breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 692 A→G 6.4% N119S (AAC→AGC)  thrA → Bifunctional aspartokinase/homoserine dehydrogenase 1
RA 24,992 G→A 6.8% A868T (GCT→ACT)  ileS → isoleucyl‑tRNA synthetase
RA 73,807 C→T 6.4% D239N (GAT→AAT)  thiP ← thiamine/thiamine pyrophosphate ABC transporter permease
RA 97,073 Δ1 bp 7.1% coding (1072/1083 nt) mraY → phospho‑N‑acetylmuramoyl‑pentapeptide transferase
RA 98,832 C→T 6.7% L144L (CTG→TTG)  ftsW → putative lipid II flippase; integral membrane protein; FtsZ ring stabilizer
RA 99,900 A→G 5.9% Q86R (CAG→CGG)  murG → N‑acetylglucosaminyl transferase
RA 120,154 Δ1 bp 5.7% intergenic (+19/+24) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 120,158:1 +A 5.7% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 123,061 C→T 100% R15R (CGC→CGT aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
RA 145,596 G→A 5.0% V172V (GTG→GTA yadE → putative polysaccharide deacetylase lipoprotein
RA 167,992 C→A 79.5% S170Y (TCC→TAC)  fhuA → ferrichrome outer membrane transporter
RA 170,457 T→A 6.8% I227N (ATT→AAT)  fhuC → iron(3+)‑hydroxamate import ABC transporter ATPase
RA 177,739 C→T 7.8% D242N (GAC→AAC)  btuF ← vitamin B12 ABC transporter periplasmic binding protein
RA 209,806 T→C 5.2% L43P (CTG→CCG)  ldcC → lysine decarboxylase 2, constitutive
RA 238,653 C→A 100% L28F (TTG→TTT ykfM ← lethality reduction protein, putative inner membrane protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 272,201 A→G 6.7% intergenic (+12/‑379) insN → / → eyeA pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/novel sRNA, function unknown, CP4‑6 propahge
RA 276,134 G→T 9.9% V270V (GTG→GTT mmuP → CP4‑6 prophage; putative S‑methylmethionine transporter
RA 289,256 G→C 7.7% intergenic (‑94/+45) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 329,703 A→T 5.9% K81* (AAG→TAG)  betT → choline transporter of high affinity
RA 370,891 C→A 5.9% P205P (CCC→CCA mhpB → 2,3‑dihydroxyphenylpropionate 1,2‑dioxygenase
RA 410,922:1 +G 6.2% coding (779/909 nt) mak → manno(fructo)kinase
RA 410,927 Δ1 bp 5.7% coding (784/909 nt) mak → manno(fructo)kinase
RA 494,359 T→A 5.6% V95E (GTA→GAA)  ybaB → DNA‑binding protein, putative nucleoid‑associated protein
RA 510,399 C→T 6.1% L327L (CTG→CTA copA ← copper transporter
RA 514,292 C→T 6.1% S100S (TCC→TCT cueR → copper‑responsive regulon transcriptional regulator
RA 535,390 T→C 5.5% V492A (GTG→GCG)  gcl → glyoxylate carboligase
RA 596,677 C→T 5.9% Q360* (CAG→TAG)  cusC → copper/silver efflux system, outer membrane component
RA 600,300 Δ1 bp 6.2% coding (1587/3144 nt) cusA → copper/silver efflux system, membrane component
RA 616,676 C→T 6.9% D840D (GAC→GAT entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 616,888 C→T 6.5% S911L (TCG→TTG)  entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 696,952 T→C 5.2% intergenic (‑13/+11) glnU ← / ← leuW tRNA‑Gln/tRNA‑Leu
RA 725,836 Δ1 bp 5.7% coding (1201/2049 nt) kdpB ← potassium translocating ATPase, subunit B
RA 732,414 G→A 7.1% R944R (AGG→AGA rhsC → Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
RA 745,590 C→T 5.2% S142S (AGC→AGT ybgL → UPF0271 family protein
RA 801,498 T→A 7.6% V247D (GTT→GAT)  ybhH → putative PrpF family isomerase
RA 810,502 A→G 6.1% Y41C (TAT→TGT)  bioF → 8‑amino‑7‑oxononanoate synthase
RA 839,232 T→C 6.0% intergenic (‑25/+17) ybiX ← / ← fiu Fe(II)‑dependent oxygenase superfamily protein/catecholate siderophore receptor
RA 850,996 C→A 6.3% intergenic (+31/+18) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 852,893 C→T 6.0% Q35Q (CAG→CAA mntS ← Mn(2)‑response protein, MntR‑repressed
RA 854,855 T→C 5.3% N297S (AAC→AGC)  ldtB ← L,D‑transpeptidase linking Lpp to murein
RA 860,151 A→G 5.4% intergenic (‑123/+23) ybiV ← / ← ybiW sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase
