New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1604785 =42 (1.210)4 (0.120) 4/256 4.0 8.6% coding (739/993 nt) lsrD autoinducer 2 import system permease protein
?NC_000913 1604815 = 45 (1.370)coding (769/993 nt) lsrD autoinducer 2 import system permease protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTC  >  NC_000913/1604671‑1604955
                                                                                                                                                |                                                                                                                                            
cGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCaa                                                                                                                                          >  5:153145/1‑149 (MQ=255)
        aTGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGTGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAt                                                                                                                                   <  7:7161/148‑1 (MQ=255)
                      ccATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggttc                                                                                                                     <  6:149519/148‑1 (MQ=255)
                            cGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATc                                                                                                              <  3:4893/149‑1 (MQ=255)
                                  tGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                                                        >  7:134280/1‑149 (MQ=255)
                                       tCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATAGTGGTTCCGGTTCCATTATCGGCACCGCCAt                                                                                                   >  7:110753/1‑149 (MQ=255)
                                         tGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                                                        >  5:158942/1‑142 (MQ=255)
                                         tGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                                                        <  6:158942/142‑1 (MQ=255)
                                              tCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATAGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGtt                                                                                            <  8:110753/149‑1 (MQ=255)
                                                          tgctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                                                                                           >  5:79047/1‑106 (MQ=255)
                                                          tgctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                                                                                           <  6:79047/106‑1 (MQ=255)
                                                            ctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTttatta                                                                                      <  3:61781/141‑1 (MQ=255)
                                                            ctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTttatta                                                                                      >  4:61781/1‑141 (MQ=255)
                                                            ctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGata                                                                              >  1:24377/1‑149 (MQ=255)
                                                                    gtgttttGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCAGTTTTATTAGTGGGATATTTGcaa                                                                       >  3:5684/4‑148 (MQ=255)
                                                                       ttttGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAg                                                                   <  2:24377/149‑1 (MQ=255)
                                                                        tttGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAgg                                                                  >  6:60453/1‑149 (MQ=255)
                                                                           ggTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGata                                                                              <  3:10381/134‑1 (MQ=255)
                                                                           ggTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGata                                                                              >  4:10381/1‑134 (MQ=255)
                                                                            gTTCAGCACGTTCCGATCTCGGTGCGTCGATTCTGATGACCGCAATCACAGCCGTGGTGCTTGGCGGGGCCAATATTAATGGAGGTTCCGCTTACATTATCGGCACCGCCATTGCGGTTGTATAAGTCGGATATTTGCAACAAGGttag                                                              >  4:123389/1‑147 (MQ=255)
                                                                                 gCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCa                                                                                                                   <  4:133909/91‑1 (MQ=255)
                                                                                 gCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCAGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAAt                                                         <  4:5684/149‑1 (MQ=255)
                                                                                  cACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTg                                                                                   <  4:26150/122‑1 (MQ=255)
                                                                                  cACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTg                                                                                   >  3:26150/1‑122 (MQ=255)
                                                                                   aCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATgg                                                       <  5:60453/149‑1 (MQ=255)
                                                                                   aCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATgg                                                       >  5:69348/1‑149 (MQ=255)
                                                                                     gTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCa                                                     >  8:59202/1‑149 (MQ=255)
                                                                                          gATCTCGGTGCGTCGTTTCTGATTCCCGACAAAACCGCCGTGGTGCTTGGCGGGGCAAATATTTATGGTGGTTCCGGTGCCATTATCGGCACCGCCATAGCGGTTTTATAAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAgg                                                >  8:106213/1‑148 (MQ=255)
                                                                                            tctcGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGc                                                                                                      >  5:100330/1‑93 (MQ=255)
                                                                                            tctcGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGc                                                                                                      <  6:100330/93‑1 (MQ=255)
                                                                                                ggTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGc                                              <  6:153145/145‑1 (MQ=255)
                                                                                                ggTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCaaa                                          >  2:28299/1‑149 (MQ=255)
                                                                                                      tcgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAg                                      <  3:5968/147‑1 (MQ=255)
                                                                                                      tcgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAg                                      >  4:5968/1‑147 (MQ=255)
                                                                                                      tcgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGgt                                    <  8:134280/149‑1 (MQ=255)
                                                                                                      tcgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGgt                                    <  1:28299/149‑1 (MQ=255)
                                                                                                                tGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCa                                       >  2:70089/1‑136 (MQ=255)
                                                                                                                tGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCa                                       <  1:70089/136‑1 (MQ=255)
                                                                                                                    cGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCttt                      >  2:176462/1‑149 (MQ=255)
                                                                                                                        aTCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCgg                  >  1:119559/1‑149 (MQ=255)
                                                                                                                           aCCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTgc               <  1:176462/149‑1 (MQ=255)
                                                                                                                           aCCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTgc               >  2:154261/1‑149 (MQ=255)
                                                                                                                            ccgccgTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGtt                                                                                            >  7:60266/1‑71 (MQ=255)
                                                                                                                            ccgccgTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGtt                                                                                            <  8:60266/71‑1 (MQ=255)
                                                                                                                                        ttGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTAtcgtc  >  6:144890/1‑149 (MQ=255)
                                                                                                                                         tGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTgcgc             <  6:43093/137‑1 (MQ=255)
                                                                                                                                         tGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTagatcggg    >  5:43093/1‑139 (MQ=255)
                                                                                                                                                gCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCgg                  >  7:96753/1‑125 (MQ=255)
                                                                                                                                                gCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCgg                  <  8:96753/125‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
CGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTC  >  NC_000913/1604671‑1604955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.