New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2827680 =	44 (0.810)	3 (0.060)	3/254	NT	6.8%	coding (319/372 nt)	srlB	glucitol/sorbitol‑specific enzyme IIA component of PTS
	?	NC_000913	2827700 =	39 (0.740)					coding (339/372 nt)	srlB	glucitol/sorbitol‑specific enzyme IIA component of PTS

TTTTCACTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGT  >  NC_000913/2827533‑2827828                                                                                                                                                    |                                                                                                                                                     ttttCACTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACTAAGCGGAATTTCCGGGCACTGACCATGTTGCAGGCCCTGTccc                                                                                                                                                     >  8:92033/1‑149 (MQ=255) ttttCACTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTccc                                                                                                                                                     <  5:98008/149‑1 (MQ=255)  tttCACTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTcccc                                                                                                                                                    >  8:131696/1‑149 (MQ=255)   ttCACTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCCCCCTGCGCTTCGATGGTTTAAACGCAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCcg                                                                                                                                                   >  4:139188/1‑149 (MQ=255)      aCTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGCACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCcgac                                                                                                                                                 >  2:88998/1‑148 (MQ=255)      aCTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACGCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCcgac                                                                                                                                                 <  5:56473/148‑1 (MQ=255)       cTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCcgacga                                                                                                                                               <  2:81095/149‑1 (MQ=255)        tCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCcgacga                                                                                                                                               >  5:17663/1‑148 (MQ=255)                  tCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCgg                                                                                                                                    <  5:5664/149‑1 (MQ=255)                      tATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATc                                                                                                                                >  6:170696/1‑149 (MQ=255)                      gcgacgcgatacccctcggcagccagaccgtggcacaggaacgcgcctaaggtttgcccaccaccgatgacaacggcaacctgattcattttttactccttaacagattcaaacttcaaaaccgatCCCGACGATATCGCGCCGGGATc                                                                                                                                >  7:48998‑M1/127‑149 (MQ=255)                        tCCGGTGACAGCTGTTGGCAGCGTGGCGGAATACTACCTTCGCTAACTGGGTAATGTCACCCTGCGTTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCTTGTCCCCGACGATATCGCGCCGGGATc                                                                                                                                <  2:61649/147‑1 (MQ=255)                             tGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTg                                                                                                                         <  6:239166/149‑1 (MQ=255)                                 cGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGt                                                                                                                     >  2:63677/1‑149 (MQ=255)                                        ggCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATc                                                                                                                                           <  4:181512/120‑1 (MQ=255)                                        ggCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATc                                                                                                                                           >  3:181512/1‑120 (MQ=255)                                          cAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTg                                                                                                            >  6:218538/1‑149 (MQ=255)                                               tGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGAGCGGTTTTGAAGTTTGAATCTGTTAAg                                                                                                       >  2:247015/1‑149 (MQ=255)                                               tGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGGCCTTGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAg                                                                                                       <  7:92033/149‑1 (MQ=255)                                                 gCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGa                                                                                                     <  8:224301/149‑1 (MQ=255)                                                   ggAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAg                                                                                                    >  3:182701/1‑148 (MQ=255)                                                          aaCCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAAt                                                                                            <  4:228618/149‑1 (MQ=255)                                                                cgcgAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAAt                                                                                        <  7:131696/147‑1 (MQ=255)                                                                cgcgAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATc                                                                                       <  3:93062/148‑1 (MQ=255)                                                                 gcgAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCa                                                                                      <  4:7824/148‑1 (MQ=255)                                                                                  aCCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGa                                                                                                                 >  5:20589/1‑104 (MQ=255)                                                                                  aCCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGa                                                                                                                 <  6:20589/104‑1 (MQ=255)                                                                                  aCCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCgg                                                                    <  1:63677/149‑1 (MQ=255)                                                                                   cccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCggt                                                                   >  1:1162/1‑149 (MQ=255)                                                                                    ccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCggtg                                                                  <  7:72878/149‑1 (MQ=255)                                                                                    ccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCggtg                                                                  >  6:52975/1‑149 (MQ=255)                                                                                    ccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCggtg                                                                  >  8:71698/1‑149 (MQ=255)                                                                                       gcgcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGgtggtg                                                               <  4:166909/149‑1 (MQ=255)                                                                                        cgcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCCCTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCggtggt                                                                <  5:218538/147‑1 (MQ=255)                                                                                         gcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGtt                                                                                                                            >  1:232410/1‑86 (MQ=255)                                                                                         gcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGtt                                                                                                                            <  2:232410/86‑1 (MQ=255)                                                                                              gATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCaacc                                                        >  7:252311/1‑147 (MQ=255)                                                                                                  gTTTAAACGAAGGGGAATTTCCGTGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATGGGGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTa                                                    <  6:153004/149‑1 (MQ=255)                                                                                                  gTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTGTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAACCCt                                                      >  5:122971/1‑147 (MQ=255)                                                                                                   tttAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAg                                                   <  3:65419/149‑1 (MQ=255)                                                                                                       aaCGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGgcgc                                               >  5:41766/1‑149 (MQ=255)                                                                                                           aaGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTc                                           <  4:221486/149‑1 (MQ=255)                                                                                                               ggAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCtgt                                       >  1:153141/1‑149 (MQ=255)                                                                                                               ggAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCtgt                                       >  1:169871/1‑149 (MQ=255)                                                                                                                       cGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAAGCTGTTAAGGAGTAAAAAATGAATCAGGGTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGt                               <  1:247015/149‑1 (MQ=255)                                                                                                                            aCTGTCCATGTGCCAGGCCCTGTCCCCGACGATATCGCGCCTGGATCGGTTTTGAAGTTTGAATCTGATAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAAACTTAGGCGCGTTCCTGTGCCACGCTCTGGc                          >  1:21292/1‑149 (MQ=255)                                                                                                                                  cATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGcc                      >  3:220962/1‑147 (MQ=255)                                                                                                                                      tgGCAGGCCCGGTCCCCGACGATCTCGCGCCGGGATCGGTTTTGAAGTTGGAATCTGTTAAGGAGTAAAAAATGAATCAGGTGGCCGGTGGCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAggg                 <  1:88998/148‑1 (MQ=255)                                                                                                                                      acttcaaaaccgatCCCGGCGCGATATCGTCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCtgtg                                      >  7:148242‑M1/15‑127 (MQ=255)                                                                                                                                      acttcaaaaccgatCCCGGCGCGATATCGTCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCtgtg                                      <  8:148242‑M1/113‑1 (MQ=255)                                                                                                                                        gCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTa              >  6:208927/1‑149 (MQ=255)                                                                                                                                                  tCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAACTGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTAtcgcgtcgcg    >  5:190064/1‑149 (MQ=255)                                                                                                                                                  tCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTAtcgcgtcgc     <  5:170696/148‑1 (MQ=255)                                                                                                                                                    CCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGt  <  5:215259‑M1/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TTTTCACTCGGGCAGCATCGCTATCCGGTGACCGCTGTTGGCAGCGTGGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGT  >  NC_000913/2827533‑2827828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.