RA 860,154 A→T 6.4% intergenic (‑126/+20) ybiV ← / ← ybiW sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase
RA 860,157 C→T 5.4% intergenic (‑129/+17) ybiV ← / ← ybiW sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase
RA 861,508 A→T 5.2% S367T (TCT→ACT)  ybiW ← putative pyruvate formate lyase
RA 909,353 G→A 74.0% A566V (GCG→GTG)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
RA 909,422 G→A 18.2% A543V (GCC→GTC)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
JC JC 911,086 IS2 (–) +5 bp 89.1% intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
RA 915,080 G→C 6.2% intergenic (‑223/+272) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 915,081 G→C 6.2% intergenic (‑224/+271) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 937,130 G→T 6.3% E1303* (GAA→TAA)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 943,126 A→G 5.7% E723G (GAA→GGA)  dmsA → dimethyl sulfoxide reductase, anaerobic, subunit A
RA 951,885 T→G 5.6% D557A (GAC→GCC)  pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 964,148 T→C 5.5% intergenic (+36/‑172) ihfB → / → ycaI integration host factor (IHF), DNA‑binding protein, beta subunit/ComEC family inner membrane protein
RA 964,150 T→A 5.5% intergenic (+38/‑170) ihfB → / → ycaI integration host factor (IHF), DNA‑binding protein, beta subunit/ComEC family inner membrane protein
RA 964,152 G→A 5.5% intergenic (+40/‑168) ihfB → / → ycaI integration host factor (IHF), DNA‑binding protein, beta subunit/ComEC family inner membrane protein
RA 965,473 T→A 5.0% L385Q (CTG→CAG)  ycaI → ComEC family inner membrane protein
RA 1,018,983 A→T 8.1% intergenic (+46/+30) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,018,986 A→T 8.1% intergenic (+49/+27) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,035,337 T→A 6.8% L91Q (CTG→CAG)  hyaC → hydrogenase 1, b‑type cytochrome subunit
RA 1,061,193 G→A 5.5% T646T (ACG→ACA torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
RA 1,100,789 G→T 9.8% intergenic (+2/+62) ycdZ → / ← csgG DUF1097 family inner membrane protein/curli production assembly/transport outer membrane lipoprotein
RA 1,100,791 G→C 9.8% intergenic (+4/+60) ycdZ → / ← csgG DUF1097 family inner membrane protein/curli production assembly/transport outer membrane lipoprotein
RA 1,100,793 A→C 9.9% intergenic (+6/+58) ycdZ → / ← csgG DUF1097 family inner membrane protein/curli production assembly/transport outer membrane lipoprotein
RA 1,131,310 A→T 9.8% N98I (AAT→ATT)  flgB → flagellar component of cell‑proximal portion of basal‑body rod
RA 1,134,661 A→G 5.8% intergenic (+104/‑68) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
RA 1,134,668 T→A 5.9% intergenic (+111/‑61) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
RA 1,134,669 T→A 5.9% intergenic (+112/‑60) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
RA 1,186,039 G→A 7.5% V66I (GTC→ATC)  pepT → peptidase T
RA 1,196,220 C→T 8.2% H366H (CAC→CAT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,232 C→T 7.4% T370T (ACC→ACT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,231,314 Δ1 bp 5.5% coding (129/1269 nt) umuC → translesion error‑prone DNA polymerase V subunit; DNA polymerase activity
RA 1,272,535 C→T 5.8% A10V (GCC→GTC)  chaC → cation transport regulator
RA 1,286,917 G→C 7.3% intergenic (+391/+149) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,321,474 A→T 7.8% V491V (GTT→GTA trpE ← component I of anthranilate synthase
RA 1,322,394 C→G 7.1% D185H (GAC→CAC)  trpE ← component I of anthranilate synthase
RA 1,322,396 A→T 8.5% I184N (ATT→AAT)  trpE ← component I of anthranilate synthase
RA 1,322,398 C→G 7.0% V183V (GTG→GTC trpE ← component I of anthranilate synthase
RA 1,337,426 C→T 6.5% G532G (GGC→GGT acnA → aconitate hydratase 1; aconitase A
