Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,992,524 (CGGGC)2→1 coding (1372‑1376/2547 nt) cyaA → adenylate cyclase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3992522 =2 (0.030)48 (0.830) 45/270 0.2 96.1% coding (1370/2547 nt) cyaA adenylate cyclase
?NC_000913 = 3992516 2 (0.030)coding (1364/2547 nt) cyaA adenylate cyclase

TCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACC  >  NC_000913/3992375‑3992658
                                                                                                                                             |                                                                                                                                              
tctcCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCGTTATTTATGTGCCg                                                                                                                                               <  1:592628‑M2/143‑2 (MQ=255)
    cGCCGGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgg                                                                                                                                           >  2:315026‑M2/1‑138 (MQ=255)
     gCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCAACCGATCTCTCGGAACCGAATATGACCTTTATTCACGTGCCgccggg                                                                                                                                          <  2:492862‑M2/143‑7 (MQ=255)
         ggccgcGGGAGATCGGCGTGCTGACGCGTGAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaa                                                                                                                                      >  2:115078‑M2/3‑133 (MQ=255)
          tCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGGGCCGCTGGTAAGCCCGCAGATGTCACCCGATCTCTCGGAACCGAAGCTGACCCTTATGTATGGGGCgccgggctaac                                                                                                                                     <  2:807630‑M2/143‑12 (MQ=255)
           ccGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACGAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGACCCGAGTCTGACCATAATTTAGGTGTCgccgcgctgacc                                                                                                                                    <  2:30167‑M2/143‑13 (MQ=255)
             gcagcaTATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCCGATTTCGCCCGATCTCTCGGAACCGAATCTGACCTTGATTTATGTGCCgccgggctaaccgt                                                                                                                                  >  2:186432‑M2/1‑129 (MQ=255)
             gCAGTATATCGGCTTGCTGAGGCGTAAGCTGTATGCCGCGTTTGAAGAATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgt                                                                                                                                  >  2:965408‑M2/1‑129 (MQ=255)
             gCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgt                                                                                                                                  <  1:870648‑M2/143‑15 (MQ=255)
               aGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttc                                                                                                                                >  2:576680‑M2/1‑127 (MQ=255)
                     tCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCAAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttg                                                                                                                          >  2:455385‑M2/1‑121 (MQ=255)
                             cTGACGCGTAAGCGGGATGCCGCGTTCGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgt                                                                                                                  >  1:219245‑M2/1‑113 (MQ=255)
                              tGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgta                                                                                                                 <  1:181570‑M2/143‑32 (MQ=255)
                              cgaCGCGTAAGCTGGATGCCGCGTTTGAAGCACTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgta                                                                                                                 >  1:1022492‑M2/2‑112 (MQ=255)
                                 cgcgTCGGCTGTAGGCCGCGTTTGACGCATTACCAGGTAAAGTGACGCTGGTAACCCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataa                                                                                                              >  2:645271‑M2/1‑109 (MQ=255)
                                   cgTAAGCGGTATGCCGCGTTTGAAGCATCACCAGGTAAAGTGACGCTGGTAAACCCGCAGAGTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataacc                                                                                                            >  1:366982‑M2/1‑107 (MQ=255)
                                     tAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTCTGTGCCgccgggctaaccgttcaggttggtatctgtataaccgc                                                                                                          <  2:651272‑M2/143‑39 (MQ=255)
                                       aGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAACCCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgc                                                                                                        >  1:522290‑M2/1‑103 (MQ=255)
                                         cTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGGAAACCCGCAGATTTCACCCGATCTCGAGGAACCGAATCGGACCTTTATTTAGGTGCCgccgggctaaccgttagggttggtatcggtgtaaccgcgcgg                                                                                                      <  1:28773‑M2/143‑43 (MQ=255)
                                              tGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaat                                                                                                 <  1:592781‑M2/143‑48 (MQ=255)
                                                  gcgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaagctga                                                                                             <  1:30800‑M2/143‑52 (MQ=255)
                                                     gttgAAGCATCACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagt                                                                                          >  1:642046‑M2/2‑89 (MQ=255)
                                                        caacctttACCATCTAAATCGACTCTTCTAATCCCCCATATTTCACCCGTTCTCTCGTAACCGAATCTGACCTTTATTTATGTGCCTCCG‑‑‑C‑‑TCTAACCTTTCATTTTGGTATCTTTATAACCTCTCGCCAAATATTGAGTCGa                                                                                  <  2:800062/137‑1 (MQ=255)
                                                         aaGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgat                                                                                      >  1:668421‑M2/1‑85 (MQ=255)
                                                              ttACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatca                                                                                 <  2:637125‑M2/143‑64 (MQ=255)
                                                              ttACCAGGTAAAGGGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatca                                                                                 >  1:483751‑M2/1‑80 (MQ=255)
                                                                   aGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccat                                                                            >  1:1079583‑M2/1‑75 (MQ=255)
                                                                    ggTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgcccaagattgagtcgatcatccgccatc                                                                           <  2:613775‑M2/143‑70 (MQ=255)
                                                                        aggtgACTCGGATTAACCGGCAGATGGCCCCCGCTCTCTCGGGACCGAATCTGACTTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctctataaccgcgcgccaaatattgagtggatcatcagccatcagcc                                                                       >  2:968952‑M2/3‑70 (MQ=255)
                                                                           tGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgct                                                                    >  2:923321‑M2/1‑67 (MQ=255)
                                                                               gCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgagccaactatcgagtcgatcatcaggcatcagccgctggaa                                                                <  2:975900‑M2/143‑81 (MQ=255)
                                                                                  ggTAAACCCGCAGATTTCACCCGTTCGCTCTTAACCGAATCTGACCTGTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatat                                                             >  1:682853‑M2/1‑60 (MQ=255)
                                                                                    tAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgctcgccaaatattgagtcgatcatcagccatcagccactggaatataa                                                           <  1:637062‑M2/143‑86 (MQ=255)
                                                                                      aaCCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaacattgagtcgatcatcagccatcacccgcgggaatataacc                                                         <  2:746612‑M2/143‑88 (MQ=255)
                                                                                         ccGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgtt                                                      <  1:32548‑M2/143‑91 (MQ=255)
                                                                                          cGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgtta                                                     <  1:478194‑M2/143‑92 (MQ=255)
                                                                                           gCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttgc                                                    <  1:403588‑M2/143‑93 (MQ=255)
                                                                                           gCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttac                                                    >  1:269839‑M2/1‑51 (MQ=255)
                                                                                                tttCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTACGTGC‑‑CC‑‑‑CCGGGCTCACCGTTCAGGCTGGTATCTGTATAACCGCGCGCCAACAATTGCGCCGATCATCTGCCACCCGCCGCTGGGCTAAAACCCttctccgac                                          >  2:194023/1‑136 (MQ=255)
                                                                                                     cccGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagccgatcatcggccatcagccgccggaatatcaccgttacctggctaacc                                          <  1:121822‑M2/143‑103 (MQ=255)
                                                                                                      ccGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagacatcagccgctggaatataaccgttacctgaataaact                                         <  1:821688‑M2/143‑104 (MQ=255)
                                                                                                       cGAGCGCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggcgaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactg                                        >  2:870648‑M2/1‑39 (MQ=255)
                                                                                                          gctctcgGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtacaaccgcgcgccaaatattgagtcgatcatcagccatcagccgctgggatataaccgttacctgaataaactggtg                                     >  1:596871‑M2/2‑36 (MQ=255)
                                                                                                             ctcGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcg                                  <  2:578232‑M2/143‑111 (MQ=255)
                                                                                                             ctcGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataacagcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgactaacctggtggcg                                  <  2:130525‑M2/143‑111 (MQ=255)
                                                                                                              tcGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgt                                 <  1:1039795‑M2/143‑112 (MQ=255)
                                                                                                                   cacggATCTGGCCTTGATTTCGGCGCCgcggggctaaccttgcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggca                            >  2:28773‑M2/3‑27 (MQ=255)
                                                                                                                                 ttatttatGTGCCgccgggctaaccgttaaggttgggttctgtataaccgcgcgccaaatattgagtcgatcatcggccatcagccgctggaatataaccgttacctgaataaactgggggcgtgggcatggtttaactgcct              <  2:898202‑M2/143‑131 (MQ=255)
                                                                                                                                  tatttatGTGCCgccgggcggaccgttgaggttgggatctgtataaccgcgcgccaaatattgagtcgatcatcagccagcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctg             >  2:671275‑M2/1‑12 (MQ=255)
                                                                                                                                      gcggggccgccgggctaaccgtgcaggttggtatctgtagaaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctgctga         >  1:271328‑M2/6‑8 (MQ=255)
                                                                                                                                           cccgccgggctaaccgttcaggctggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctgctgacctcg    >  2:554825‑M2/2‑3 (MQ=255)
                                                                                                                                            ccgccgggctaaccgttcaggtgggtatctgtacaaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctgctgacctcgc   >  1:91520‑M2/1‑2 (MQ=255)
                                                                                                                                             cgccgggctagccgttcaggttggtatctgtataaccgcgcgccgaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctgctgacctcgcg  >  2:753277‑M2/1‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
TCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACC  >  NC_000913/3992375‑3992658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.