Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 48 [0] [0] 49 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)45 (1.000) 40/266 0.1 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913/258542‑258826
                                                                                                                                              |                                                                                                                                               
tgttaaaaattttctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                                 <  1:344506‑M2/1‑1 (MQ=255)
            gctaaattttgccaatttggtccaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATaa                                                                                                                                     <  2:213715‑M2/13‑1 (MQ=255)
             ctaaattttgccaattgggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                                                                    <  1:755871‑M2/14‑1 (MQ=255)
              taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgacgagcagattgatcaaaaaatttaccgcacgacgcccgtatagtcgtgaagctaAGTGCAAAGATAATc                                                                                                                                   >  2:203138‑M2/129‑143 (MQ=255)
               aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagagtgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                                                                  >  2:82980‑M2/128‑143 (MQ=255)
                aattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                                                                 >  1:353644‑M2/127‑143 (MQ=255)
                     cgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccaagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgcgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                                                           <  1:67460‑M2/22‑1 (MQ=255)
                          atttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                                                                      <  1:716588‑M2/27‑1 (MQ=255)
                                     cagttgcatcacaacaggagatagcaatgacgttaccgagtggacagcgggggggcacacatatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                           <  1:403891‑M2/38‑1 (MQ=255)
                                         tgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                                                                       <  1:839759‑M2/42‑1 (MQ=255)
                                           catcacaacgggagatagcaatgacgttaccgagtggacagccgaagagcagattgatcaaaaaatttaccgcactagtcccgtatattcgtgaaggtaATTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                     <  2:194904‑M2/44‑1 (MQ=255)
                                            atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                                                                    >  1:261079‑M2/99‑143 (MQ=255)
                                             ccacagcaggagatagcaatgacgtgaccgagtggacacccgaagagcagattgatcaaaaaatttactgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                   <  2:648192‑M2/46‑1 (MQ=255)
                                                    aggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAGCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                            <  1:557817‑M2/53‑1 (MQ=255)
                                                    agagaatagaaatgacgttacctagtggacacgcgaagagcagattgatcaaaaaattttccgcactaggcccgtatattcgtgaaggtaAGTGCAACGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                            <  2:81533‑M2/53‑1 (MQ=255)
                                                         atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatcttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGCAAcc                                                                                       >  1:242412‑M2/86‑143 (MQ=255)
                                                         atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                       >  1:743623‑M2/86‑143 (MQ=255)
                                                          tagcaatgacgttaccgagtggacacccgaatagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                                                                      <  2:867590‑M2/59‑1 (MQ=255)
                                                           agcaatgacgttaccgaggggacacccgaagagccgagtgatcaaaaaatttcccgcactaggcccgtatattcgtgaaggtaAGTGCAACGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                     <  1:6406‑M2/60‑1 (MQ=255)
                                                           agcaaggacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                     <  1:807730‑M2/60‑1 (MQ=255)
                                                              aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                                                                  <  1:843529‑M2/63‑1 (MQ=255)
                                                                   ggttaccgagtggccacccgaagagcagactgatcaaaaaattgaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                             <  1:188855‑M2/68‑1 (MQ=255)
                                                                    gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAGCTCGGGACCGGCAg                                                                            >  1:183785‑M2/75‑143 (MQ=255)
                                                                       accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGCCTGTCTGCGTCGACGTGAAACCGTCCCCGGAcgtcc                                                                         >  1:728636‑M2/72‑141 (MQ=255)
                                                                        ccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                                                                        <  1:578477‑M2/73‑1 (MQ=255)
                                                                         cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                       <  2:107769‑M2/74‑1 (MQ=255)
                                                                               gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                 <  1:813118‑M2/80‑1 (MQ=255)
                                                                                   cccgaagaccagctttatcaaaacatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTCGATTGTCTGGCTGTATTCGTCAACGTGAAACCGGCACCTGAAGTGCGTGAATTCTgg                                                              <  2:210639‑M2/84‑1 (MQ=255)
                                                                                          agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                      >  1:524799‑M2/53‑143 (MQ=255)
                                                                                               attgatcaaacaatttacctcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg                                                 <  1:209621‑M2/96‑1 (MQ=255)
                                                                                                   atcaaaaaattttccgcactaggcccgtatattcgtaaaagtaAGTGGATGGGTGGTCGATTCTTTTTCGATGGTCGGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                             <  1:239880‑M2/100‑1 (MQ=255)
                                                                                                            tttcccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAGCCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                    <  2:580197‑M2/109‑1 (MQ=255)
                                                                                                                acgaaatagccccggatgttcgtgaaggtaAGTGGAAAGACAATCGATGCTGGTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGGACCGGAAGAGCGTTAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGaa                                <  2:392385‑M2/113‑1 (MQ=255)
                                                                                                                 cgcactaggcccgtatattcgtgaacgtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTCACTCCTGCGGCTGGTGGATGAAGCCGCACCCgcaggccc                               >  2:611497‑M2/30‑140 (MQ=255)
                                                                                                                     ataggcccctatattcgtgaaggtaAGTGCAAAGCTACACGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCg                           <  2:710567‑M2/118‑1 (MQ=255)
                                                                                                                      taggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGGTGGAGCTGGAAGCGCAGGAATCCCGt                          >  1:469416‑M2/25‑143 (MQ=255)
                                                                                                                       aggcccgtatattcgtgaaggtaAGTTCCAAGATAATAGAAGTGTGGCGGGTGGTCCGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                         <  1:732239‑M2/120‑1 (MQ=255)
                                                                                                                           ccgtgtattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     <  1:747004‑M2/124‑1 (MQ=255)
                                                                                                                           ccgtatgtttgtgaaggtaAGTGCAAAGATAATCGATGCTTTTTCGATGGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     <  2:560454‑M2/124‑1 (MQ=255)
                                                                                                                           ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAAACGAACACGGTGTGGTTTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     >  1:639613‑M2/20‑143 (MQ=255)
                                                                                                                             gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGACACCGGCACCGGACGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCt                   >  1:78896‑M2/18‑143 (MQ=255)
                                                                                                                                tattcgtgtaggtaAGTGCAAGGATAATCGATTCTTTTTCGATGGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                <  2:408233‑M2/129‑1 (MQ=255)
                                                                                                                                tattcgggagggtaAGTGCACAGACAATCGATTCTTTTTCGATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGTATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                <  2:75601‑M2/129‑1 (MQ=255)
                                                                                                                                 attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGACTGGTGGCTTGGGCTGGAAGCGAAGGAATCTCGTTTTCCTTACAg               >  1:542228‑M2/14‑143 (MQ=255)
                                                                                                                                   tcgtgaagggaAGCGCAAAGAGAATCGATTCTTTTTCGAGTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATGCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGtt             <  1:76657‑M2/132‑1 (MQ=255)
                                                                                                                                     gcgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc           <  1:64031‑M2/134‑1 (MQ=255)
                                                                                                                                       gcacgtaAGTGCAAAGCTAATCGATTCTTTTTCGATTGTCGGGCTGTATGCGTCAACGTGGAACCGGCACCGGACGTGCGTGAATTCTGGGGCTGGTGGACGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCa         <  2:165269‑M2/136‑1 (MQ=255)
                                                                                                                                          gggaAGTGCAAAGATAATCGATGCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGtt      <  1:149392‑M2/139‑1 (MQ=255)
                                                                                                                                              aGTGCAAAGATAATCGATTCTGTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt  <  1:740281/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913/258542‑258826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: