Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	74 (1.560)	56/284	0.0	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	67 (1.420)	42/284	0.1	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  NC_000913/2101609‑2101887                                                                                                                                             |                                                                                                                                           ttCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTCTTaagga                                                                                                                                          >  2:233475/1‑143 (MQ=255)      aGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATCCTTCCTTTAGGAATTTTCGTTTTATTAAGGAAGGTg                                                                                                                                     >  2:280822/1‑143 (MQ=255)       gTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGc                                                                                                                                    <  1:676299/143‑1 (MQ=255)       gTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGc                                                                                                                                    <  2:777620/143‑1 (MQ=255)        tACATAAAGTAACCTTGATCAAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTCCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCg                                                                                                                                   >  1:59700/1‑143 (MQ=255)         aCATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGa                                                                                                                                  <  2:438934/143‑1 (MQ=255)                gTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCg                                                                                                                           <  2:436502/143‑1 (MQ=255)                gTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGACACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCg                                                                                                                           <  1:441466/143‑1 (MQ=255)                     cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCCCCAATCAACAACCGTATCATAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGCAGGTGCGAATACGCGGGGaa                                                                                                                      >  2:420575/1‑143 (MQ=255)                      ttGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                     <  2:50478/143‑1 (MQ=255)                      ttGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTCAAGGAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                     >  1:807186/1‑143 (MQ=255)                            gAAGCCATTTACACGCACAAAATCTGAAAAACGTACAAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAttcttc                                                                                                               >  1:554240/1‑143 (MQ=255)                                  aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTGTTCTTAAGGAAGCTCCGAATAAGAGGGGAAATTCTTCTCGGCt                                                                                                         >  2:686931/1‑143 (MQ=255)                                        acGCACATAATCTGTAAAACGTAACAGCATAAATTATCCTGCGCACCAATCATCAACCGTATCAGAATAGATACTGTCTTTAGTAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                   <  2:646194/143‑1 (MQ=38)                                            acaAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGGAGAAGCGGGGAAATTCTTCTCGGCTGCCTCCGtca                                                                                               >  1:692526/1‑143 (MQ=255)                                             caAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcat                                                                                              >  1:750118/1‑143 (MQ=255)                                               aaaTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcattt                                                                                            <  2:152102/143‑1 (MQ=255)                                                  tCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                                                         <  1:236383/143‑1 (MQ=255)                                                       aaaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCAGAGCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCTAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                    <  1:307696/143‑1 (MQ=255)                                                         aaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCa                                                                                  >  1:297511/1‑143 (MQ=255)                                                            gTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTCTATTCTCGGCGTTGTGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGt                                                                               >  1:625580/1‑143 (MQ=37)                                                            gTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTCTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCCTCTCATCTCTTCATGt                                                                               >  2:529264/1‑143 (MQ=255)                                                             tACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGtt                                                                              >  1:644008/1‑143 (MQ=255)                                                               ccAGCATAAACGGTCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTg                                                                            <  1:667915/143‑1 (MQ=255)                                                                cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                           <  2:86833/143‑1 (MQ=255)                                                                  gCATAAATGATCCTGCGCACCAAACAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                         <  1:682099/143‑1 (MQ=255)                                                                        aTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGCAGGTGCGAAGAAACGGGGAAACTCTTCTCGGCTGGCTCAGTCATTTCATTTATTCATCTTTTCGCCGtttt                                                                   >  1:317221/1‑143 (MQ=255)                                                                         tGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                                                                  <  1:856196/143‑1 (MQ=255)                                                                          aaTCCCGCTCACCAATCAACAACCGTCTCAGAATAGATACTTTCTTTAGGAATTTTCGTTTTATTAAGTAAGGTTCGAATAAGCGGGGAAATTCTGCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                                                                 <  1:137986/142‑1 (MQ=21)                                                                                     ccAATCAACTACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                      <  2:224732/143‑1 (MQ=255)                                                                                     ccAATCAACCTCCGTACCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                      <  1:530514/143‑1 (MQ=255)                                                                                          caacaaCCGGATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCt                                                 <  2:194755/143‑1 (MQ=255)                                                                                               acCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAt                                            >  1:422823/1‑143 (MQ=255)                                                                                                   tATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                        <  1:747784/143‑1 (MQ=255)                                                                                                        gAATAGATACTTTCTTTAGGAATTTTTGTTTTATGAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAtt                                   >  1:164250/1‑143 (MQ=255)                                                                                                            aGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATTAGCCTATTTAGa                               >  1:570863/1‑143 (MQ=255)                                                                                                            aGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGa                               >  2:519173/1‑143 (MQ=255)                                                                                                               tACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGCGCCGTTTTTTTCTCCCGTAAATGCCTTAAAACAGCATATTTAGACCg                            >  1:697190/1‑143 (MQ=17)                                                                                                                  tttctttcGGAATTTTCGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATATTTGAGCCGGTTTTTTCTCCCGTAACTCCCTTGAATCGTCCTAGTTAgcctgtct                         >  2:249100/1‑136 (MQ=14)                                                                                                                          ggAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCa                 >  1:31300/1‑143 (MQ=255)                                                                                                                            aaTTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGTCTTGAATAAGCTTATTTAGACCGTTTCTTCGCCttt               >  2:658126/1‑143 (MQ=255)                                                                                                                              tttttGTTTTCTTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTTATTTCTTTTCTTCATGTTTTAGCCGATTTTTTTTCTCTTTATTCTCTTGCATCATCCTCTTTATACCTTTTTTTTGCCTTTTa             >  2:672990/1‑143 (MQ=2)                                                                                                                                 ttGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAgg          >  1:769732/1‑143 (MQ=37)                                                                                                                                    ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTTGCCATTTCAGGGGt       >  2:154137/1‑143 (MQ=255)                                                                                                                                    ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTCCGCCATTTAAGGCGt       >  2:696441/1‑143 (MQ=21)                                                                                                                                     tttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGTCTGACTCAGTCATTTCATTTCTTCATGTATGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTTCGCCCTTTAAGCCGtt      >  2:810986/1‑143 (MQ=11)                                                                                                                                         ttaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATGTCATTTCTTCAGGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATct  >  1:635910/1‑142 (MQ=11)                                                                                                                                             |                                                                                                                                           TTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  NC_000913/2101609‑2101887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.