New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	465407 =	43 (1.070)	3 (0.080)	3/274	5.9	6.6%	coding (141/696 nt)	queC	7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis
	?	NC_000913	465460 =	44 (1.140)					coding (88/696 nt)	queC	7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAACC  >  NC_000913/465324‑465599                                                                                                                                         |                                                                                                                                             gTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGAc                                                                                                                                        >  2:38529/1‑143 (MQ=255)    aGGCTACTGGCCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTtc                                                                                                                                     <  2:354927/143‑1 (MQ=255)     ggCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCGGCGCGCGTTCGCGTGCCACGTCGATCTCTGCGCGATGCCGCGGACCGTAATCGAACGTCACGCAAGGGACTtca                                                                                                                                    >  2:674367/1‑143 (MQ=255)             aCCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACGTCCTAATAttg                                                                                                                            >  1:128561/1‑143 (MQ=255)                 ccAGCTCGGTGAGCAGGGTGACATCCAGCGCCTGATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATtgttgt                                                                                                                        <  1:288729/143‑1 (MQ=255)                      gcattGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGc                                                                                                                  <  1:716554/140‑1 (MQ=255)                       cggtGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTGCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGcc                                                                                                                 <  2:718759/140‑1 (MQ=255)                       cGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGCCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCCCGCACTGGACTTCATCATATTGTTGT‑AATGcc                                                                                                                 >  2:310141/1‑143 (MQ=255)                            aGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCAc                                                                                                            <  1:38529/143‑1 (MQ=255)                                   tgtcccccAGCTCCTTATGCGCGCGTGCCCCCAGGTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAg                                                                                                     <  2:473594/137‑1 (MQ=255)                                   tGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑CATGCCTGAACCAGACAg                                                                                                     <  1:446034/143‑1 (MQ=255)                                    gACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCACTGGACTTCATCATATTGCTGT‑AATGCCTGCACCCGACCgg                                                                                                    >  2:671490/1‑143 (MQ=255)                                     aCATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAggt                                                                                                   >  1:434275/1‑143 (MQ=255)                                        tCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCACGTACGCCTGCCACGTCGCTTTCTGCGCGATGCCGCTGACCGTAATCGCACGTCACGCAATGCACTTCATCATGTTGTCGT‑AATGCCTGCACCAGACAgggggt                                                                                                >  2:221501/1‑143 (MQ=255)                                          cAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCCGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATGGCTGT‑AATGCCTGCACCAGACAGGtggcgg                                                                                              >  2:50175/1‑143 (MQ=255)                                            gCACCTTATGCGCGCGGGCCCCCCGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACTGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGaa                                                                                            <  2:363132/143‑1 (MQ=255)                                                cTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGGGGAATccg                                                                                        >  1:1670/1‑142 (MQ=255)                                                  tATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTCCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTCGTGGacccctcg                                                                                      >  2:329766/1‑136 (MQ=255)                                                   gtGCGCCCGGGGCCCCAGGTTCAGCGCCAGGGCGCGTGCCACGTCGGTTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTGCATCATATTGTTGT‑AATGCCGGCACCAGACAGGTGGTGGAATCCTGAc                                                                                     <  2:382921/142‑1 (MQ=255)                                                          cgTGCCCCCAGTTTCAGCGCCAGTGCGCGCGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACt                                                                              <  1:88193/143‑1 (MQ=255)                                                             gCCCTCCGTGTCAGCGCCAGTTCGCTTGCCACGTGAGGTCCTACTCGATGTCGCTGATCGTCATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGTCCTCCACTGaa                                                                           <  2:319023/143‑1 (MQ=255)                                                             gCCCGCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGaa                                                                           <  2:61041/143‑1 (MQ=255)                                                             gCCCCCAGTTTCAGAGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGGAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGaa                                                                           <  1:155811/143‑1 (MQ=255)                                                              cccccAGTTCCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAac                                                                          <  1:715211/143‑1 (MQ=255)                                                               ccccAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGACCGTCACGCAATGGACTTCATCACATTGTCGT‑ACTGCCTGCACCAGACAGGTCCTCGCATCCTGCCCCCCAccccacc                                                                         >  2:188170/1‑137 (MQ=255)                                                                   aGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAAc                                                                     <  2:476604/143‑1 (MQ=255)                                                                       tCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATACTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACgcac                                                                 >  2:348781/1‑140 (MQ=255)                                                                         aGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATAttgtt                                                                                                                          <  1:109568/85‑1 (MQ=255)                                                                         aGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATAttgtt                                                                                                                          >  2:109568/1‑85 (MQ=255)                                                                                  tCGCGTGCCACGGCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCAt                                                      <  1:251455/143‑1 (MQ=255)                                                                                   cacgTGCCACGTCGTTTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACGTCATCATATTGTTGT‑AATGCCTGCACCGGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATa                                                     <  2:278211/141‑1 (MQ=255)                                                                                     cgTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGa                                                   >  2:461403/1‑143 (MQ=255)                                                                                          cACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGACCGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACCCCACGACAGCCCGTTTCATCGATAATc                                              >  2:519472/1‑143 (MQ=255)                                                                                           aCGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATcc                                             >  1:692532/1‑143 (MQ=255)                                                                                             gTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGCGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTg                                           >  1:677818/1‑143 (MQ=255)                                                                                              tCGATTTCTGCGCGATGCCGCTGACCGTAATCGAAGGTCACGCAAGGGACGTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTGTCATAGATAATCCTGc                                          >  1:730248/1‑143 (MQ=255)                                                                                                  tgtCTGCGCGATGCCGCTGACCGTAAGCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGa                                      <  1:248811/141‑1 (MQ=255)                                                                                                      tGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACCGGTGGTGGAATCCTGACCTCCACTGAACACAACGACCGCACGGTTCATAGATAATCCTGCTTgcccca                                  >  1:347057/1‑138 (MQ=255)                                                                                                        ctcgATGCCGCTGACCGTAATCTAACGTCACGCAATGGACTTCTTC‑TTTTCATGTCAACCCCTGCGCCGAACACCTGCTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATa                                <  2:322158/141‑1 (MQ=255)                                                                                                        cgcgATGCCGCTGACCGTAATCGGACGTCACGCAATGGACTTCATCACATTGTCGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATa                                <  1:186132/143‑1 (MQ=255)                                                                                                                            tCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AACGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAAT‑AAAGCGTTATGGTAACGCCTg           <  2:96925/143‑1 (MQ=255)                                                                                                                             cGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTg           >  1:290079/1‑143 (MQ=255)                                                                                                                               aaCGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACAAGACAGGTGGTGGAATCCTGACGTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTCATGGTAACGCCTgac         >  2:487223/1‑141 (MQ=255)                                                                                                                                aCGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGa        <  1:278562/143‑1 (MQ=255)                                                                                                                                      cGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAAcc  <  1:441346/143‑1 (MQ=255)                                                                                                                                         |                                                                                                                                             GTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAACC  >  NC_000913/465324‑465599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.