RA 1,428,527 T→C 6.2% Q67R (CAG→CGG)  insH1 ← IS5 transposase and trans‑activator
RA 1,439,056 T→C 6.1% S577G (AGC→GGC)  pfo ← pyruvate‑flavodoxin oxidoreductase
RA 1,446,548 G→A 6.9% R246C (CGT→TGT)  feaR ← transcriptional activator for tynA and feaB
RA 1,465,487 T→C 5.4% pseudogene (96/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,514,531 T→C 6.0% L234P (CTG→CCG)  ydcU → putative ABC transporter permease
RA 1,534,897 A→T 7.7% intergenic (+28/+68) nhoA → / ← yddE N‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein
RA 1,534,898 A→T 7.7% intergenic (+29/+67) nhoA → / ← yddE N‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein
RA 1,534,904 T→G 7.6% intergenic (+35/+61) nhoA → / ← yddE N‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein
RA 1,534,905 T→C 7.6% intergenic (+36/+60) nhoA → / ← yddE N‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein
RA 1,540,347 C→T 5.9% G748G (GGG→GGA narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,581,360 A→G 5.0% Y389H (TAT→CAT)  ydeN ← putative Ser‑type periplasmic non‑aryl sulfatase
RA 1,654,593 C→T 6.5% W183* (TGG→TGA rspA ← bifunctional D‑altronate/D‑mannonate dehydratase
RA 1,708,844 C→G 18.2% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,725,445 G→A 5.6% A63V (GCG→GTG)  ydhF ← putative oxidoreductase
RA 1,779,124 A→G 6.9% S60P (TCT→CCT)  ydiP ← putative DNA‑binding transcriptional regulator
RA 1,799,167 A→C 5.2% intergenic (‑225/+59) pheS ← / ← pheM phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide
RA 1,799,168 C→A 5.4% intergenic (‑226/+58) pheS ← / ← pheM phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide
RA 1,799,169 T→G 5.4% intergenic (‑227/+57) pheS ← / ← pheM phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide
RA 1,799,170 G→T 5.4% intergenic (‑228/+56) pheS ← / ← pheM phenylalanine tRNA synthetase, alpha subunit/phenylalanyl‑tRNA synthetase operon leader peptide
RA 1,819,955 C→T 5.3% T420T (ACG→ACA chbC ← N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS
RA 1,883,764 A→G 6.5% I193V (ATC→GTC)  yeaV → putative transporter
RA 1,925,158 T→C 6.0% N17N (AAT→AAC holE → DNA polymerase III, theta subunit
RA 1,945,057 C→T 8.3% M100I (ATG→ATA ruvB ← ATP‑dependent DNA helicase, component of RuvABC resolvasome
RA 1,945,113 T→A 8.2% N82Y (AAT→TAT)  ruvB ← ATP‑dependent DNA helicase, component of RuvABC resolvasome
RA 1,945,340 C→T 6.6% R6H (CGT→CAT)  ruvB ← ATP‑dependent DNA helicase, component of RuvABC resolvasome
RA 1,947,884 Δ1 bp 5.9% coding (268/741 nt) yebC ← UPF0082 family protein
RA 1,968,908 T→C 6.0% S153G (AGT→GGT)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,002,222 G→A 5.5% A462V (GCG→GTG)  fliC ← flagellar filament structural protein (flagellin)
RA 2,003,997 T→A 6.1% A42A (GCT→GCA fliD → flagellar filament capping protein
RA 2,008,391 T→C 6.2% W39R (TGG→CGG)  yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein
RA 2,079,004 G→C 8.2% intergenic (+73/+28) yoeF → / ← yeeX pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related/UPF0265 family protein
RA 2,101,165 G→T 5.9% S35Y (TCC→TAC)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating
RA 2,124,684 T→C 5.5% I355V (ATT→GTT)  wcaI ← putative glycosyl transferase
RA 2,124,811 T→C 6.0% Q312Q (CAA→CAG wcaI ← putative glycosyl transferase
RA 2,157,507 C→G 8.0% L749V (CTG→GTG)  mdtB → multidrug efflux system, subunit B
RA 2,167,581 C→T 5.1% intergenic (‑61/+21) ogrK ← / ← yegZ orphan Ogr protein, positive regulator of P2 growth/pseudogene, gpD phage P2‑like protein D
RA 2,171,818 C→T 5.3% intergenic (‑91/+15) gatR ← / ← gatD pseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted/galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,180,168 T→A 6.4% L25H (CTT→CAT)  yegU → ADP‑ribosylglycohydrolase family protein
