Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	52 [0]	[0] 52	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	52 (1.000)	47/282	0.1	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

TTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGC  >  NC_000913/4001511‑4001788                                                                                                                                            |                                                                                                                                           atggagtggtgttgtcgaccgggacgccgacgagcgactgcgcgtacaatctgaacttggccagggcctggcaacccgcccggagctggaaggcatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                         <  1:423330‑M2/4‑1 (MQ=255)  tggattgatcttgtcgaaccggacgacgacgagcggctgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCct                                                                                                                                        <  1:894151‑M2/5‑1 (MQ=255)     attgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttct                                                                                                                                     >  1:463665‑M2/136‑143 (MQ=255)      ttgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgaccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttctt                                                                                                                                    >  1:548019‑M2/135‑143 (MQ=255)       tgagcgtgtcgacccggacggcgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactgtaagacatcgaagcatcggcacgtttctgtgaagacgacgacggcctgcatCTCcttcttc                                                                                                                                   <  2:238671‑M2/10‑1 (MQ=255)          tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttc                                                                                                                                <  2:607505‑M2/13‑1 (MQ=255)          tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcctc                                                                                                                                >  1:471723‑M2/131‑143 (MQ=255)           cttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttct                                                                                                                               >  1:487556‑M2/130‑143 (MQ=255)             tgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCttt                                                                                                                             <  1:160588‑M2/16‑1 (MQ=255)              gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgcagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                            >  1:632532‑M2/127‑143 (MQ=255)                cgagccggacgacgacgagcgactgcgcgtacaatctgaagttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGaa                                                                                                                          <  2:441359‑M2/19‑1 (MQ=255)                   accggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaacgggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGAt                                                                                                                       <  1:235128‑M2/22‑1 (MQ=255)                    ccggacgacgacgagcgactgcgcgtacaatctgaacttggccagcgcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgtagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATg                                                                                                                      <  1:40242‑M2/23‑1 (MQ=255)                       gacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcattgggacgtctatctgaagacgacgacggcctgcaaCCCCTGCTTCTTCTTTGAAGATGCgg                                                                                                                   >  2:717249‑M2/118‑143 (MQ=255)                          gacgacgcgcgtctgcccgtacaatctgaacttggccagagcctggcatcccgcccggtactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAg                                                                                                                <  2:247164‑M2/29‑1 (MQ=255)                           acaacgatcgactgccaagacaatctgaacttggccagagcctggcaacccgcccgtaactggaagacatcgatgcatcgccacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGa                                                                                                               <  2:133185‑M2/30‑1 (MQ=255)                             gacgagcgactgcgcgtacaatctgagcttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATc                                                                                                             <  1:699847‑M2/32‑1 (MQ=255)                               cgagcgcctgcgcgtacaatctgaactttgccagagcctggcaacccgctcggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCAc                                                                                                           <  2:765455‑M2/34‑1 (MQ=255)                                     agtgggggtacgatctgagctgggcccgagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGt                                                                                                     <  2:300129‑M2/40‑1 (MQ=255)                                     actgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagaccacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGt                                                                                                     <  1:497735‑M2/40‑1 (MQ=255)                                         cgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTACCt                                                                                                 >  1:376182‑M2/100‑143 (MQ=255)                                              acaatctgcacctgcctatagcctgggaacccgccctgaactggaagacatagaagcttcggctcgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACt                                                                                            <  2:333897‑M2/49‑1 (MQ=255)                                                 ttctgagcttggcgagggcctggcatcccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgtg                                                                                         <  2:778516‑M2/52‑1 (MQ=255)                                                 atctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgtg                                                                                         >  1:831616‑M2/92‑143 (MQ=255)                                                 atctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGCATCTCCACtgtg                                                                                         >  2:690515‑M2/92‑143 (MQ=255)                                                     gaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAt                                                                                     >  1:242470‑M2/88‑143 (MQ=255)                                                          tggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatATCCTTCTTCTTCTTTAAAGATGCGGAAGATCACGACGGAAACTCCACAGTGAAATTTAcc                                                                                >  1:766388‑M2/83‑143 (MQ=255)                                                            gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCGCTGTGGCATTTACCAt                                                                              >  2:659199‑M2/81‑143 (MQ=255)                                                            gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCCGTAACGCCACCGTGGCATTTACCAt                                                                              >  2:661672‑M2/81‑143 (MQ=255)                                                             ccagggcctggcaccccgcccggaacgggaggacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATc                                                                             <  2:587884‑M2/64‑1 (MQ=255)                                                             ccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCatg                                                                             >  1:843296‑M2/80‑142 (MQ=255)                                                              cagagcctgtctactctccctgaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATcc                                                                            <  1:61353‑M2/65‑1 (MQ=255)                                                              cagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGATGATCACGCCGGTATCTCCTCTGTGTCATTTCCCAtca                                                                            >  1:101703‑M2/79‑142 (MQ=255)                                                                   cctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAt                                                                       <  1:191772‑M2/70‑1 (MQ=255)                                                                   cctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCGCTGTGGCCCCTCCCatccgcgtt                                                                       >  1:252405‑M2/74‑139 (MQ=255)                                                                            ccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGCCTg                                                              >  2:942220‑M2/65‑143 (MQ=255)                                                                                      ctggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCTTGATGGTCGTCTGTTTACTCTgc                                                    <  2:662608‑M2/89‑1 (MQ=255)                                                                                      ctggaagacatagaagtctcggcacgtttctttgaagacgacgactgcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACTATCCGTGATGGTCGTCTGTTTACTCTgc                                                    <  2:95372‑M2/89‑1 (MQ=255)                                                                                                  gaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAAc                                        <  2:140046‑M2/101‑1 (MQ=255)                                                                                                  gaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAAc                                        <  1:302003‑M2/101‑1 (MQ=255)                                                                                                      catcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGCGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGcc                                    >  1:274788‑M2/39‑143 (MQ=255)                                                                                                               gtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTCCTCTGCGTGAGCGTGAACTACCCgcttttag                           >  1:881526‑M2/30‑141 (MQ=255)                                                                                                                tttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGt                          <  1:663484‑M2/115‑1 (MQ=255)                                                                                                                 ttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGAAGATAACTGCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTCTACTCTGCGTGAGCGTGCGCTGCacgcttttagca                         >  2:157971‑M2/28‑139 (MQ=255)                                                                                                                      ttaagacaatgtcggcctgcatCTCCGTATTCTTCTGTGAAGAGGCGGAAGAGCACGCCGGTAACGCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTTCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTAt                    <  1:100747‑M2/121‑1 (MQ=255)                                                                                                                        aagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTACACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCg                  <  2:487556‑M2/123‑1 (MQ=255)                                                                                                                          gacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa                >  1:803708‑M2/19‑143 (MQ=255)                                                                                                                           gcggtggcggcatgcatCTCTTTCTCCTTCTTGGGAGATGCGGAAGATCACGCCGGTAACTCCACTGTCGCAGTTACCATCCGTCACGGTCGTCCGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAt               <  2:361591‑M2/126‑1 (MQ=255)                                                                                                                               cgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTCCGTCTGTATCGTATGCGt           >  1:350812‑M2/14‑143 (MQ=255)                                                                                                                                   ggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGccc       <  2:480574‑M2/134‑1 (MQ=255)                                                                                                                                     cctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGt     >  1:511310‑M2/8‑143 (MQ=255)                                                                                                                                        gcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTCACCTGCCCGCTTCTCGTCTGTATCGTCTGCTTgcccgacgc  >  1:289749‑M2/5‑139 (MQ=255)                                                                                                                                            |                                                                                                                                           TTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGC  >  NC_000913/4001511‑4001788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