New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 169764489 (1.100)3 (0.040) 3/242 NT 3.6% coding (397/768 nt) hdhA 7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent
?NC_000913 = 1697668 78 (1.040)coding (373/768 nt) hdhA 7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent
Rejected: Frequency below/above cutoff threshold.

CCCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTTTCCA‑‑T‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1697491‑1697644
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttttttcca‑‑tCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATGCATCATCTAAAGCTGCGGCCAGTCATCTGGTCAGAAATATG  <  NC_000913/1697668‑1697522
                                                                                                                                                                                                                                                                                                               
CCCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTT                                                                                                                                                            >  7:92575/1‑149
 CCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTTT                                                                                                                                                           >  1:36331/1‑149
 CCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTTT                                                                                                                                                           >  3:165285/1‑149
 CCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTTT                                                                                                                                                           <  1:217782/149‑1
   GAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTTTC                                                                                                                                                          >  1:57704/1‑148
    AATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTTTCCA                                                                                                                                                        >  7:72566/1‑149
                                           CAGCTGACTGGCAGCAGCTTTAGAGGATGCATAGGAAGGCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTTTCCA‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGG                                                                                                               <  5:202603/149‑1
                                                                                                                                     GCCACCGCCATTTTTTTCCATTTCTGGCGCAACAAGTTGTGACAG‑‑ATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATGCATC                                       >  1:57131/1‑131
                                                                                                                                     GCCACCGCCATTTTTTTCCATTTCTGGCGCAACAAGTTGTGACAG‑‑ATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATGCATC                                       <  2:57131/131‑1
                                                                                                                                                TTTTTTCCA‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCCGTGGCGTTATTCTGACCATCACTTCTATTGCGGCAGAAAATAAAAATATAAACATCACTTAATATGCATCATCTAAAGCTGCGGCCAGTCATCTGGTCA          >  4:308520/1‑149
                                                                                                                                                 TTTTTCCA‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATGCATCATCTAAAGCTGCGGCCAGTCATCTGGT            >  8:72566/1‑146
                                                                                                                                                  TTTTCCA‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATGCATCATCTAAAGCTGCGGCCAGTCATCTGGTC           <  8:192775/146‑1
                                                                                                                                                      CCA‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATGCATCATCTAAAGCTGCGGCCAGTCATCTGGTCAGAAATA    >  2:243536/1‑149
                                                                                                                                                       CA‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATGCATCATCTAAAGCTGCGGCCAGTCATCTGGTCAGAAATA    <  3:261277/148‑1
                                                                                                                                                        A‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGTCGGTGGCGTTATTCTGACCATCACTTCTATGGAGGCAGAAAATAAAAATATAAACATGAGTTCCTATGAATCATCTAAATATGCGGAAAGTCATATGGTAAGAAATATG  >  5:252749/1‑149
                                                                                                                                                        A‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTAT                                            >  5:315665/1‑107
                                                                                                                                                        A‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTAT                                            <  6:315665/107‑1
                                                                                                                                                        A‑‑TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAAAATGACTACAAATGAATCAAATAAAGCTGCGGCAAGTCATCTGGTCAGAAAAATG  >  2:57704/1‑149
                                                                                                                                                           TCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATTGCGGTTGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATTCATCATCTAAAGCTGAGTCCAGTCATCTGTTCAGAAATATG  >  5:9895/1‑148
                                                                                                                                                                                                                                                                                                               
CCCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGCCACCGCCATTTTTTTCCA‑‑T‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1697491‑1697644
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttttttcca‑‑tCTGTCACAACTTGTTGCGCCAGAAATGGAAAAAAATGGCGGTGGCGTTATTCTGACCATCACTTCTATGGCGGCAGAAAATAAAAATATAAACATGACTTCCTATGCATCATCTAAAGCTGCGGCCAGTCATCTGGTCAGAAATATG  <  NC_000913/1697668‑1697522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.