RA 2,218,252 C→T 5.6% A102A (GCG→GCA yehY ← putative ABC transporter permease
RA 2,240,008 T→C 7.3% N114S (AAC→AGC)  mglB ← methyl‑galactoside transporter subunit
RA 2,253,194 G→A 7.3% L318F (CTT→TTT)  nupX ← nucleoside permease
RA 2,305,264 C→T 6.5% K497K (AAG→AAA mqo ← malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,311,616 C→T 9.6% intergenic (‑82/+30) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
RA 2,311,619 A→G 9.7% intergenic (‑85/+27) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
RA 2,323,783 Δ1 bp 5.3% coding (337/663 nt) atoD → acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
RA 2,348,789 C→A 5.5% intergenic (+21/+33) yfaE → / ← inaA ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase
RA 2,348,790 A→G 5.5% intergenic (+22/+32) yfaE → / ← inaA ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase
RA 2,348,798 T→A 5.3% intergenic (+30/+24) yfaE → / ← inaA ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase
RA 2,348,803 C→T 5.1% intergenic (+35/+19) yfaE → / ← inaA ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase
RA 2,348,804 T→G 5.2% intergenic (+36/+18) yfaE → / ← inaA ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase
RA 2,447,499 C→T 6.0% intergenic (‑26/+9) aroC ← / ← prmB chorismate synthase/N5‑glutamine methyltransferase
RA 2,449,200 G→A 5.8% intergenic (+43/+28) smrB → / ← yfcO putative DNA endonuclease/DUF2544 family putative outer membrane protein
RA 2,449,203 T→C 5.8% intergenic (+46/+25) smrB → / ← yfcO putative DNA endonuclease/DUF2544 family putative outer membrane protein
RA 2,468,115 A→G 6.4% R87R (AGA→AGG gtrA → CPS‑53 (KpLE1) prophage; bactoprenol‑linked glucose translocase/flippase
RA 2,524,438 A→T 5.3% G147G (GGT→GGA xapA ← purine nucleoside phosphorylase 2; nicotinamide 1‑beta‑D‑riboside synthase
RA 2,540,831 T→A 5.7% A283A (GCA→GCT cysW ← sulfate/thiosulfate ABC transporter permease
RA 2,568,153 A→T 6.2% intergenic (‑46/+171) eutH ← / ← eutG ethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase
RA 2,568,154 A→T 6.2% intergenic (‑47/+170) eutH ← / ← eutG ethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase
RA 2,598,014 A→T 6.2% S284S (TCT→TCA bamC ← BamABCDE complex OM biogenesis lipoprotein
RA 2,598,022 G→A 6.5% P282S (CCG→TCG)  bamC ← BamABCDE complex OM biogenesis lipoprotein
RA 2,616,606 G→C 6.6% A171A (GCG→GCC bepA → OM protein maintenance and assembly metalloprotease and chaperone, periplasmic
RA 2,616,609 G→C 6.7% M172I (ATG→ATC bepA → OM protein maintenance and assembly metalloprotease and chaperone, periplasmic
RA 2,639,252 A→T 6.3% V11E (GTA→GAA)  yfgM ← ancillary SecYEG translocon subunit; putative anti‑RcsB factor
RA 2,648,902 G→C 9.3% A1129G (GCA→GGA)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,678,128 T→C 6.8% E78E (GAA→GAG yphE ← putative sugar ABC transporter ATPase
RA 2,712,180 C→T 5.6% A195A (GCG→GCA trmN ← tRNA1(Val) (adenine(37)‑N6)‑methyltransferase
RA 2,720,495 C→T 5.3% G181G (GGC→GGT pka → protein lysine acetyltransferase
RA 2,722,202 T→G 6.1% D750E (GAT→GAG pka → protein lysine acetyltransferase
RA 2,722,492 C→A 75.4% T847K (ACG→AAG)  pka → protein lysine acetyltransferase
RA 2,736,911 C→T 7.6% intergenic (+28/‑243) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,736,913 T→A 7.8% intergenic (+30/‑241) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,736,914 T→A 7.8% intergenic (+31/‑240) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,736,916 A→G 7.7% intergenic (+33/‑238) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,754,208 C→T 5.4% E31K (GAA→AAA)  ratB ← UPF0125 family protein
