Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,553,103	IS5 (+) +4 bp	coding (19‑22/2706 nt)	malT →	mal regulon transcriptional activator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	49 (0.870)	38/284	0.5	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3553103 =	0 (0.000)					coding (19/2706 nt)	malT	mal regulon transcriptional activator
*	?	NC_000913	= 2290113	NA (NA)	81 (1.450)	49/282	0.1	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3553106	0 (0.000)					coding (22/2706 nt)	malT	mal regulon transcriptional activator

GGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289057‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGAAAGACCACCCTCATTTCCCAGTGGGC  >  NC_000913/3553103‑3553242                                                                                                                                                                                                                                                                                         ggtTAACGGCGTAAATGGGGAAGAAACGGTCTAAATAGGCTGATTTAAGGCATTTACGGGAGAAAATATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAA                                                                                                                                          <  2:67259/140‑1        GCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAAGATGAAGAAATGAAATGACTGAGTCAGCCGCGAAGAATTTCCCCGCTTATTCGCACCTTCCCTACGTCGTCC                                                                                                                                   >  1:853722/1‑143          caacaacGGCTAAGACACGGTCTAAATAGGCTGATTCCAGGCAGTTACGGGAGAAAAAATCGGCTCAAACATGAACAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGG                                                                                                                                 <  2:597498/136‑1          CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTAGCGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCACACCTTCCCTAAGACGTCCGG                                                                                                                                 >  2:309187/1‑143            cAAATGGCGAAGAAACGTTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGCTTTCCCTCGCTTTTTCGCACCTTCCCTAAGTCGTCCGGTT                                                                                                                               <  1:697819/142‑1            TAAATGGCGAAGACACGGTCTCGATAGGCTGATTCAAGTCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTT                                                                                                                               <  2:232895/143‑1            TAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTT                                                                                                                               <  1:885028/143‑1             AAAGGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTC                                                                                                                              <  1:940804/143‑1                  gcaAAGAAACGGGCTACATAGGCTGATTCAAGGCATTTACGGGGGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTC                                                                                                                         <  1:432874/140‑1                  GCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGACATGTACTTATTTAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTC                                                                                                                         <  1:638334/143‑1                    GAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGA                                                                                                                       >  1:543845/1‑143                    GAAGAAACGGTATAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTACCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCCACGCGc                                                                                                                       >  2:134574/1‑142                     AAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGAC                                                                                                                      >  1:190847/1‑143                      AGAAACGGGCTAAAGAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACC                                                                                                                     <  1:408751/143‑1                              gCTAAATAGGCTGATTCAAGGCATGTACGGGAGAAAAAGTCGGCTAAAACATGTAGTAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTG                                                                                                             <  2:352366/142‑1                                  AATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTC                                                                                                         >  2:580539/1‑143                                      GGCTGAGTCAAGGCAGTTACGGGAGAAAGTATCGTCGCGAGCATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATGCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGA                                                                                                     <  2:759674/143‑1                                      GGCAGATCCAAGGCAAGTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGA                                                                                                     <  1:344306/143‑1                                         TGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGCCGTCCGGTTCGACTCCCCCATCGCGTCGTTCTTGccgc                                                                                                  >  2:474212/1‑139                                          ggcgCAAGGCATCTACGGGAGAAACAATCCGCTCAAACATGAAGAAATGAAAGGACTGAGTCAGCCGAGAAGAATTCCCCCGCTTATCCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGC                                                                                                 <  1:164874/139‑1                                          GATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGC                                                                                                 <  1:887982/143‑1                                               AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGGCCGTCACGTCGTCCGTGAGCGCCTGCT                                                                                            >  2:115366/1‑143                                                      TTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTACGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAA                                                                                     <  1:428349/143‑1                                                            GAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACATTCC                                                                               >  1:870554/1‑143                                                              GAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGG                                                                             >  2:571055/1‑143                                                                acatATCGACTCAAACAGGAATAAGTGATATGACTGAGTCAGCCGAGAAGAAGTTCCCCGCTTATTCGCACTTTCCCTATGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCG                                                                           <  2:605681/139‑1                                                                  AAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACCCGCCCATACCGCGGTTCGTGAGCGCCTGCTGGCCAAACCTTCCCGCGCG                                                                         >  2:321048/1‑143                                                                        GCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTATGTCGTGCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCCGGCTGCCCTTTCCGGCTCGAACAAC                                                                   >  1:141838/1‑143                                                                            AAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCC                                                               >  1:80130/1‑143                                                                                 TGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTG                                                          <  1:698532/143‑1                                                                                  GAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGG                                                         <  1:423047/143‑1                                                                                   AAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGCCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTACCGGCGCGAACAACTTGCGGCTGGg                                                        >  1:91130/1‑142                                                                                    AGAAATGAAGTGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCG                                                       <  2:549196/143‑1                                                                                      AAATGAAATGACTGAGTCAGCCGACAAGAATCTCCCCGCGTGTTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCT                                                     <  1:957385/143‑1                                                                                        agGAAATGACTGAGTCAGCCGAGAAGAGTTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGA                                                   <  2:182668/141‑1                                                                                         TGAAATGACTGCGTCAGCCGAGAAGAGTTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGAT                                                  <  1:752805/143‑1                                                                                            AATGACTGAGGCAGCCGAGAAGAAGAGCCCCGCGGATGCGCGCCTGCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCAC                                               <  1:12248/143‑1                                                                                              TGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGA                                             <  1:288684/143‑1                                                                                              TGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTAACCGGGGCTAACAACTTCCGGCTGGCGCTGATCACGA                                             <  2:559072/143‑1                                                                                              TGACTGAGTCAGCCGAGAAGAATTCCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGA                                             <  1:459952/143‑1                                                                                                      TCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGCCCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAAATTTCCGGCGCGAACAACTTCCGGCTGGCGCTGGTCACGAGAGCTGCG                                     >  1:133531/1‑143                                                                                                               AAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGGTGGAGGGGCCACCGCGAACAACTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGA                            >  1:465944/1‑143                                                                                                                      TCCCCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGAAAGACCA                     <  1:571055/143‑1                                                                                                                         CCGCTTATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACACCTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGAAAGACGACCC                  >  1:608787/1‑143                                                                                                                               ATTCGCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGAAAGACCACCCTCATTT            >  2:705479/1‑143                                                                                                                                   GCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGAAAGACCACCCTCATTTCCCA        <  2:543845/143‑1                                                                                                                                   GCACCTTCCCTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGAGGCCTGCGGGCTACAGAGAGACCAAGCTCGTATCCCA        >  1:120157/1‑143                                                                                                                                   GCACCTTCCCGAACTCGCCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGAAAGACCACCCTCATTTCCCA        <  2:708413/143‑1                                                                                                                                         gCCCGAAGTCGGCCGGGGCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGAAAGACCACCCTCATTTCCCAGTGGGC  <  1:954188/142‑1                                                                                                                                                                                                                                                                                         GGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289057‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAAGTCGTCCGGTTCGACTCGACCATACCGTGGTTCGTGAGCGCCTGCTGGCTAAACTTTCCGGCGCGAACAACTTCCGGCTGGCGCTGATCACGAGTCCTGCGGGCTACGGAAAGACCACCCTCATTTCCCAGTGGGC  >  NC_000913/3553103‑3553242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.