New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1604785 =42 (1.210)4 (0.120) 4/256 4.0 8.6% coding (739/993 nt) lsrD autoinducer 2 import system permease protein
?NC_000913 1604815 = 45 (1.370)coding (769/993 nt) lsrD autoinducer 2 import system permease protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGT  >  NC_000913/1604641‑1604921
                                                                                                                                                |                                                                                                                                        
gTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGTGTCGTTTCTGATGCCCGc                                                                                                                                      >  8:7161/1‑149 (MQ=255)
             gcgcGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCgtg                                                                                                                         >  4:4893/1‑149 (MQ=255)
                              cGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCaa                                                                                                        >  5:153145/1‑149 (MQ=255)
                                      aTGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGTGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAt                                                                                                 <  7:7161/148‑1 (MQ=255)
                                                    ccATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggttc                                                                                   <  6:149519/148‑1 (MQ=255)
                                                         aCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGGTTCTGATGCCCGCCATCACCGCCGTGGTGCt                                                                                                                    >  1:90089/1‑110 (MQ=255)
                                                         aCGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGGTTCTGATGCCCGCCATCACCGCCGTGGTGCt                                                                                                                    <  2:90089/110‑1 (MQ=255)
                                                          cGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATc                                                                            <  3:4893/149‑1 (MQ=255)
                                                                tGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                      >  7:134280/1‑149 (MQ=255)
                                                                     tCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATAGTGGTTCCGGTTCCATTATCGGCACCGCCAt                                                                 >  7:110753/1‑149 (MQ=255)
                                                                       tGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                      <  6:158942/142‑1 (MQ=255)
                                                                       tGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                      >  5:158942/1‑142 (MQ=255)
                                                                            tCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATAGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGtt                                                          <  8:110753/149‑1 (MQ=255)
                                                                                        tgctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                                                         <  6:79047/106‑1 (MQ=255)
                                                                                        tgctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                                                         >  5:79047/1‑106 (MQ=255)
                                                                                          ctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTttatta                                                    >  4:61781/1‑141 (MQ=255)
                                                                                          ctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTttatta                                                    <  3:61781/141‑1 (MQ=255)
                                                                                          ctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGata                                            >  1:24377/1‑149 (MQ=255)
                                                                                                  gtgttttGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCAGTTTTATTAGTGGGATATTTGcaa                                     >  3:5684/4‑148 (MQ=255)
                                                                                                     ttttGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAg                                 <  2:24377/149‑1 (MQ=255)
                                                                                                      tttGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAgg                                >  6:60453/1‑149 (MQ=255)
                                                                                                         ggTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGata                                            <  3:10381/134‑1 (MQ=255)
                                                                                                         ggTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGata                                            >  4:10381/1‑134 (MQ=255)
                                                                                                          gTTCAGCACGTTCCGATCTCGGTGCGTCGATTCTGATGACCGCAATCACAGCCGTGGTGCTTGGCGGGGCCAATATTAATGGAGGTTCCGCTTACATTATCGGCACCGCCATTGCGGTTGTATAAGTCGGATATTTGCAACAAGGttag                            >  4:123389/1‑147 (MQ=255)
                                                                                                               gCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCa                                                                                 <  4:133909/91‑1 (MQ=255)
                                                                                                               gCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCAGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAAt                       <  4:5684/149‑1 (MQ=255)
                                                                                                                cACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTg                                                 <  4:26150/122‑1 (MQ=255)
                                                                                                                cACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTg                                                 >  3:26150/1‑122 (MQ=255)
                                                                                                                 aCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATgg                     >  5:69348/1‑149 (MQ=255)
                                                                                                                 aCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATgg                     <  5:60453/149‑1 (MQ=255)
                                                                                                                   gTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCa                   >  8:59202/1‑149 (MQ=255)
                                                                                                                        gATCTCGGTGCGTCGTTTCTGATTCCCGACAAAACCGCCGTGGTGCTTGGCGGGGCAAATATTTATGGTGGTTCCGGTGCCATTATCGGCACCGCCATAGCGGTTTTATAAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAgg              >  8:106213/1‑148 (MQ=255)
                                                                                                                          tctcGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGc                                                                    <  6:100330/93‑1 (MQ=255)
                                                                                                                          tctcGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGc                                                                    >  5:100330/1‑93 (MQ=255)
                                                                                                                              ggTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGc            <  6:153145/145‑1 (MQ=255)
                                                                                                                              ggTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCaaa        >  2:28299/1‑149 (MQ=255)
                                                                                                                                    tcgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAg    <  3:5968/147‑1 (MQ=255)
                                                                                                                                    tcgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAg    >  4:5968/1‑147 (MQ=255)
                                                                                                                                    tcgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGgt  <  8:134280/149‑1 (MQ=255)
                                                                                                                                    tcgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGgt  <  1:28299/149‑1 (MQ=255)
                                                                                                                                              tGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCa     >  2:70089/1‑136 (MQ=255)
                                                                                                                                              tGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCa     <  1:70089/136‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                        
GTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGT  >  NC_000913/1604641‑1604921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.