New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3349143	36 (0.830)	4 (0.090)	4/276	5.9	10.6%	coding (665/729 nt)	mtgA	biosynthetic peptidoglycan transglycosylase
	?	NC_000913	= 3349177	32 (0.760)					coding (631/729 nt)	mtgA	biosynthetic peptidoglycan transglycosylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAG  >  NC_000913/3349012‑3349281                                                                                                                                    |                                                                                                                                           cTATTCCCCTGCAGAAAGATCTGCACCCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACgg                                                                                                                                 <  2:508430/143‑1 (MQ=255)  tATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGGTTAATCCAGCGGGTGTTGTTGCATAAACGGCGCATCGCCTAACTGATACATCTACCGTAAAATCCACGCCTGACggg                                                                                                                                >  2:163577/1‑142 (MQ=255)               aaaGATCTGCAACCTCGTGAGAGCAAAACGCGGGCGCGCACTGCGCGCGAAGCGTAGTCCAGCTGGTGTTGTTGCATAAACGGCCCACCGCCTAACTGATACAGCGGCCGGAAACTCCACGCCGGAGGGCCACGCACGTAGcc                                                                                                                   >  1:622366/1‑143 (MQ=255)                   aTCTGCAACCGCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGTGCGTAGccgtca                                                                                                               <  1:509511/143‑5 (MQ=255)                   aTCGGCGACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGAt                                                                                                               <  1:45208/143‑1 (MQ=255)                    tctcCAACGTCGTCGGATCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGGTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATg                                                                                                              <  1:453600/143‑1 (MQ=255)                        cAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCga                                                                                                          <  2:608120/143‑1 (MQ=255)                         aaCTTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCgag                                                                                                         <  2:268467/143‑1 (MQ=255)                         aaCCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCgag                                                                                                         <  2:648363/143‑1 (MQ=255)                                ggagaGCAAAACGCTGGCCCTCGCTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTGGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACtt                                                                                                  <  1:410520/142‑1 (MQ=255)                                 gagaGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACttt                                                                                                 >  2:419634/1‑143 (MQ=255)                                         aaCGCTGGCCCTCGCTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGaa                                                                                         <  2:418011/143‑1 (MQ=255)                                         aaCGCTGGCCCTCACTTCGCGCGCAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGCCGGCTACACACGTACCGCGATGACGAGGAGACGTTCAAACGat                                                                                         >  1:79276/1‑142 (MQ=255)                                                   cTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATACACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTCGCCCGATGGCGAGGCGACGTTGAAACGAAGAGGACTAgc                                                                               >  1:75638/1‑142 (MQ=255)                                                        ttCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGCGACTTTGAAACGAAGTGGATTAGGTAATa                                                                          >  2:125792/1‑143 (MQ=255)                                                                   cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGCCTTTGAAACGACGTGGATTAGGGAATAGAGCTGCCAGt                                                               >  1:426972/1‑143 (MQ=255)                                                                   cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGt                                                               >  2:271429/1‑143 (MQ=255)                                                                   cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGCGGATTAGGTAATACAGCTGCCAGt                                                               >  2:96225/1‑143 (MQ=255)                                                                       gtCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATg                                                           <  2:723584/142‑1 (MQ=255)                                                                           aGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGCTGCTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAgg                                                       >  2:45606/1‑142 (MQ=255)                                                                                           tAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTCGCCCGATGGCGACGCGACTTTGAAACGAAGTGGATTAGGTAATACATCTGCCAGTAATGCAGCTTCCGACCGGGaaaat                                       >  2:529917/1‑141 (MQ=255)                                                                                           tAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAGTACAGCTGCCAGTAATGCAGCTTCCGCCCGGGTAAGt                                       >  2:600919/1‑143 (MQ=255)                                                                                            aaaCGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGtt                                      >  2:741933/1‑143 (MQ=255)                                                                                                ggatCACCGCCTAACTCATACATCTGCCGTAAATTCCCCGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCt                                  <  1:33767/140‑1 (MQ=255)                                                                                                     accgtcggaCTCATACAGCCGCCGTAAAATCCACGCCGGATGGCTAGGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTATTACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCgg                             <  2:728148/135‑1 (MQ=255)                                                                                                     aCCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGCCTTTGAAACGAAGCGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCCcgcgc                             >  1:133756/1‑142 (MQ=255)                                                                                                     aCCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCgg                             >  2:230421/1‑143 (MQ=255)                                                                                                     aCCGCCTAACTGATACAGCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCgg                             <  1:709888/143‑1 (MQ=255)                                                                                                           taactgcgcccgccgCCGTGAAGTCCGGGCCTGACGGGTAGGCACGTAGCGCGAGGGCGAGGAGACTTTGAAACGAAGGGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTtgt                       <  1:43570/129‑1 (MQ=255)                                                                                                             aCTGATACATCTGCCGTAAAATACACGCATGACGGGTACGCACGTAGCACGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCGAGTAATGCAGCTTAGGAGCGGGTAAGTTTGCTAGCGGGAtggtgg                     >  2:257511/1‑143 (MQ=255)                                                                                                                     aTCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCGGTAATGCAGCTACCGACAGGGGAAGTTAGCTCGCGGGGTTGTGGAaacaaca             >  1:514860/1‑142 (MQ=255)                                                                                                                          ccGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTtgtg        <  1:52328/143‑1 (MQ=255)                                                                                                                             tAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCATTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCg     >  1:373970/1‑143 (MQ=255)                                                                                                                                aacccAGGCGTGGCGGGTACGCAGGTAGGCCGAGGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAg  <  2:305414/140‑1 (MQ=255)                                                                                                                                aaTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTTTGGAACTACCGTTGTGCCGCAg  >  1:494191/1‑143 (MQ=255)                                                                                                                                aaTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAg  >  2:252977/1‑143 (MQ=255)                                                                                                                                    |                                                                                                                                           CTATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAG  >  NC_000913/3349012‑3349281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.