RA 2,768,609 T→C 5.5% intergenic (+36/‑56) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,768,620 G→A 6.4% intergenic (+47/‑45) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,831,080 C→T 8.5% V404M (GTG→ATG)  norR ← anaerobic nitric oxide reductase DNA‑binding transcriptional activator
RA 2,833,780 C→T 5.2% Y435Y (TAC→TAT norV → anaerobic nitric oxide reductase flavorubredoxin
RA 2,839,621 G→A 6.4% R33H (CGC→CAC)  ascF → cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
RA 2,857,681 C→T 5.2% R197C (CGC→TGC)  mutS → methyl‑directed mismatch repair protein
RA 2,866,487 G→C 8.9% intergenic (+22/+72) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,866,488 G→C 8.6% intergenic (+23/+71) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,873,567 G→A 5.4% R142R (CGC→CGT cysC ← adenosine 5'‑phosphosulfate kinase
RA 2,878,344 C→T 5.0% intergenic (+726/+225) iap → / ← ygbF aminopeptidase in alkaline phosphatase isozyme conversion/CRISPR adaptation ssRNA endonuclease
RA 2,897,538 A→G 6.0% I111I (ATT→ATC ygcS ← putative MFS sugar transporter; membrane protein
RA 2,907,979 A→G 5.2% intergenic (‑38/+50) eno ← / ← pyrG enolase/CTP synthetase
RA 3,004,410 T→A 5.5% L459F (TTA→TTT ygeV ← putative sigma‑54‑interacting transcriptional activator
RA 3,043,920 T→C 5.0% C87R (TGT→CGT)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,045,437 A→G 5.6% D155D (GAT→GAC ygfF ← putative NAD(P)‑dependent oxidoreductase
RA 3,066,299:1 +G 5.0% coding (875/897 nt) ygfI ← putative DNA‑binding transcriptional regulator
RA 3,066,309 T→A 5.4% I289F (ATT→TTT)  ygfI ← putative DNA‑binding transcriptional regulator
RA 3,066,319 Δ1 bp 5.2% coding (855/897 nt) ygfI ← putative DNA‑binding transcriptional regulator
RA 3,076,719 G→A 6.3% V246V (GTC→GTT yggP ← putative Zn‑binding dehydrogenase
RA 3,128,066 G→A 5.0% intergenic (‑45/‑206) glcD ← / → glcC glycolate oxidase subunit, FAD‑linked/glycolate‑inducible glc operon transcriptional repressor; autorepressor
RA 3,155,228 A→G 5.0% intergenic (‑10/‑127) yqhC ← / → yqhD transcriptional activator of yqhD/aldehyde reductase, NADPH‑dependent
RA 3,164,022 C→T 7.7% R651H (CGT→CAT)  parC ← DNA topoisomerase IV, subunit A
RA 3,180,506 A→C 5.9% E29A (GAG→GCG)  ygiC → ATP‑Grasp family ATPase
RA 3,217,580 T→C 5.0% E499E (GAA→GAG aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
RA 3,222,778 A→G 5.3% N49S (AAT→AGT)  ebgA → evolved beta‑D‑galactosidase, alpha subunit
RA 3,232,611 C→T 6.3% A316V (GCT→GTT)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,239,609 T→G 6.4% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,239,611 C→A 6.0% intergenic (+66/‑333) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,241,781 C→T 6.3% intergenic (‑37/+46) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase
RA 3,241,786 C→T 5.5% intergenic (‑42/+41) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase
RA 3,241,790 A→G 5.2% intergenic (‑46/+37) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase
RA 3,241,795 A→G 5.5% intergenic (‑51/+32) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase
RA 3,270,194 G→T 7.8% intergenic (+592/+22) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,195 A→C 8.0% intergenic (+593/+21) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,196 A→G 7.0% intergenic (+594/+20) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,292,913 G→C 6.9% Y141* (TAC→TAG rsmI ← 16S rRNA C1402 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 3,335,746 A→G 6.0% I250T (ATC→ACC)  murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,368,219 G→A 5.9% T131T (ACG→ACA yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,368,220 C→G 5.9% L132V (CTG→GTG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,368,221 T→C 6.0% L132P (CTG→CCG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,382,150 T→C 6.0% intergenic (+40/‑50) degQ → / → degS serine endoprotease, periplasmic/serine endoprotease, periplasmic
RA 3,383,296 T→C 5.5% intergenic (+29/+34) degS → / ← mdh serine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding
RA 3,383,298 T→C 5.4% intergenic (+31/+32) degS → / ← mdh serine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding
RA 3,383,299 G→A 5.4% intergenic (+32/+31) degS → / ← mdh serine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding
RA 3,383,300 T→G 5.4% intergenic (+33/+30) degS → / ← mdh serine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding
RA 3,398,431 A→G 5.1% F149L (TTC→CTC)  mreD ← cell wall structural complex MreBCD transmembrane component MreD
RA 3,473,626 T→C 5.0% A152A (GCA→GCG rpsG ← 30S ribosomal subunit protein S7
RA 3,485,143 G→A 6.6% A218A (GCG→GCA prkB → putative phosphoribulokinase
RA 3,495,641 A→G 5.7% E544G (GAA→GGA)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding
RA 3,514,351 A→G 6.4% D13D (GAT→GAC gph ← phosphoglycolate phosphatase
RA 3,519,557 A→G 5.3% W383R (TGG→CGG)  hofQ ← DNA catabolic putative fimbrial transporter
RA 3,520,486 G→A 5.1% T73I (ACT→ATT) ‡ hofQ ← DNA catabolic putative fimbrial transporter
RA 3,520,487 T→A 5.8% T73S (ACT→TCT) ‡ hofQ ← DNA catabolic putative fimbrial transporter
RA 3,520,488 T→C 5.1% Q72Q (CAA→CAG hofQ ← DNA catabolic putative fimbrial transporter
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,563,154 C→T 6.0% T381M (ACG→ATG)  glpD → sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
RA 3,568,986 G→A 5.3% R115C (CGC→TGC)  glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,575,191 G→A 5.5% V41M (GTG→ATG)  yhgN → UPF0056 family inner membrane protein
RA 3,577,657 A→G 6.0% intergenic (‑65/+74) gntK ← / ← gntR gluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor
RA 3,577,660 C→T 5.9% intergenic (‑68/+71) gntK ← / ← gntR gluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor
RA 3,578,609 T→G 5.7% I40L (ATT→CTT)  gntR ← d‑gluconate inducible gluconate regulon transcriptional repressor
RA 3,578,610 C→A 6.8% K39N (AAG→AAT gntR ← d‑gluconate inducible gluconate regulon transcriptional repressor
RA 3,608,694 T→A 7.9% intergenic (+45/+57) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,646,198 G→A 44.4% A272T (GCC→ACC)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
RA 3,663,450 A→T 25.2% T1013S (ACA→TCA)  mdtF → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,768,131 A→T 100% pseudogene (911/945 nt) rhsJ → Rhs‑family protein
RA 3,781,051 C→G 5.0% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,806,430 C→A 9.0% R213L (CGC→CTC)  waaG ← UDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I
RA 3,964,929 C→T 100% E234E (GAG→GAA rhlB ← ATP‑dependent RNA helicase
RA 4,015,333 A→T 5.4% intergenic (+19/+21) metE → / ← ysgA 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase
RA 4,037,496 A→T 11.5% intergenic (+161/‑23) alaT → / → rrlA tRNA‑Ala/23S ribosomal RNA of rrnA operon
RA 4,041,216 T→C 5.2% Q73R (CAG→CGG) ‡ mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein B
RA 4,041,217 G→A 5.1% Q73* (CAG→TAG) ‡ mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein B
RA 4,041,220 T→C 5.1% S72G (AGC→GGC)  mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein B
RA 4,041,221 G→A 5.1% A71A (GCC→GCT mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein B
RA 4,066,671 C→A 6.7% G175V (GGA→GTA)  yihP ← putative 2,3‑dihydroxypropane‑1‑sulphonate exporter, membrane protein
RA 4,068,014 A→T 5.6% S421R (AGT→AGA yihQ ← putative sulpholipid alpha‑glucosidase; alpha‑glucosyl fluoride glucosidase
RA 4,106,105 C→T 5.2% R96C (CGC→TGC)  cpxP → inhibitor of the cpx response; periplasmic adaptor protein
RA 4,109,196 Δ1 bp 5.9% coding (363/990 nt) sbp → sulfate transporter subunit
