New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	465407 =	43 (1.070)	3 (0.080)	3/274	5.9	6.6%	coding (141/696 nt)	queC	7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis
	?	NC_000913	465460 =	44 (1.140)					coding (88/696 nt)	queC	7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCAT  >  NC_000913/465265‑465547                                                                                                                                                |                                                                                                                                             aCGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCGGTTcg                                                                                                                                              >  1:322158/1‑143 (MQ=255) aCGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTCAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCCGCGCCAGTTcg                                                                                                                                              >  1:567057/1‑143 (MQ=255)    tATTCGGCATATCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCACGCTACTGACCGCCAG‑CTCGTTGAGCAGCGTTACATCCAGCACCTTATGCGCTCGTGCCCCCAGTTTCAGCGCCAGTTCGCGt                                                                                                                                           <  2:133319/143‑1 (MQ=255)      ttCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTCACCGCCAGACACG‑TGAGCAGGGTGACATCCAGCACCTTATTCTCGCGTGCCCGCAGATTCAGCGCCAGTAcgcgggg                                                                                                                                         >  2:654521/1‑140 (MQ=255)                cATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGAtt                                                                                                                               >  1:476604/1‑143 (MQ=255)                cATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCCTGCCACGTCGCtt                                                                                                                               >  1:278211/1‑143 (MQ=255)                          aGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGAt                                                                                                                     <  2:9042/143‑1 (MQ=255)                          aGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGGCGATTTCTGCGCGAt                                                                                                                     >  1:96925/1‑143 (MQ=255)                           ggTTCATAATCAGGCACTGGAATTCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATg                                                                                                                    <  1:478830/143‑1 (MQ=255)                                aTAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCACCGCCAGTTCCCTTCCCCCGTCGATTTCTGCTCCATGCCCCt                                                                                                               >  1:438081/1‑143 (MQ=255)                                              ggaTGTTGTCACGCGTCGTGCTACTGATCGTCAT‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGTGTGCCCCCAGTTTCAGCGCCAGTTCACGTTCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAAc                                                                                                 <  2:594564/141‑1 (MQ=255)                                                   cTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGGCGGGTGGAAGCCGGCACCTTATGCGCGCGTGCCTCCAGTTTCGGCGCCAGTTCGCGTGCCACGTCGGTTTCTGCGCGATGCCGCCGACCCTAATCGAACgcccc                                                                                            >  2:155811/1‑139 (MQ=255)                                                    tgtgACGCGTCAGGCTGCTGACCGGCAG‑CTGGTTGAGCACGGTCACATCCTGCGCCGTATGCGCGCGTGGCCCCCGTTTCCGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACg                                                                                           <  2:214891/143‑1 (MQ=255)                                                            gTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGAc                                                                                   >  2:38529/1‑143 (MQ=255)                                                               aGGCTACTGGCCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTtc                                                                                <  2:354927/143‑1 (MQ=255)                                                                ggCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCGGCGCGCGTTCGCGTGCCACGTCGATCTCTGCGCGATGCCGCGGACCGTAATCGAACGTCACGCAAGGGACTtca                                                                               >  2:674367/1‑143 (MQ=255)                                                                        aCCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACGTCCTAATAttg                                                                       >  1:128561/1‑143 (MQ=255)                                                                            ccAG‑CTCGGTGAGCAGGGTGACATCCAGCGCCTGATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATtgttgt                                                                   <  1:288729/143‑1 (MQ=255)                                                                                  gcattGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGc                                                              <  1:716554/140‑1 (MQ=255)                                                                                   cggtGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTGCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGcc                                                             <  2:718759/140‑1 (MQ=255)                                                                                   cGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGCCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCCCGCACTGGACTTCATCATATTGTTGTAATGcc                                                             >  2:310141/1‑143 (MQ=255)                                                                                        aGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCAc                                                        <  1:38529/143‑1 (MQ=255)                                                                                               tgtcccccAGCTCCTTATGCGCGCGTGCCCCCAGGTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAg                                                 <  2:473594/137‑1 (MQ=255)                                                                                               tGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTCATGCCTGAACCAGACAg                                                 <  1:446034/143‑1 (MQ=255)                                                                                                gACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCACTGGACTTCATCATATTGCTGTAATGCCTGCACCCGACCgg                                                >  2:671490/1‑143 (MQ=255)                                                                                                 aCATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAggt                                               >  1:434275/1‑143 (MQ=255)                                                                                                    tCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCACGTACGCCTGCCACGTCGCTTTCTGCGCGATGCCGCTGACCGTAATCGCACGTCACGCAATGCACTTCATCATGTTGTCGTAATGCCTGCACCAGACAgggggt                                            >  2:221501/1‑143 (MQ=255)                                                                                                      cAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCCGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATGGCTGTAATGCCTGCACCAGACAGGtggcgg                                          >  2:50175/1‑143 (MQ=255)                                                                                                        gCACCTTATGCGCGCGGGCCCCCCGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACTGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGaa                                        <  2:363132/143‑1 (MQ=255)                                                                                                            cTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGGGGAATccg                                    >  1:1670/1‑142 (MQ=255)                                                                                                              tATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTCCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTCGTGGacccctcg                                  >  2:329766/1‑136 (MQ=255)                                                                                                               gtGCGCCCGGGGCCCCAGGTTCAGCGCCAGGGCGCGTGCCACGTCGGTTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTGCATCATATTGTTGTAATGCCGGCACCAGACAGGTGGTGGAATCCTGAc                                 <  2:382921/142‑1 (MQ=255)                                                                                                                      cgTGCCCCCAGTTTCAGCGCCAGTGCGCGCGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACt                          <  1:88193/143‑1 (MQ=255)                                                                                                                         gCCCTCCGTGTCAGCGCCAGTTCGCTTGCCACGTGAGGTCCTACTCGATGTCGCTGATCGTCATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGTCCTCCACTGaa                       <  2:319023/143‑1 (MQ=255)                                                                                                                         gCCCGCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGaa                       <  2:61041/143‑1 (MQ=255)                                                                                                                         gCCCCCAGTTTCAGAGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGGAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGaa                       <  1:155811/143‑1 (MQ=255)                                                                                                                          cccccAGTTCCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAac                      <  1:715211/143‑1 (MQ=255)                                                                                                                           ccccAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGACCGTCACGCAATGGACTTCATCACATTGTCGTACTGCCTGCACCAGACAGGTCCTCGCATCCTGCCCCCCAccccacc                     >  2:188170/1‑137 (MQ=255)                                                                                                                               aGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAAc                 <  2:476604/143‑1 (MQ=255)                                                                                                                                   tCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATACTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACgcac             >  2:348781/1‑140 (MQ=255)                                                                                                                                     aGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATAttgtt                                                                     <  1:109568/85‑1 (MQ=255)                                                                                                                                     aGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATAttgtt                                                                     >  2:109568/1‑85 (MQ=255)                                                                                                                                              tCGCGTGCCACGGCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCAt  <  1:251455/143‑1 (MQ=255)                                                                                                                                                |                                                                                                                                             ACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAG‑CTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCAT  >  NC_000913/465265‑465547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.