Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4001645 4001649 5 30 [0] [0] 29 corA magnesium/nickel/cobalt transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40016440 (0.000)29 (0.760) 25/284 0.5 100% coding (219/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913 4001650 = 0 (0.000)coding (225/951 nt) corA magnesium/nickel/cobalt transporter

GATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCG  >  NC_000913/4001513‑4001784
                                                                                                                                         |                                                                                                                                      
tgagtgatcttgtcgaatcggactacgactagcgactgcgtgtacaatctaaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCctt                                                                                                                                   <  1:544168‑M2/6‑1 (MQ=255)
    tgatcgtgccgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttc                                                                                                                               <  1:665687‑M2/10‑1 (MQ=255)
         ttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttctt                                                                                                                          >  1:659076‑M2/129‑143 (MQ=255)
          tgtcggaccggacgacgattagcgactgcgcgtataatttgatcttggtcatagggtggcaacccgccgggaacgggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCttt                                                                                                                         <  1:33613‑M2/16‑1 (MQ=255)
           gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                        <  2:599641‑M2/17‑1 (MQ=255)
              gaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggcactggccgtcatcgatgcatcggcgcgtttctttgaagacgaaaacggcataaatATCCTTCTTCTTAATTGAAg                                                                                                                     >  1:237527‑M2/124‑143 (MQ=255)
                 ctggacgacgacgagtgactgcgcgtacattctgaacttggccagagcctggcaacccgcccggaacgggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATg                                                                                                                  <  1:231721‑M2/23‑1 (MQ=255)
                       gacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAg                                                                                                            >  1:593284‑M2/115‑143 (MQ=255)
                                    tgtgtgtacaatctgaacttggtcggagcctggcaacccgccgggaactggaagacgacgaagcatcggcacggttcggtgaagacgacgacggcctgcatCGCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTaa                                                                                               <  1:82874‑M2/42‑1 (MQ=255)
                                    tgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgactacgtcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTaa                                                                                               >  1:206987‑M2/102‑143 (MQ=255)
                                             aatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgt                                                                                      >  2:36493‑M2/93‑143 (MQ=255)
                                               tctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaaggcgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGCAGCGGCAGAACACGCCGGTAACTCCACTGTgg                                                                                    >  2:434901‑M2/91‑143 (MQ=255)
                                                      ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgcagacgactacggcctgcatCTCCTTCTTCTTCTTTGATGATACGGAAGATCACGCAGGTAACTCAACTAATGCATTTAc                                                                             >  1:629646‑M2/84‑143 (MQ=255)
                                                      ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgacgacgacgacggcctgcctCTCCTTCCTCTTCTTTGCAGATGCGTAAGATCACGCCGGTAACAACAATGTGGAATATAc                                                                             >  1:482104‑M2/84‑143 (MQ=255)
                                                                cctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAt                                                                   <  2:380479‑M2/70‑1 (MQ=255)
                                                                   ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAAATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgt                                                                >  1:571596‑M2/71‑143 (MQ=255)
                                                                         ccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGCGGCATTTACCATCCGTGCtggtcgtccg                                                          >  2:649502‑M2/65‑141 (MQ=255)
                                                                           gccgcggactggaagagagcgaagcagcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                                        <  1:289674‑M2/81‑1 (MQ=255)
                                                                           gcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                                        >  1:143178‑M2/63‑143 (MQ=255)
                                                                           gcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACACCGGTAACTACACTGTGGCATTTACCATCCGTGATGGTCGTCCGtt                                                        >  1:391955‑M2/63‑143 (MQ=255)
                                                                                    tggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCAAGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGATTACTCTgcg                                               >  2:684723‑M2/54‑143 (MQ=255)
                                                                                              cgaagcatgggcaggtttcttctaacacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCATGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGaa                                     <  2:279830‑M2/100‑1 (MQ=255)
                                                                                                aagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAGGATCACGCCGGTAACTCCACTGTGGCATTTAGCATACGTGATGGTCGGATGTTTACTCTGCGTGAGCGTGAACt                                   >  1:612299‑M2/42‑143 (MQ=255)
                                                                                                              ttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTGTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTc                     >  2:686263‑M2/28‑143 (MQ=255)
                                                                                                                 tttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTCTTCGCCTGt                  >  1:691166‑M2/25‑143 (MQ=255)
                                                                                                                       gacgacgacggcctgcatCTTCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCCTTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa            <  2:329843‑M2/125‑1 (MQ=255)
                                                                                                                       gacgacgacggcctgcatCTCCTTCTTCTTCTTTGAATATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa            <  2:10833‑M2/125‑1 (MQ=255)
                                                                                                                                 gcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTCTCGTATGCGTGCCCg  >  1:390569‑M2/9‑143 (MQ=255)
                                                                                                                                 gcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATACGTGATGGGCGTCTGTTTACTCCGCGTGAGCGTGAACTGCCCGCTTTTCTTCGGTACTGTGTGCGtgccag  >  2:34041‑M2/9‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCG  >  NC_000913/4001513‑4001784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.