Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 700,597 IS1 (–) +9 bp coding (990‑998/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)40 (0.810) 36/284 0.4 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 700605 0 (0.000)coding (990/1221 nt) nagC N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
* ? NC_000913 = 258675NA (NA)61 (1.230) 49/284 0.0 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 700597 = 0 (0.000)coding (998/1221 nt) nagC N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGA  >  NC_000913/258536‑258678
                                                                                                                                           |   
gcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTgg  >  1:223963/1‑142 (MQ=11)
                                                                                                                                           |   
GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGA  >  NC_000913/258536‑258678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.