New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1159325 =	56 (1.190)	5 (0.110)	5/262	5.7	8.9%	intergenic (+23/+37)	ptsG/fhuE	fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
	?	NC_000913	1159347 =	50 (1.140)					intergenic (+45/+15)	ptsG/fhuE	fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGGGAACGTCAATAACCTGTTCGACAAAACCTACGATACCAACGTGGAAGGTTCTATCGTCTACGGCACACCGCGTAATTTCAGCATTACCGGCACGTATCAATTCTGAATAACACCTGTAAAAAAGGCAGCCATCTGGCTGCCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1159470‑1159325 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggctgccttTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTC  >  NC_000913/1159347‑1159488                                                                                                                                                                                                                                                                                                  GGGGAACGTCACTAACCTGTTCGACAAAACCTACGATACCAACGTGGAAGGTTCTATCGTCTACGGCACACCGCGTAATTTCAGCATTACCGGCACGTATCAATTCTGAATAACACCTGTAAAAAAGGCAGCCATCTGGCTGC                                                                                                                                                   <  1:613116/143‑1  GGGAACGTCAATAACCTGTTCGACAAAACCTACGATACAAACGTGGAAGGTTCTGTCGTCTACGGGACACCGCGTAATTTCAGCATTACCGGCACGTATCAATTCTGAATAACACCTGTAAAAAAGGCAGCCATCTGGCTGCC                                                                                                                                                  >  1:725553/1‑143                                                                                           CGGCACGTATCAATTCTGAATAACACCTGTAAAAAAGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGGTGGT                                                         >  1:14245/1‑143                                                                                               ACGTATCAATTCTGAATAACACCTGTAAAAAAGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATCCTGAAATTACGCGGTCTGCCGTCGACGATAGAACCTTCCACGTTGGTCTCG                                                     >  2:375586/1‑143                                                                                                                           AAAAGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTAGTATCGTAGGTTTTGTCGAAACGGTTATTGACGT                         >  2:123823/1‑143                                                                                                                            AAAGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTT                        >  1:582772/1‑143                                                                                                                               GGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTGGGTATCGTCGGTTGTGTCGAACAGGTTGTTAACGTTCCC                     >  1:439582/1‑143                                                                                                                                          GGCTGCCCTTTTTAGCGGTGTTATTCAGAATGGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGA          <  2:120622/143‑1                                                                                                                                               CCTTTTTTACAGGTTTTATTCAGATTTGTTTCGTGTCGGTAATGCTGATATTACGGGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTATGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTT     <  2:231070/143‑1                                                                                                                                                  TTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGGAGTTTTTC  >  2:341560/1‑143                                                                                                                                                                                                                                                                                                  GGGGAACGTCAATAACCTGTTCGACAAAACCTACGATACCAACGTGGAAGGTTCTATCGTCTACGGCACACCGCGTAATTTCAGCATTACCGGCACGTATCAATTCTGAATAACACCTGTAAAAAAGGCAGCCATCTGGCTGCCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1159470‑1159325 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggctgccttTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTC  >  NC_000913/1159347‑1159488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.