RA 4,125,059 A→C 6.0% R584R (CGT→CGG priA ← Primosome factor n' (replication factor Y)
RA 4,145,560 A→G 5.3% V522V (GTA→GTG pflD → putative glycine radical domain‑containing pyruvate formate‑lyase
RA 4,148,661 G→A 10.2% Y535Y (TAC→TAT eptC ← LPS heptose I phosphoethanolamine transferase
RA 4,172,005 T→C 5.3% intergenic (+249/‑52) rrfB → / → murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
RA 4,177,171 T→A 5.7% intergenic (+43/‑187) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
RA 4,198,900 G→A 6.0% G37G (GGG→GGA nfi → endonuclease V; deoxyinosine 3' endonuclease
RA 4,296,060 C→T 31.0% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,448,424 T→A 5.2% intergenic (+189/‑23) ytfP → / → chpS GGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system
RA 4,454,591 G→A 5.3% intergenic (+13/+20) yjfF → / ← fbp putative sugar ABC transporter permease/fructose‑1,6‑bisphosphatase I
RA 4,454,592 T→C 5.4% intergenic (+14/+19) yjfF → / ← fbp putative sugar ABC transporter permease/fructose‑1,6‑bisphosphatase I
RA 4,454,593 G→A 6.2% intergenic (+15/+18) yjfF → / ← fbp putative sugar ABC transporter permease/fructose‑1,6‑bisphosphatase I
RA 4,454,594 T→C 5.4% intergenic (+16/+17) yjfF → / ← fbp putative sugar ABC transporter permease/fructose‑1,6‑bisphosphatase I
RA 4,483,658 A→T 5.8% D60E (GAT→GAA) ‡ valS ← valyl‑tRNA synthetase
RA 4,483,659 T→A 5.1% D60V (GAT→GTT) ‡ valS ← valyl‑tRNA synthetase
RA 4,530,631 T→C 5.0% T341A (ACT→GCT)  sgcX ← putative endoglucanase with Zn‑dependent exopeptidase domain
RA 4,591,807 T→A 5.2% S51T (TCC→ACC)  tsr → methyl‑accepting chemotaxis protein I, serine sensor receptor

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 123064 127549 4486 24 [23] [21] 26 [aceE]–[aceF] [aceE],[aceF]
* * ÷ NC_000913 3423753–3424533 3424533 1–781 25 [20] [23] 25 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3682363 3683198 836 24 [23] [23] 24 dctA C4‑dicarboxylic acid, orotate and citrate transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 39999 =58 (0.740)4 (0.050) 4/242 NT 7.1% coding (388/1143 nt) caiA crotonobetaine reductase subunit II, FAD‑binding
?NC_000913 40052 = 49 (0.650)coding (335/1143 nt) caiA crotonobetaine reductase subunit II, FAD‑binding
* ? NC_000913 167090 =63 (0.800)4 (0.050) 4/238 NT 6.3% coding (2361/2535 nt) mrcB fused glycosyl transferase and transpeptidase
?NC_000913 167109 = 59 (0.790)coding (2380/2535 nt) mrcB fused glycosyl transferase and transpeptidase
* ? NC_000913 274347 =NA (NA)4 (0.050) 3/238 NT NA noncoding (803/1195 nt) IS5 repeat region
?NC_000913 274410 = NA (NA)noncoding (740/1195 nt) IS5 repeat region
* ? NC_000913 274620 =NA (NA)6 (0.080) 4/234 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 381663 =NA (NA)5 (0.070) 5/238 NT NA noncoding (404/1331 nt) IS2 repeat region
?NC_000913 381703 = NA (NA)noncoding (444/1331 nt) IS2 repeat region
* ? NC_000913 = 48418962 (0.790)4 (0.060) 4/230 NT 6.2% coding (215/3150 nt) acrB multidrug efflux system protein
?NC_000913 = 484206 65 (0.900)coding (198/3150 nt) acrB multidrug efflux system protein
* ? NC_000913 629047 =59 (0.750)3 (0.040) 3/238 NT 5.4% coding (497/747 nt) entA 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
?NC_000913 629104 = 50 (0.670)coding (554/747 nt) entA 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
* ? NC_000913 1051877 =81 (1.030)4 (0.050) 4/238 NT 5.1% coding (31/231 nt) ymcE cold shock gene
?NC_000913 1051896 = 73 (0.980)coding (50/231 nt) ymcE cold shock gene
* ? NC_000913 1207790 =17 (0.220)39 (0.570) 34/220 NT 74.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 12 (0.170)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780518 (0.230)29 (0.420) 24/220 NT 67.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 12 (0.170)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1723876 =50 (0.630)3 (0.040) 3/242 NT 5.8% coding (1756/2013 nt) ydhK putative efflux protein (PET) component of YdhJK efflux pump
