New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 951207 =108 (1.520)5 (0.070) 5/270 9.1 4.7% intergenic (‑127/+65) pflA/pflB pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase/formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
?NC_000913 951233 = 100 (1.470)intergenic (‑153/+39) pflA/pflB pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase/formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGGCTACGCAGTACGTTTCAACTCGCTGACTAAAGAACAGCAGCAGGACGTTATTACTCGTACCTTCACTCAATCTATGTAATTAGATTTGACTGAAATCGTACAGTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGGCCTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/951354‑951207
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcctttATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTG  >  NC_000913/951233‑951373
                                                                                                                                                                                                                                                                                                 
cgGGCTACGCAGTACGTTTCAACTCGCTGACTAAAGAACAGCAGCAGGACGTTATTACTCGTACCTTCACGCAATCTATGTAATTAGATTTGACTGAAATCGTGCAGTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGG                                                                                                                                                    <  2:1291695/141‑1
CTGGTTACGCAGTACGTTTCAACTCGCTGACTAAAGAACAGCAGCAGGACGTTATTACTCGTACCTTCACTCAATCTATGTAATTAGATTTGACTGAAATCGTACAGTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGG                                                                                                                                                    <  1:663509/143‑1
 TGGCTACGCAGTACGTTTCAACTCGCTGACTAAAGAACAGCAGCAGGACGTTATTACTCGTACCTTCACTCAATCTATGTAATTAGATTTGACTGAAATCGTACAGTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGGC                                                                                                                                                   >  2:378001/1‑143
 TGGCTACGCAGTACGTTTCAACTCGCTGACTAAAGAACAGCAGCAGGACGTTATTACTCGTACCTTCACTCAATCTATGTAATTAGATTTGACTGAAATCGTACAGTAAAAAGCGTACAATAAAGGCTCAACGAAAGTGGGGC                                                                                                                                                   >  2:472531/1‑143
  GGCTACGCAGTACGTTTCAACTCGCTGACTCAACAACATTAGCAGGACGTTATTACTCGTACCTTCACTCAATCTATGTAATTAGATTTGACTGAAATCGTACAGTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGGCC                                                                                                                                                  <  1:42906/143‑1
     TACGCAGTACGTTTTAACTTGTGGACAAAACACTAGCAGCAGGACGTGATTACTCGTACCTTCACTCAATCTATGTAATTAGATTTGACTGAAATCGTACAGTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGGCCTTT                                                                                                                                               <  2:1111699/143‑1
        GCAGTACGTTTAAACTCGCTGACAAAAGAACAGCAGCAGGACGTTATTACTCGTACCTTCACTCAATCTATGTAATTAGATTTGACTGAAATCGTACAGTAAAAAGCGTACAATAAAGGATCCACGAACGTGGAGCCTATATT                                                                                                                                            >  2:341251/1‑143
                                                                                              TGAAATCGTACAGTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGAGAAGGTACGAGTAATAACATACTGCTGCTGTTGTT                                                      >  2:333735/1‑143
                                                                                                          GTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGCCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCGTTAGTCAGCGAGT                                          >  2:529442/1‑143
                                                                                                                           AAGGCTCCACGAAAGTGGGGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACG                                                                      <  1:848841/98‑1
                                                                                                                           AAGGCTCCACGAAAGTGGGGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACG                                                                      >  2:848841/1‑98
                                                                                                                                              GCCTTTTTTGTACGCTTTTTACTGTACGATTTCTGTCAAATCTAATTACATAGAGTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCA      <  1:615711/143‑1
                                                                                                                                                 TTTATTGTACGCTTTTTACTGTACGATTTCAGTCNAATCTANTTACATNGATTGAGTGAAGGTACGANTAATAACGTCCTGCTGCTGTTCTTAAGTCNGCGAGTTGAAACGTACAGCGTAGCCAGATACAAGGATGGTCAGat   >  2:801124/1‑141
                                                                                                                                                  TTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATACATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCCGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCCGCTG  >  1:814684/1‑143
                                                                                                                                                                                                                                                                                                 
CTGGCTACGCAGTACGTTTCAACTCGCTGACTAAAGAACAGCAGCAGGACGTTATTACTCGTACCTTCACTCAATCTATGTAATTAGATTTGACTGAAATCGTACAGTAAAAAGCGTACAATAAAGGCTCCACGAAAGTGGGGCCTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/951354‑951207
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcctttATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTG  >  NC_000913/951233‑951373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.