGAGCATTTGAAAGTGATCCGCGAGCGGCTGAAAAATGCCCGTCTGGATGAGACTTCCAGCGAGGTCAACACCCAGCGCGTTGAGTGCC                                                <  3:287683/148‑1                                                                                                                      AACAATAATCGTAACGGTGAAGATCCGGTGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGA                              <  2:397108/148‑1                                                                                                                                                  tgtaGAAAGCGCATGAGAAGTTGAAAGTGATCAGCGAGCGTCTTAAAAATGCCCGTCTTGATCACACTTCCAGCGAGTTAAACACCCAGCGCGTTGACTGCCTGGAACTGGATAACCTGATGGAAACGGCGAATCCAACGGCTGTTTC  >  8:166123/5‑148                                                                                                                                                                                                                                                                                                       GTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/757131‑757281 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggcgaAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTC  >  NC_000913/757351‑757492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.