?NC_000913 1723911 = 49 (0.650)coding (1791/2013 nt) ydhK putative efflux protein (PET) component of YdhJK efflux pump
* ? NC_000913 1735666 =60 (0.760)4 (0.050) 4/242 NT 6.7% coding (289/582 nt) sodB superoxide dismutase, Fe
?NC_000913 1735725 = 53 (0.700)coding (348/582 nt) sodB superoxide dismutase, Fe
* ? NC_000913 2135939 =70 (0.890)4 (0.050) 4/242 NT 5.3% coding (160/444 nt) wzb colanic acid production protein‑tyrosine‑phosphatase; Wzc‑P dephosphorylase
?NC_000913 2135989 = 75 (0.990)coding (110/444 nt) wzb colanic acid production protein‑tyrosine‑phosphatase; Wzc‑P dephosphorylase
* ? NC_000913 2267505 =52 (0.660)4 (0.050) 4/236 NT 7.8% coding (1261/1467 nt) yeiQ putative NAD‑dependent D‑mannonate oxidoreductase
?NC_000913 2267539 = 46 (0.620)coding (1295/1467 nt) yeiQ putative NAD‑dependent D‑mannonate oxidoreductase
* ? NC_000913 2432719 =43 (0.550)4 (0.050) 4/238 NT 9.1% coding (224/1269 nt) folC bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
?NC_000913 2432756 = 39 (0.520)coding (187/1269 nt) folC bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
* ? NC_000913 = 244053460 (0.760)4 (0.050) 4/238 NT 6.3% coding (1072/1221 nt) fabB 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
?NC_000913 = 2440556 62 (0.830)coding (1050/1221 nt) fabB 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
* ? NC_000913 2543915 =62 (0.790)4 (0.050) 4/236 NT 6.2% coding (709/792 nt) ucpA furfural resistance protein, putative short‑chain oxidoreductase
?NC_000913 2543976 = 62 (0.840)coding (648/792 nt) ucpA furfural resistance protein, putative short‑chain oxidoreductase
* ? NC_000913 = 255548850 (0.630)4 (0.050) 4/236 NT 6.7% coding (241/501 nt) eutK putative ethanol utilization carboxysome structural protein
?NC_000913 = 2555515 64 (0.870)coding (214/501 nt) eutK putative ethanol utilization carboxysome structural protein
* ? NC_000913 = 2728364NA (NA)4 (0.050) 4/240 NT NA noncoding (821/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
?NC_000913 = 2728377 NA (NA)noncoding (808/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
* ? NC_000913 = 319474063 (0.800)61 (0.780) 50/250 NT 49.4% noncoding (126/143 nt) sibD sRNA antisense regulator of toxic IbsD protein
?NC_000913 3720633 = NA (NA)noncoding (1/1443 nt) IS150 repeat region
* ? NC_000913 = 362558820 (0.250)64 (0.820) 48/250 NT 76.3% intergenic (+74/+91) yhhI/yhhJ putative transposase/putative ABC transporter permease
?NC_000913 3720630 = NA (NA)pseudogene (3/185 nt) mokA pseudogene, overlapping regulatory peptide, enables hokB; completely contained in another CDS
* ? NC_000913 3676971 =12 (0.150)3 (0.040) 3/238 NT 22.8% coding (1380/2061 nt) yhjG putative inner membrane‑anchored periplasmic AsmA family protein
?NC_000913 3677025 = 9 (0.120)coding (1326/2061 nt) yhjG putative inner membrane‑anchored periplasmic AsmA family protein
* ? NC_000913 4085413 =67 (0.850)3 (0.040) 3/238 NT 5.1% coding (410/3051 nt) fdoG formate dehydrogenase‑O, large subunit
?NC_000913 4085454 = 49 (0.660)coding (369/3051 nt) fdoG formate dehydrogenase‑O, large subunit
* ? NC_000913 = 4271029126 (1.600)7 (0.100) 7/234 NT 5.4% intergenic (+15/+20) yjbR/uvrA DUF419 family protein/ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
?NC_000913 = 4271034 128 (1.750)intergenic (+20/+15) yjbR/uvrA DUF419 family protein/ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
* ? NC_000913 4303065 =126 (1.600)7 (0.090) 6/240 NT 5.8% coding (14/2052 nt) mdtO membrane translocase (MDR) of MdtNOP efflux pump, PET family
?NC_000913 4303092 = 109 (1.450)coding (1018/1032 nt) mdtN membrane fusion protein of efflux pump