Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	757,282	Δ69 bp	10.9%	coding (1376‑1444/1767 nt)	sdhA →	succinate dehydrogenase, flavoprotein subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 757281	60 (0.590)	6 (0.060)	6/244	NT	10.9%	coding (1375/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit
	?	NC_000913	757351 =	41 (0.430)					coding (1445/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit

GCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTC  >  NC_000913/757204‑757492                                                                                                                                                    |                                                                                                                                              gCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGaa                                                                                                                                               <  4:434186/148‑1 (MQ=255)       cTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCAGGTGGCGATCCGTAAAGAGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCt                                                                                                                                         >  3:224228/1‑148 (MQ=255)          aTGTTGAAGCGTTTCTGGATCGCCTTAAACGCTGGAACAATAATCGTAAGGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTg                                                                                                                                       <  4:224228/147‑1 (MQ=255)          aTGTTGAAGCGTCTCTGGATCGCCTGAAACGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCt                                                                                                                                         <  1:330481/145‑1 (MQ=255)                      ctctGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGt                                                                                                                          >  3:357216/1‑148 (MQ=255)                      ctctGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCAGGTGGCTATCCGTAACGCGCTGCAAGAATGTATGCAGCATAACTTCTCTGTCTTCCGTGAAGGTGATGCTATGGAGAAATTGCTTGAGCAGTTGAAAGt                                                                                                                          >  7:184445/1‑148 (MQ=255)                              cGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCACGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCcgcg                                                                                                                  <  7:140516/148‑1 (MQ=255)                               gCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCcgcg                                                                                                                  <  4:357216/147‑1 (MQ=255)                                    aaCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCGTTAGCAGTTGAAAGTGATCCGCGAGCGt                                                                                                             <  6:91607/147‑1 (MQ=255)                                               cAATAATCGTAACGGTGAAGATCCGGTTGCGTTCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGGCTTCCTTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGcc                                                                                                 <  6:311456/148‑1 (MQ=255)                                               cAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGcc                                                                                                 <  1:326856/148‑1 (MQ=255)                                                    atCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTc                                                                                             <  6:293963/147‑1 (MQ=255)                                                       gTAACGGGGAAGATCCGGTGGCGATCCGTAAAGAGCTGCAAGAATGTCTGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGAGATCCGCGAGCGTCTGAAAAATGCCCGGCTGGa                                                                                         <  2:325406/148‑1 (MQ=255)                                                        tAACGGTGAAGATCCGGTGGCGATACGTAAAGAGCTGAAAGAATGTATGAAGAATAACTTCTAGGTCTTCCGTGAAGGTGATGAGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGa                                                                                         <  8:184445/147‑1 (MQ=255)                                                         aaCGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGaaaa                                                                                                      <  2:396355/133‑1 (MQ=255)                                                         aaCGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGaaaa                                                                                                      >  1:396355/1‑133 (MQ=255)                                                          tgcgcgatggcagctagtctgatgttgaagcgtctctggatcgcctgaaccgctggaacaataatcgtaacggtgaagatccggtggcgaAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGt                                                                                              <  5:142896‑M2/50‑1 (MQ=255)                                                          tgcgcgatgccagcgagtctgatgttgaagcgtctctggatcgcctgaaccgctggaacaataatcgtaacggtgaagatccggtggcgaa                                                                                                                                               <  7:263544‑M2/1‑1 (MQ=255)                                                                 tgccagcgagtctgatgttgaagcgtctctggatcgcctgaaccgctggaacaataatcgtaacggtgaagatccggtggcgaAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTcc                                                                               <  7:184531‑M2/65‑1 (MQ=255)                                                                 aGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTc                                                                                >  7:287837/1‑147 (MQ=255)                                                                     atcgagtctgatgttgaagcgtctctggatcgcctgaaccgctggaacaataatcgtaacggtgaagatccggtggcgaAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCg                                                                           <  1:276541‑M2/69‑1 (MQ=255)                                                                        gTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCTATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGt                                                                        <  7:374265/148‑1 (MQ=255)                                                                            ctgatgttgaagcgtctctggatcgcctgaaccgctggaacaataatcgtaacggtgaagctccggtggcgaAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTcac                                                                    >  5:297206‑M2/73‑147 (MQ=255)                                                                              aTCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAg                                                                             <  7:73527/137‑1 (MQ=255)                                                                              aTCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAg                                                                             >  8:73527/1‑137 (MQ=255)                                                                                   tAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAg                                                             >  6:402951/1‑148 (MQ=255)                                                                                                gatcgcctgaaccgctgtaacaatcatcgtaacggtgaagatccggtggcgaAAGGGCGTGAGCATTTGAAAGTGATCCGCGAGCGGCTGAAAAATGCCCGTCTGGATGAGACTTCCAGCGAGGTCAACACCCAGCGCGTTGAGTGcc                                                <  3:287683‑M2/96‑1 (MQ=255)                                                                                                gAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGcc                                                >  2:59556/1‑148 (MQ=255)                                                                                                 aaTGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAATGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCt                                               <  7:409706/148‑1 (MQ=255)                                                                                                  aTGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTg                                                      >  4:306033/1‑140 (MQ=255)                                                                                                  aTGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTg                                                      <  3:306033/140‑1 (MQ=255)                                                                                                       tGCAGCATAACTTCTCGGTCTACCGTGAAGGTGATGCGATGGAGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGCGCGGCTGAAAAATGCCCGTCTGGATGACACTGCCCGCGAGTTCAACCCCCAGCGCGTTGAGTGCCTGGAACt                                         >  6:426485/1‑148 (MQ=255)                                                                                                        gcagcaTAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTg                                        >  8:382843/1‑148 (MQ=255)                                                                                                         cagcaTAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTg                                        >  3:343487/1‑147 (MQ=255)                                                                                                               aaCTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCaa                                                                    >  4:304477/1‑113 (MQ=255)                                                                                                               aaCTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCaa                                                                    <  3:304477/113‑1 (MQ=255)                                                                                                               aaCTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAAcc                                 >  5:440915/1‑148 (MQ=255)                                                                                                                  aacaataatcgtaacggtgaagatccggtggcgaAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGa                              <  2:397108‑M2/114‑1 (MQ=255)                                                                                                                   tctcGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGAt                             >  8:91229/1‑148 (MQ=255)                                                                                                                    ctaggTCTTCCGTGAAGTTGATGCGATGTCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGAACGTCTGGATGACACTTCCAGATAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATg                            >  8:291086/4‑148 (MQ=255)                                                                                                                       ggTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGCTCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGaa                         <  3:318726/148‑1 (MQ=255)                                                                                                                       ggTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGaa                         <  1:59556/148‑1 (MQ=255)                                                                                                                        gTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGaaa                        >  5:332000/1‑148 (MQ=255)                                                                                                                            tCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTgg                                                                                          >  7:113641/1‑78 (MQ=255)                                                                                                                            tCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTgg                                                                                          <  8:113641/78‑1 (MQ=255)                                                                                                                             ccGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACgg                     <  7:91229/146‑1 (MQ=255)                                                                                                                              cGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGt                  <  6:440915/148‑1 (MQ=255)                                                                                                                                tGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTAt                >  2:118996/1‑148 (MQ=255)                                                                                                                                     gTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGCGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAAc           >  6:208303/1‑148 (MQ=255)                                                                                                                                              tgtagaAAGCGCATGAGAAGTTGAAAGTGATCAGCGAGCGTCTTAAAAATGCCCGTCTTGATCACACTTCCAGCGAGTTAAACACCCAGCGCGTTGACTGCCTGGAACTGGATAACCTGATGGAAACGGCGAATCCAACGGCTGTTTc  >  8:166123‑M2/7‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                              GCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTC  >  NC_000913/757204‑757492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.