Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	62 (1.530)	44/284	0.0	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	65 (1.600)	35/284	0.2	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCG  >  NC_000913/2289973‑2290253                                                                                                                                             |                                                                                                                                             aGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGCCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATGTCGGGGCGTTGTCCGCACCTTCCAt                                                                                                                                            >  2:431952/1‑143 (MQ=11)  gcaactctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTCTGGCTCCAGATGACAAACATGCTCCCATATCAGGGACTTGTTCGCACCTTCCaga                                                                                                                                           >  2:127415/6‑141 (MQ=11)      aTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCGCCAGATGACAAACATGATCTTATATCAGGGACTGGTTGGCACCTGCCagagacg                                                                                                                                       >  2:296415/1‑137 (MQ=1)         tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTAACTACACGCGTAGGTTAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                    <  2:320206/143‑1 (MQ=11)          ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                   <  1:588527/143‑1 (MQ=38)              tCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGGCAAACATGATCTCATATCAGGGACCTGTTCGCACCTGCCAGCACGCTGTAGcac                                                                                                                               >  2:212743/1‑141 (MQ=11)               cTGGTCTGACGGTGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCCCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                              <  2:232814/143‑1 (MQ=255)               cTGGTCTGACGGCGCTTACTGCTGAATTCACTGCCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTGGccac                                                                                                                              <  1:668888/143‑1 (MQ=255)                         ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGCTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAgct                                                                                                                    >  1:336677/1‑141 (MQ=255)                         ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGat                                                                                                                    <  1:581491/143‑1 (MQ=255)                              ttACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGc                                                                                                               <  1:488208/143‑1 (MQ=255)                               tACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCg                                                                                                              >  2:448977/1‑143 (MQ=255)                                       aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACa                                                                                                      >  1:423527/1‑143 (MQ=255)                                         ttCACTGTCGGCGAAGGTAGGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                    <  1:353385/143‑1 (MQ=255)                                         ttCACTGGCGGCGAAGGGAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                    <  2:375031/143‑1 (MQ=255)                                              tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaa                                                                                               >  2:56739/1‑143 (MQ=255)                                                    cGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGaa                                                                                         <  1:52560/143‑1 (MQ=255)                                                      aaGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAAcc                                                                                       >  2:63762/1‑143 (MQ=255)                                                           aaGTTGATGCCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGAtt                                                                                  <  2:530699/143‑1 (MQ=255)                                                             gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGa                                                                                <  1:100529/143‑1 (MQ=255)                                                              ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGAt                                                                               >  1:618312/1‑143 (MQ=255)                                                                 atgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCa                                                                            <  2:281553/143‑1 (MQ=255)                                                                  tgaCTCATGATGAACCCTGTTATATGGCTCCAGATGACAAACATGATCTCATGTCAGAGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGACCAGCTATTGCGGACCGACAACGACACACAGCACAAGAGTTAGGCAt                                                                           >  2:653723/1‑143 (MQ=18)                                                                   ttcTCATGATGATCTTTGTTTTATGGCTCTAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                          <  2:346171/141‑1 (MQ=37)                                                                        atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCt                                                                     >  2:253524/1‑143 (MQ=255)                                                                         tgatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATACCGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGCACCGGAGTGATGCATTGAGCtt                                                                    >  2:286068/1‑143 (MQ=255)                                                                          gatgaACCCTGTTCTATGGCTCCAGATGACACACATGATCTCATATCAGGCACTTGTTCGCACCTTCCCTAACGCTGTAGCCACCACAACACACATTCCGGAACGACAACGAGAAACAGAACCAGCCTCATCCATTGCGCttt                                                                   >  2:303644/1‑143 (MQ=18)                                                                          gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGGACGACACAGAGACACACAACCAGATTGATGCATTGAGCttt                                                                   >  1:202467/1‑143 (MQ=255)                                                                          gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGACTGATGCAGCGAGCttt                                                                   >  1:21243/1‑143 (MQ=255)                                                                           atgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTc                                                                  <  1:228069/143‑1 (MQ=255)                                                                                       cTATGGCTCCAGATGACAAACATGATCGCATATAAGGGACTTGTTCGCACCTTCCATAACGCTGTAGACACCAGAACAGATATTCCGGAACGACAAAGAGAAACAGAACTCGAGTAATGCATTCAGAAATCATCCTAGGaaag                                                      >  2:408714/1‑142 (MQ=21)                                                                                             cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTg                                                <  1:56739/143‑1 (MQ=255)                                                                                               acacacaaCAACCACGAACCCACATCAGCGACTTGTCCGCCCCTGCCAGAACGCTGTAGCCACCAGAAAAGACATTGCGGAACGACAAAGAGAAACAGAACCAGATTGACGCACTGAGCTTTCATCCTATGAAATTAATTGCt                                              <  2:384305/136‑1 (MQ=255)                                                                                                cagaacaCAAACCTGATCGCATAGCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTg                                             <  1:267164/137‑1 (MQ=255)                                                                                                 aGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCAACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGt                                            <  2:362188/143‑1 (MQ=255)                                                                                                     gACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaa                                        <  1:714578/143‑1 (MQ=255)                                                                                                      aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACTCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                       <  1:607461/143‑1 (MQ=255)                                                                                                      aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                       <  1:63762/143‑1 (MQ=255)                                                                                                        aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAAGGCTGTAGACACGAGAAGAGATATTGCGGAACGACACAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCATATGAAAGTAATTGCTGTTAAGAGc                                     >  1:506707/1‑143 (MQ=38)                                                                                                           cATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                  >  1:253054/1‑143 (MQ=255)                                                                                                                     tatCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAgca                        >  1:297396/1‑141 (MQ=255)                                                                                                                       tCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGacaa                      >  1:590084/1‑143 (MQ=255)                                                                                                                          ggcaCTTGTTCGTACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGACAAACAGAACCAGATTCATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAc                   <  2:40018/140‑1 (MQ=255)                                                                                                                           ggACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc                  >  2:106010/1‑143 (MQ=255)                                                                                                                               ttGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATa              >  1:367333/1‑143 (MQ=255)                                                                                                                                    cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGATAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTTTGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc         <  1:118561/143‑1 (MQ=255)                                                                                                                                     gCACCTTCCATTACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCa        <  1:448977/143‑1 (MQ=255)                                                                                                                                        ccTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAc     >  2:461983/1‑143 (MQ=255)                                                                                                                                        ccTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTCAAACGCACTGGTTGCGGAAAATACCCATAGCTCCATAc     >  1:195769/1‑143 (MQ=255)                                                                                                                                          ttCCATAACGCTGTAGCCGCCAGAACAGATATTGCGGATCGACAAAGAGTAACCGAACCAGATTGATGCATTGAGCTTTCAGCCTATGAAATTAATTGCTGTTGAATGCATTGGGTACAGAAAATACCCATAGCTCCATAccc   <  2:637263/143‑1 (MQ=255)                                                                                                                                           tCCAGAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGCGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCg  <  2:394729/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                             AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCG  >  NC_000913/2289973‑2290253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.