breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 67,887:1 +G 5.7% coding (451/1503 nt) araA ← L‑arabinose isomerase
RA 67,887:2 +T 5.7% coding (451/1503 nt) araA ← L‑arabinose isomerase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,311,197 G→T 16.2% E37* (GAA→TAA)  tonB → membrane spanning protein in TonB‑ExbB‑ExbD transport complex
RA 1,466,582 T→C 9.3% pseudogene (1191/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,588 C→T 6.0% pseudogene (1197/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,708,930 C→A 9.0% P671Q (CCG→CAG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
JC 2,023,796 Δ5 bp 14.5% intergenic (+118/‑168) fliR → / → rcsA flagellar export pore protein/transcriptional regulator of colanic acid capsular biosynthesis
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,316,394 A→G 39.0% Y73C (TAC→TGC)  rcsB → response regulator in two‑component regulatory system with RcsC and YojN
RA 2,316,681 A→G 53.9% T169A (ACC→GCC)  rcsB → response regulator in two‑component regulatory system with RcsC and YojN
RA 3,527,591 C→T 61.2% Q375* (CAG→TAG)  yrfF → putative RcsCDB‑response attenuator, inner membrane protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,992,438 T→G 38.6% L429* (TTA→TGA)  cyaA → adenylate cyclase
JC JC 3,992,705 IS5 (+) +4 bp 58.3% coding (1553‑1556/2547 nt) cyaA → adenylate cyclase
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,190 A→G 28.9% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 41.6% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 24.5% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 12.5% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2533675 2536437 2763 75 [74] [74] 77 ptsH–[pdxK] ptsH,ptsI,crr,[pdxK]
* * ÷ NC_000913 3423803–3424416 3424416 1–614 75 [74] [74] 75 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 307356 =123 (0.830)6 (0.040) 5/482 NT 5.0% coding (1977/2526 nt) ecpC ECP production outer membrane protein
?NC_000913 307385 = 108 (0.760)coding (1948/2526 nt) ecpC ECP production outer membrane protein
* ? NC_000913 1207790 =80 (0.540)87 (0.630) 65/466 NT 54.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 68 (0.490)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780578 (0.530)73 (0.530) 55/466 NT 50.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 68 (0.490)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 3845531 =109 (0.740)8 (0.060) 8/478 NT 8.3% coding (1568/1767 nt) adeD cryptic adenine deaminase
?NC_000913 = 3992760 73 (0.520)coding (1608/2547 nt) cyaA adenylate cyclase