Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1979486 =0 (0.000)78 (1.200) 52/284 0.2 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 19794890 (0.000)94 (1.450) 54/284 0.1 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255
                                                                                                                                             |                                                                                                                                              
aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATAACAGGGACTTGTTCGCACCTTCCa                                                                                                                                               >  2:303579/1‑143 (MQ=11)
  gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGAGGACTCATGATGCACCCTGTTCTATGGCTCCAGATGACAAACATGATAGCATATCAGGCACTTGTGCGCACCATCCATa                                                                                                                                             >  2:319089/1‑143 (MQ=11)
      aTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGAGACTTGTTCGCACCTTCCATaataa                                                                                                                                         >  1:856336/1‑140 (MQ=1)
          ctTTTCCGCTCTGTCCTCCCTTACTTCTGAATTCACTGTCGGCGAAGGTGAGTTGACGCCCCCTGAGGAACCCTGTTCTATGGCTCCAGATGACCAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                     <  1:908390/143‑1 (MQ=11)
           ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGGTCGCACCTTCCATGACGCTGTAg                                                                                                                                    >  1:1028431/1‑143 (MQ=255)
             ttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACGAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGcc                                                                                                                                  >  1:340067/1‑143 (MQ=255)
              tCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGcca                                                                                                                                 <  1:253807/143‑1 (MQ=40)
                 gggcTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccacca                                                                                                                              <  1:1064537/140‑1 (MQ=255)
                     tGACGGCGCTTACTGCTGAGTGCACTGTCGGCGAAGGTCAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAAc                                                                                                                          <  2:724230/143‑1 (MQ=255)
                       aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAg                                                                                                                        <  1:805859/143‑1 (MQ=255)
                         ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCATCCATAACGCTCTAGCCACCAGAACAGat                                                                                                                      >  2:607760/1‑143 (MQ=255)
                            gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATAtt                                                                                                                   <  2:318252/143‑1 (MQ=255)
                              ttACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGc                                                                                                                 <  1:765863/143‑1 (MQ=255)
                                  tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGCACTTGTTCGCACCTTCCATAACGCTGTAGACCCCCCAACAGATATTgtcgcc                                                                                                             >  1:1111044/1‑138 (MQ=14)
                                    cggAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTATAGCCACCAGAACAGATATTGCGGAACg                                                                                                           <  2:36605/141‑1 (MQ=255)
                                         ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                      <  1:991475/143‑1 (MQ=255)
                                         ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTCGTTCGCACCTTCCATAACGCTGTAGCCACCAGTACAGATATTGCGGAACGACaaa                                                                                                      <  1:223766/143‑1 (MQ=255)
                                            aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAagcg                                                                                                   >  2:110741/1‑141 (MQ=255)
                                             cTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAggaca                                                                                                  >  1:403030/1‑141 (MQ=255)
                                              tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaa                                                                                                 >  1:382286/1‑143 (MQ=255)
                                                tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAAc                                                                                               >  1:846851/1‑143 (MQ=255)
                                                       aGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCa                                                                                        >  1:935573/1‑143 (MQ=255)
                                                             gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACCAAGAGAAACAGAACCAGACTGa                                                                                  >  2:492428/1‑143 (MQ=255)
                                                              ttGATGACTCCTGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGCCAAAGAGAAACAGAACCAGATTGAt                                                                                 <  1:317198/143‑1 (MQ=255)
                                                                  tgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATCATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAt                                                                             <  1:988423/143‑1 (MQ=255)
                                                                   gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                            >  1:354130/1‑143 (MQ=255)
                                                                     cTCATGAGCAACTCTGTTCTATGGCTCCAGTTGACAACCATGATCTCGTATCAGGGACTTGTTCGCACCTTCCAGAACTCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGa                                                                          <  2:465692/143‑1 (MQ=25)
                                                                      tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAAATAATGCGGTACGAGAAAGAGAAACAGAACCAGATTGATGCAGTGAg                                                                         >  2:1129135/1‑143 (MQ=25)
                                                                            tgaCCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCa                                                                   <  1:888148/143‑1 (MQ=255)
                                                                             gacgccTGTTGTCTGGCTCCAGATGACAAGCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGCACAGATATTGCGGATCGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCAt                                                                  <  2:339450/139‑1 (MQ=37)
