mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-3-BOP-27-ACEF-END-4_S74_L001_R1_001.good.fq | 388,165 | 53,548,444 | 100.0% | 138.0 bases | 149 bases | 95.3% |
| errors | Plate-3-BOP-27-ACEF-END-4_S74_L001_R2_001.good.fq | 388,165 | 53,124,844 | 100.0% | 136.9 bases | 149 bases | 96.9% |
| errors | Plate-3-BOP-27-ACEF-END-4_S74_L002_R1_001.good.fq | 355,388 | 49,012,858 | 100.0% | 137.9 bases | 149 bases | 95.5% |
| errors | Plate-3-BOP-27-ACEF-END-4_S74_L002_R2_001.good.fq | 355,388 | 48,667,715 | 100.0% | 136.9 bases | 149 bases | 96.6% |
| errors | Plate-3-BOP-27-ACEF-END-4_S74_L003_R1_001.good.fq | 377,505 | 52,053,812 | 100.0% | 137.9 bases | 149 bases | 95.2% |
| errors | Plate-3-BOP-27-ACEF-END-4_S74_L003_R2_001.good.fq | 377,505 | 51,640,899 | 100.0% | 136.8 bases | 149 bases | 96.7% |
| errors | Plate-3-BOP-27-ACEF-END-4_S74_L004_R1_001.good.fq | 389,532 | 53,692,942 | 100.0% | 137.8 bases | 149 bases | 95.5% |
| errors | Plate-3-BOP-27-ACEF-END-4_S74_L004_R2_001.good.fq | 389,532 | 53,310,650 | 100.0% | 136.9 bases | 149 bases | 96.6% |
| total | 3,021,180 | 415,052,164 | 100.0% | 137.4 bases | 149 bases | 96.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 75.1 | 3.8 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 49800 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 82 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.005 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.76982 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 00:01:06 12 Sep 2020 | 00:01:51 12 Sep 2020 | 45 seconds |
| Read alignment to reference genome | 00:01:52 12 Sep 2020 | 00:07:37 12 Sep 2020 | 5 minutes 45 seconds |
| Preprocessing alignments for candidate junction identification | 00:07:37 12 Sep 2020 | 00:08:23 12 Sep 2020 | 46 seconds |
| Preliminary analysis of coverage distribution | 00:08:23 12 Sep 2020 | 00:10:02 12 Sep 2020 | 1 minute 39 seconds |
| Identifying junction candidates | 00:10:02 12 Sep 2020 | 00:11:06 12 Sep 2020 | 1 minute 4 seconds |
| Re-alignment to junction candidates | 00:11:06 12 Sep 2020 | 00:12:48 12 Sep 2020 | 1 minute 42 seconds |
| Resolving best read alignments | 00:12:48 12 Sep 2020 | 00:13:59 12 Sep 2020 | 1 minute 11 seconds |
| Creating BAM files | 00:13:59 12 Sep 2020 | 00:15:31 12 Sep 2020 | 1 minute 32 seconds |
| Tabulating error counts | 00:15:31 12 Sep 2020 | 00:16:02 12 Sep 2020 | 31 seconds |
| Re-calibrating base error rates | 00:16:02 12 Sep 2020 | 00:16:05 12 Sep 2020 | 3 seconds |
| Examining read alignment evidence | 00:16:05 12 Sep 2020 | 00:38:58 12 Sep 2020 | 22 minutes 53 seconds |
| Polymorphism statistics | 00:38:58 12 Sep 2020 | 00:38:59 12 Sep 2020 | 1 second |
| Output | 00:38:59 12 Sep 2020 | 00:39:41 12 Sep 2020 | 42 seconds |
| Total | 38 minutes 34 seconds | ||
