Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,024,155	IS5 (+) +4 bp	coding (188‑191/624 nt)	rcsA →	transcriptional regulator of colanic acid capsular biosynthesis

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2024155 =	0 (0.000)	60 (1.160)	49/282	0.1	100%	coding (188/624 nt)	rcsA	transcriptional regulator of colanic acid capsular biosynthesis
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 2024158	0 (0.000)	77 (1.480)	54/282	0.0	100%	coding (191/624 nt)	rcsA	transcriptional regulator of colanic acid capsular biosynthesis
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  NC_000913/2101607‑2101887                                                                                                                                               |                                                                                                                                           ccTTCACAGTAAATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAGATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaag                                                                                                                                            <  2:868208/142‑1 (MQ=255)                aaGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAg                                                                                                                             <  1:239322/143‑1 (MQ=255)                       cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGaa                                                                                                                      >  1:687034/1‑143 (MQ=255)                         tGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                                    >  1:524568/1‑143 (MQ=255)                         tGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATCTCTCTTTTCTTAAGGGAGGCACGCATCAGCGAGGAAAt                                                                                                                    >  2:585457/1‑143 (MQ=255)                             cGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATCGATACATTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTTCGAGTAAGCGTGAATAttctt                                                                                                                >  2:110580/1‑143 (MQ=255)                                   cATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCTGGGAAATTCTTCTCGGc                                                                                                          >  2:510451/1‑143 (MQ=255)                                    aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAACTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCt                                                                                                         <  1:204806/143‑1 (MQ=255)                                    aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGGGCGACTAAGCGGGCAAATTCTTCTCGGCt                                                                                                         >  1:336952/1‑143 (MQ=255)                                       tACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                      <  1:713421/143‑1 (MQ=255)                                       tACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                      <  2:840477/143‑1 (MQ=255)                                       tACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                      >  1:783725/1‑143 (MQ=255)                                         cacGCACAAAATCTGAAAAACGTAACAGGATGGAGGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTc                                                                                                    >  1:606661/1‑143 (MQ=38)                                          acGCACAAAAGCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTGTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                   <  1:855011/143‑1 (MQ=255)                                            gCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTACGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAccgagc                                                                                                 >  2:868287/1‑138 (MQ=255)                                            gCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTCTAGGAAGTATTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACGCAGt                                                                                                 >  2:33282/1‑143 (MQ=255)                                             cacaAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGCTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtc                                                                                                >  1:68504/1‑143 (MQ=255)                                              acaAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtca                                                                                               >  2:442086/1‑143 (MQ=255)                                               caAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACACCCGTCTCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcat                                                                                              <  2:109865/143‑1 (MQ=255)                                                aaaaTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatt                                                                                             >  2:899888/1‑143 (MQ=255)                                                aaaaTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGCTTTATTAAGGAAGGTGCGAATAAGCGGGGAACTTCTTCTCGGCTGACTTAGtcatt                                                                                             >  2:434944/1‑143 (MQ=255)                                                    tCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATCCTTTCTCTCGGCATTTTTGTTTTATTAACGAAGGTGCGAATAAGCGGGGAAATTCTTCTCCGCTCACTCAGtcatttcat                                                                                         >  1:655993/1‑143 (MQ=37)                                                    tCTAAAAAACGTACTAGCATAAATGAACCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                                                         <  2:219103/143‑1 (MQ=255)                                                      tGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTcatttcattt                                                                                       >  1:129705/1‑143 (MQ=255)                                                       gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                      >  1:862217/1‑143 (MQ=255)                                                       gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                      >  2:672048/1‑143 (MQ=255)                                                       gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                      <  1:640868/143‑1 (MQ=255)                                                        aaaaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttct                                                                                     >  1:936105/1‑143 (MQ=255)                                                          aaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGTAAGGTGCGAATAAGCGGTGTATTTCTTCTTGGCTGATTCAGTCATTTCGTttcttt                                                                                   >  2:839062/1‑142 (MQ=255)                                                          aaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttcttc                                                                                   <  2:783725/143‑1 (MQ=255)                                                             cGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATg                                                                                <  1:747412/143‑1 (MQ=255)                                                                  cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                           <  2:115047/143‑1 (MQ=255)                                                                              tCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                               <  2:687034/143‑1 (MQ=255)                                                                                  gctcACCAATCAACATCCGTATCAGAATAGATACTTTCTTTAGGAATGTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCTTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTccc                                                           <  1:494701/140‑1 (MQ=255)                                                                                      aCCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAATCGGGGAACTTCTTCTCGGCTGACTCAGTCATTTGATTTCGTCATGTTTTAGCCTCTTCTTTCTGCCTTac                                                       >  2:594295/1‑142 (MQ=21)                                                                                            caacaaCCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCt                                                 <  2:606661/143‑1 (MQ=255)                                                                                            caacaaCCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATCTTTTCTCCCGTAACTGCCt                                                 >  1:708022/1‑143 (MQ=255)                                                                                             aacaacCGTATCAGAATAGATACTTCCTCTAGGAATATTTGCATTATTACCATAGATACGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTACTTCATGTTTGAGCCGATCCTTTCTCCCGCAAATGCCta                                                >  2:290518/1‑142 (MQ=17)                                                                                                aacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTCTGAATAAGCGGCGAAATTATTCTCGGCTAATTCATTGTTTTCATTTCGTCATGTTTGTGCCGATTTTTTCTCCCTTAAATGTCTTgtc                                             >  2:573139/1‑141 (MQ=255)                                                                                                aacCGTATCAGAATAGAAACTTTATTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAATTTCTTCTCGGCTGACTTAGTCATTTCATTTATTCTTGTTTGAGCTGAGTGTTTATTCCGTACATGCTTTGaa                                             >  2:343795/1‑143 (MQ=18)                                                                                                 gccGTATCAGAATAGATACTTTCTTTAGCAATTTTTGTTTTATTAAGGACGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAt                                            <  2:585099/142‑1 (MQ=255)                                                                                                   cGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTATTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCTTAAATGCCTCGAATCa                                          >  2:588045/1‑143 (MQ=255)                                                                                                     tATCAGAATAGATCCTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                        <  1:609833/143‑1 (MQ=255)                                                                                                        cAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGACTCGGCCTa                                     >  1:554601/1‑143 (MQ=255)                                                                                                         aGAATAGATACTTTCTATAGGAATTTTTGTTTTATTAAGGAAGGTACGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAt                                    >  1:606310/1‑143 (MQ=255)                                                                                                          gAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGCGCCGATTTTTTCTCCCGTAAATGCCTTGCATCTGCCTAtt                                   >  2:546443/1‑143 (MQ=255)                                                                                                                aTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGGGCCGAGTGTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                             <  2:524568/143‑1 (MQ=255)                                                                                                                aTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTCGAGCCGATTTTTTCTCCCGTAGCTGCCTTGAATCAGCCTATGTAGAcc                             >  1:296257/1‑143 (MQ=255)                                                                                                                   ctttctttAGGAATTTTTGTTTTATTACGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGtt                          <  2:516387/143‑1 (MQ=255)                                                                                                                     ttcattAGGAAGTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttc                        <  2:866936/143‑1 (MQ=255)                                                                                                                        tttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc                     <  1:877184/143‑1 (MQ=255)                                                                                                                         ttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                    >  2:506207/1‑143 (MQ=255)                                                                                                                             gAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAt                >  1:824006/1‑143 (MQ=255)                                                                                                                             gAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGACTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAt                >  1:809332/1‑143 (MQ=255)                                                                                                                                  tttGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAg           >  1:535445/1‑143 (MQ=38)                                                                                                                                     gTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTCCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg        <  1:193171/143‑1 (MQ=25)                                                                                                                                      ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                    <  2:540476/66‑1 (MQ=40)                                                                                                                                      ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                    >  1:540476/1‑66 (MQ=40)                                                                                                                                          attaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCAGAGTTGAGCCCATTTTTTCTCCCGTACATGCCCTTAATCCGCCTACTTAGACCGGTCCTTCGCCACTTAAGGCgccagc   >  2:187769/1‑138 (MQ=11)                                                                                                                                           ttaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc  >  1:923803/1‑143 (MQ=11)                                                                                                                                               |                                                                                                                                           TCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  NC_000913/2101607‑2101887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.