                                                                                 ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                              >  1:80344/1‑143 (MQ=255)
                                                                                 ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                              >  1:253484/1‑143 (MQ=255)
                                                                                 ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGACTGATGCATTGAGCTTTCATCCTa                                                              >  1:783962/1‑143 (MQ=255)
                                                                                 ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGACTGATGCATTGAGCTTTCATCCTa                                                              >  1:783961/1‑143 (MQ=255)
                                                                                  cTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGCACCCGATTGATGCATTGAGCTTTCATCCTAt                                                             >  1:201903/1‑143 (MQ=255)
                                                                                  cTGTTCTATGCCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTAt                                                             <  2:774394/143‑1 (MQ=255)
                                                                                   tGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGCGCTTTCATCCTATg                                                            >  1:50624/1‑143 (MQ=255)
                                                                                                 aGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAAGAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCCTTGAGCTTTAATTGAATGAAATAAGTTggagt                                              >  2:279400/1‑139 (MQ=37)
                                                                                                 aGATGACAAACATGATCTCATATCAGGGACATGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATACTATGAAATTAATTGCTGt                                              >  2:989862/1‑143 (MQ=255)
                                                                                                  gATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGtt                                             >  2:160481/1‑143 (MQ=255)
                                                                                                      aCAAACATGATCTCATATCAGGGACGTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                         <  1:1032260/143‑1 (MQ=255)
                                                                                                        aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                       >  1:1018815/1‑143 (MQ=255)
                                                                                                        aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGAAATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTGAAAGc                                       >  1:518959/1‑143 (MQ=255)
                                                                                                           cATGGTCGCATAGCCGGGACGTTTGCGCAGCTTGCATAAGGCTGTAGCCACCAGAGCAGATTTTGCGGAACGATAAAGAGTAACAGATCTAGTTTGATGCATTGAGCTTTCATCCTCTGAAATTAATTGCTGTTAAAAGCAtt                                    <  2:176002/143‑1 (MQ=255)
                                                                                                           cATGATCTCATATCAGGGACTTGTTCGCTCCTTGCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                    <  2:269015/143‑1 (MQ=255)
                                                                                                             tgttgtcATATCAGGGACTTCTTCGGACCGTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTgg                                  <  2:366844/138‑1 (MQ=255)
                                                                                                              gATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTggg                                 >  1:716083/1‑143 (MQ=255)
                                                                                                                 ctcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTAc                              >  2:857423/1‑143 (MQ=255)
                                                                                                                         aGGGACTTGTTCGCACCTTCCATACCGCTGTAGCCACCAGACCAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATa                      <  1:534521/143‑1 (MQ=255)
                                                                                                                           ggACTTGTTCGCCCCTGCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTCATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc                    <  2:1023882/143‑1 (MQ=255)
                                                                                                                            gACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTCATTGCTGTTAAAAGCATTGGGTACAGAAAATAccc                   >  1:965655/1‑143 (MQ=255)
                                                                                                                            gACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAccc                   >  2:541917/1‑143 (MQ=255)
                                                                                                                             aCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGGTAAAAGCATTGGGTACAGAAAATACCCa                  >  1:859868/1‑143 (MQ=255)
                                                                                                                                   tCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTc            >  2:311142/1‑143 (MQ=255)
                                                                                                                                      cACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCAt         >  1:873800/1‑143 (MQ=255)
                                                                                                                                       aCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATa        >  2:1021369/1‑143 (MQ=255)
                                                                                                                                         cTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAcc      >  2:806230/1‑143 (MQ=255)
                                                                                                                                         cTTACATAACGCAGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAcc      <  1:607760/143‑1 (MQ=255)
                                                                                                                                          ttCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGATAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAccc     >  1:648290/1‑143 (MQ=255)
                                                                                                                                           tCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCg    <  1:745594/143‑1 (MQ=255)
                                                                                                                                             cATAACGCTGTAGCTAACAGAATAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa  <  1:919912/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.