Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258675 258683 9–776 48 [0] [0] 49 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)47 (1.150) 41/268 0.1 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

TTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913/258556‑258826
                                                                                                                                |                                                                                                                                              
caaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgctaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcgcgtatattcgcgaaggtaAGTGCAAAGCTAATc                                                                                                                                  >  2:749994‑M2/129‑143 (MQ=255)
          caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactgggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        <  1:265121‑M2/25‑1 (MQ=255)
          caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                                <  2:427013‑M2/1‑1 (MQ=255)
          caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                                >  1:427013‑M2/119‑119 (MQ=255)
          caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        <  1:580664‑M2/25‑1 (MQ=255)
          caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        <  1:227400‑M2/25‑1 (MQ=255)
           aatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatcttcgtgaaggtcAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                       >  1:51265‑M2/118‑143 (MQ=255)
             tttggtaaaacagttgcatcacaacaggagatagcaatgacgttacagagtggacacccgaagagcagagtgataaaaaaatttaccgcactaggcccgtagatgagtgaagggaAGTGCGGAGATACTCGCTTCTTTttctc                                                                                                                     >  2:751142‑M2/116‑141 (MQ=255)
                  taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggatcgtataatcaacaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                >  1:289852‑M2/111‑143 (MQ=255)
                   aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccggatattcgtgaaggtaAGTGCAAAGATAATCGATACTATTTCGATTGTCt                                                                                                               >  1:506126‑M2/110‑143 (MQ=255)
                    aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAACATAATCGCTTCTTTTTCGATTGTCTg                                                                                                              >  1:115538‑M2/109‑143 (MQ=255)
                     aacagttgcatcacaacaggagatagcaatgaccttaccgagtggcaacccgaacagcagattgatcaaaaaacttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                             <  2:436920‑M2/36‑1 (MQ=255)
                      acagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTTTGGc                                                                                                            >  2:140374‑M2/107‑143 (MQ=255)
                       ctgttgaataacaaaaggagttagcaatgacgtgaccaggtggacacccgaagagtatattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                           <  2:33517‑M2/38‑1 (MQ=255)
                               tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGAAAAGATAATCGATTCTTTTTAGATTGGCTGGGTGTATGCGt                                                                                                   >  1:243061‑M2/98‑143 (MQ=255)
                                     caggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGAGTCAACGt                                                                                             >  2:237796‑M2/92‑143 (MQ=255)
                                          gatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                                                                        >  1:34552‑M2/87‑143 (MQ=255)
                                                 atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTAGCTGTATGCGTCAACGTGAAACCGGCAcc                                                                                 >  2:142492‑M2/80‑143 (MQ=255)
                                                     cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                             <  2:480965‑M2/68‑1 (MQ=255)
                                                           cgagtgggcacccgaagagcagattgagcaaaaaatttaccgcacttggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                       <  2:480449‑M2/74‑1 (MQ=255)
                                                           cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaGGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTGTGCGGCAACGTGAAGCCGGCCCCGGGAGTGCGt                                                                       >  1:717552‑M2/70‑143 (MQ=255)
                                                                    acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTgg                                                              >  1:689293‑M2/61‑143 (MQ=255)
                                                                    acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                              >  1:593695‑M2/61‑143 (MQ=255)
                                                                    acccgaagagcagattaatcaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                              <  1:140849‑M2/83‑1 (MQ=255)
                                                                    acccgaagaccagattgatcaaaaaatttaccgaactaggcccgcatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                              <  1:685423‑M2/83‑1 (MQ=255)
                                                                      ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGACACCGGCACCGGAAGTGCGTGAATTCTgggg                                                            >  1:501641‑M2/59‑143 (MQ=255)
                                                                         aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTATCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGCATTCTGGGGCtg                                                         >  2:631599‑M2/56‑143 (MQ=255)
                                                                          agagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAGAGATAGTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCCACGTGAAACCGGCACCGGACGTGCGTGAATTCTGGGGCtgg                                                        >  1:232105‑M2/55‑143 (MQ=255)
                                                                             gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                     <  1:423131‑M2/92‑1 (MQ=255)
                                                                              cagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                    <  1:543525‑M2/93‑1 (MQ=255)
                                                                                 attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg                                                 >  1:34467‑M2/48‑143 (MQ=255)
                                                                                   tgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg                                               <  1:27604‑M2/98‑1 (MQ=255)
                                                                                   tgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGACGGAg                                               >  2:497038‑M2/46‑143 (MQ=255)
                                                                                     cgcaaaaaatttgccggcctaggcgcgtatattggtgaaggtaGGTGCAAGGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTGAACGTGGAACCGGCACCGGGAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                             <  2:567581‑M2/100‑1 (MQ=255)
                                                                                       caaaaaatgtaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                           <  2:590999‑M2/102‑1 (MQ=255)
                                                                                        aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCCGAAGTGCGTGAATCCTGGGGCTGGGGGATGGAGCaggg                                          >  2:252430‑M2/41‑142 (MQ=255)
                                                                                         aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTGTACGTGAAACCGACACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                         >  1:177323‑M2/40‑143 (MQ=255)
                                                                                         aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                         <  1:251104‑M2/104‑1 (MQ=255)
                                                                                              tttaccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                    <  1:189978‑M2/109‑1 (MQ=255)
                                                                                                            cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGGAGGGCTGGTATGTTTGGTCTGGTGGATGGAGCTGGGAGCGCCGGAATCCAGTTTTa                      >  1:146018‑M2/21‑143 (MQ=255)
                                                                                                             ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     >  1:463377‑M2/20‑143 (MQ=255)
                                                                                                             acgtatattcgtgtaggtaAGTGCAAAGTTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     <  2:608983‑M2/124‑1 (MQ=255)
                                                                                                                  tattcgtgaaggtaGGGGAAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                <  1:604682‑M2/129‑1 (MQ=255)
                                                                                                                  tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGAAAGCGCAGGCATCCCGGCTTACCTacc                >  2:744802‑M2/15‑142 (MQ=255)
                                                                                                                  gattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTAGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                <  1:749994‑M2/129‑1 (MQ=255)
                                                                                                                    ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt              >  1:624397‑M2/13‑143 (MQ=255)
                                                                                                                      cgtgaaggtaAGTGCAAAGATAATCGAGTCTTTTTCGATGGTCTGGCTGTATGCGTAAACGCGAAACCGGCACCGGAAGGGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa            <  1:362044‑M2/133‑1 (MQ=255)
                                                                                                                                aGTGCAAAGGTAATCGATTCTTCTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGACGTGCGCGAATTCTGCGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt  <  2:506126/143‑1 (MQ=255)
                                                                                                                                aGTGCAAAGAGAATCGATTCTTTTGCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCGCCGGAAGTGCGGGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt  <  2:51265/143‑1 (MQ=255)
                                                                                                                                |                                                                                                                                              
TTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913/258556‑258826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.