mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-3-BOP27-SUCB-END-2_S56_L001_R1_001.good.fq | 445,191 | 59,575,407 | 100.0% | 133.8 bases | 148 bases | 98.3% |
| errors | Plate-3-BOP27-SUCB-END-2_S56_L001_R2_001.good.fq | 445,191 | 59,632,106 | 100.0% | 133.9 bases | 148 bases | 97.2% |
| errors | Plate-3-BOP27-SUCB-END-2_S56_L002_R1_001.good.fq | 415,224 | 55,643,819 | 100.0% | 134.0 bases | 148 bases | 98.4% |
| errors | Plate-3-BOP27-SUCB-END-2_S56_L002_R2_001.good.fq | 415,224 | 55,705,403 | 100.0% | 134.2 bases | 148 bases | 96.9% |
| errors | Plate-3-BOP27-SUCB-END-2_S56_L003_R1_001.good.fq | 432,496 | 57,789,330 | 100.0% | 133.6 bases | 148 bases | 98.2% |
| errors | Plate-3-BOP27-SUCB-END-2_S56_L003_R2_001.good.fq | 432,496 | 57,851,520 | 100.0% | 133.8 bases | 148 bases | 96.9% |
| errors | Plate-3-BOP27-SUCB-END-2_S56_L004_R1_001.good.fq | 445,096 | 59,445,034 | 100.0% | 133.6 bases | 148 bases | 98.4% |
| errors | Plate-3-BOP27-SUCB-END-2_S56_L004_R2_001.good.fq | 445,096 | 59,525,073 | 100.0% | 133.7 bases | 148 bases | 97.0% |
| total | 3,476,014 | 465,167,692 | 100.0% | 133.8 bases | 148 bases | 97.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 95.4 | 6.0 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 38286 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 38 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.002 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.73258 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 23:02:04 10 Sep 2020 | 23:02:56 10 Sep 2020 | 52 seconds |
| Read alignment to reference genome | 23:02:57 10 Sep 2020 | 23:09:24 10 Sep 2020 | 6 minutes 27 seconds |
| Preprocessing alignments for candidate junction identification | 23:09:24 10 Sep 2020 | 23:10:16 10 Sep 2020 | 52 seconds |
| Preliminary analysis of coverage distribution | 23:10:16 10 Sep 2020 | 23:12:12 10 Sep 2020 | 1 minute 56 seconds |
| Identifying junction candidates | 23:12:12 10 Sep 2020 | 23:12:46 10 Sep 2020 | 34 seconds |
| Re-alignment to junction candidates | 23:12:46 10 Sep 2020 | 23:14:41 10 Sep 2020 | 1 minute 55 seconds |
| Resolving best read alignments | 23:14:41 10 Sep 2020 | 23:16:04 10 Sep 2020 | 1 minute 23 seconds |
| Creating BAM files | 23:16:04 10 Sep 2020 | 23:17:51 10 Sep 2020 | 1 minute 47 seconds |
| Tabulating error counts | 23:17:51 10 Sep 2020 | 23:18:27 10 Sep 2020 | 36 seconds |
| Re-calibrating base error rates | 23:18:27 10 Sep 2020 | 23:18:29 10 Sep 2020 | 2 seconds |
| Examining read alignment evidence | 23:18:29 10 Sep 2020 | 23:48:53 10 Sep 2020 | 30 minutes 24 seconds |
| Polymorphism statistics | 23:48:53 10 Sep 2020 | 23:48:54 10 Sep 2020 | 1 second |
| Output | 23:48:54 10 Sep 2020 | 23:49:15 10 Sep 2020 | 21 seconds |
| Total | 47 minutes 10 seconds | ||
